Mechanistic insights into nitrogen-induced changes in pasting characteristics of rice during storage based on proteomics analysis
Nitrogen application delays rice quality deterioration due to changes in its pasting characteristics; however, the underlying mechanisms remain unclear. Using a label-free quantitative proteomics approach, we identified differentially expressed proteins (DEPs) during storage in paddy rice treated wi...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Food Chemistry: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157523004613 |
| _version_ | 1797383657979117568 |
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| author | Hanling Liang Baiyu Gu Wentao Sun Bo Li Hang Qu Dongbing Tao Qi Zhang Tianyu Wang Yichao Ma Yajie Wang Zhaoxia Wu Qinghai Zhang |
| author_facet | Hanling Liang Baiyu Gu Wentao Sun Bo Li Hang Qu Dongbing Tao Qi Zhang Tianyu Wang Yichao Ma Yajie Wang Zhaoxia Wu Qinghai Zhang |
| author_sort | Hanling Liang |
| collection | DOAJ |
| description | Nitrogen application delays rice quality deterioration due to changes in its pasting characteristics; however, the underlying mechanisms remain unclear. Using a label-free quantitative proteomics approach, we identified differentially expressed proteins (DEPs) during storage in paddy rice treated with different nitrogen levels. On combining the changes in physiological indicators, high-nitrogen treatment was found to downregulate β-1,3-glucanase, reduce the decomposition of cell wall components, downregulate three proteins involved in starch metabolism, decrease the range of the amylose content and increase the range of the amylopectin, upregulate three proteins related to the lysosomal pathway, and enhance glutelin degradation. In addition, it upregulated three proteins related to flavonoid synthesis, which enhanced the stress response ability of rice, thereby contributing to the stability of biological macromolecules. The discovery of these key DEPs provides potential targets for further control over the deterioration of crop seed storage quality. |
| first_indexed | 2024-03-08T21:24:14Z |
| format | Article |
| id | doaj.art-66379b36e3044165ab49ec46134f63ae |
| institution | Directory Open Access Journal |
| issn | 2590-1575 |
| language | English |
| last_indexed | 2024-03-08T21:24:14Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj.art-66379b36e3044165ab49ec46134f63ae2023-12-21T07:36:59ZengElsevierFood Chemistry: X2590-15752023-12-0120101018Mechanistic insights into nitrogen-induced changes in pasting characteristics of rice during storage based on proteomics analysisHanling Liang0Baiyu Gu1Wentao Sun2Bo Li3Hang Qu4Dongbing Tao5Qi Zhang6Tianyu Wang7Yichao Ma8Yajie Wang9Zhaoxia Wu10Qinghai Zhang11School of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, People’s Republic of ChinaSchool of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, People’s Republic of ChinaInstitute of Plant Nutrition and Environmental Resources, Liaoning Academy of Agricultural Sciences, Shenyang 110161, People’s Republic of ChinaInstitute of Plant Nutrition and Environmental Resources, Liaoning Academy of Agricultural Sciences, Shenyang 110161, People’s Republic of ChinaInstitute of Plant Nutrition and Environmental Resources, Liaoning Academy of Agricultural Sciences, Shenyang 110161, People’s Republic of ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110866, People’s Republic of ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110866, People’s Republic of ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110866, People’s Republic of ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110866, People’s Republic of ChinaSchool of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, People’s Republic of ChinaCollege of Food Science, Shenyang Agricultural University, Shenyang 110866, People’s Republic of China; Corresponding authors.School of Public Health, The Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 550025, People’s Republic of China; Corresponding authors.Nitrogen application delays rice quality deterioration due to changes in its pasting characteristics; however, the underlying mechanisms remain unclear. Using a label-free quantitative proteomics approach, we identified differentially expressed proteins (DEPs) during storage in paddy rice treated with different nitrogen levels. On combining the changes in physiological indicators, high-nitrogen treatment was found to downregulate β-1,3-glucanase, reduce the decomposition of cell wall components, downregulate three proteins involved in starch metabolism, decrease the range of the amylose content and increase the range of the amylopectin, upregulate three proteins related to the lysosomal pathway, and enhance glutelin degradation. In addition, it upregulated three proteins related to flavonoid synthesis, which enhanced the stress response ability of rice, thereby contributing to the stability of biological macromolecules. The discovery of these key DEPs provides potential targets for further control over the deterioration of crop seed storage quality.http://www.sciencedirect.com/science/article/pii/S2590157523004613Proteomics, Rice, Storage, Pasting, NitrogenChemical compounds studied in this articleAmylopectin (PubChem CID: 439207)Globulin (PubChem CID: 131750181)Malondialdehyde (PubChem CID: 10964) |
| spellingShingle | Hanling Liang Baiyu Gu Wentao Sun Bo Li Hang Qu Dongbing Tao Qi Zhang Tianyu Wang Yichao Ma Yajie Wang Zhaoxia Wu Qinghai Zhang Mechanistic insights into nitrogen-induced changes in pasting characteristics of rice during storage based on proteomics analysis Food Chemistry: X Proteomics, Rice, Storage, Pasting, Nitrogen Chemical compounds studied in this article Amylopectin (PubChem CID: 439207) Globulin (PubChem CID: 131750181) Malondialdehyde (PubChem CID: 10964) |
| title | Mechanistic insights into nitrogen-induced changes in pasting characteristics of rice during storage based on proteomics analysis |
| title_full | Mechanistic insights into nitrogen-induced changes in pasting characteristics of rice during storage based on proteomics analysis |
| title_fullStr | Mechanistic insights into nitrogen-induced changes in pasting characteristics of rice during storage based on proteomics analysis |
| title_full_unstemmed | Mechanistic insights into nitrogen-induced changes in pasting characteristics of rice during storage based on proteomics analysis |
| title_short | Mechanistic insights into nitrogen-induced changes in pasting characteristics of rice during storage based on proteomics analysis |
| title_sort | mechanistic insights into nitrogen induced changes in pasting characteristics of rice during storage based on proteomics analysis |
| topic | Proteomics, Rice, Storage, Pasting, Nitrogen Chemical compounds studied in this article Amylopectin (PubChem CID: 439207) Globulin (PubChem CID: 131750181) Malondialdehyde (PubChem CID: 10964) |
| url | http://www.sciencedirect.com/science/article/pii/S2590157523004613 |
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