Three-Phase Partitioning for the Extraction and Purification of Polysaccharides from the Immunomodulatory Medicinal Mushroom <em>Inonotus obliquus</em>
Polysaccharides from the immunomodulatory medicinal mushroom<i> Inonotus obliquus </i>(IOPS) were extracted and purified using three-phase partitioning (TPP), which is an efficient, fast, safe, and green purification technique. An optimal extraction procedure that gave a good 2.2% isolat...
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| Format: | Article |
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MDPI AG
2019-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/3/403 |
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| author | Zhendong Liu Dongsheng Yu Liang Li Xiaoxiao Liu Henan Zhang Wenbo Sun Chi-Chung Lin Jiafu Chen Zhi Chen Wenhan Wang Wei Jia |
| author_facet | Zhendong Liu Dongsheng Yu Liang Li Xiaoxiao Liu Henan Zhang Wenbo Sun Chi-Chung Lin Jiafu Chen Zhi Chen Wenhan Wang Wei Jia |
| author_sort | Zhendong Liu |
| collection | DOAJ |
| description | Polysaccharides from the immunomodulatory medicinal mushroom<i> Inonotus obliquus </i>(IOPS) were extracted and purified using three-phase partitioning (TPP), which is an efficient, fast, safe, and green purification technique. An optimal extraction procedure that gave a good 2.2% isolated yield was identified, using the following protocol: a solid-liquid ratio of 1 g to 12 mL; mass fraction of (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub> 20% (<i>w/v</i>); 11 mL<i> t</i>-butanol; pH 8.0; temperature 30 °C; and extraction time 30 min. The purified IOPS was shown to be a proteoglycan of 40 kDa molecular weight comprising of d-galactose, d-glucose, d-xylose, and d-mannose in a molar ratio of 2.0:3.5:1.0:1.5. The purified IOPS displayed strong free-radical scavenging abilities, antioxidant activities, and immunological activity in vitro. IOPS’ Trolox antioxidant equivalent capacity and ferric-reducing ability of plasma were 251.2 μmol Trolox/g sample and 1040.5 μmol Fe<sup>2+</sup>/g sample, respectively, with the activity of its immunomodulatory behavior shown to be gradient dependent. |
| first_indexed | 2024-12-13T10:56:15Z |
| format | Article |
| id | doaj.art-6639a4bab4914dc2b577269afdee2bab |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-13T10:56:15Z |
| publishDate | 2019-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-6639a4bab4914dc2b577269afdee2bab2022-12-21T23:49:32ZengMDPI AGMolecules1420-30492019-01-0124340310.3390/molecules24030403molecules24030403Three-Phase Partitioning for the Extraction and Purification of Polysaccharides from the Immunomodulatory Medicinal Mushroom <em>Inonotus obliquus</em>Zhendong Liu0Dongsheng Yu1Liang Li2Xiaoxiao Liu3Henan Zhang4Wenbo Sun5Chi-Chung Lin6Jiafu Chen7Zhi Chen8Wenhan Wang9Wei Jia10Food Science College, Tibet Agriculture & Animal Husbandry University, Linzhi, 860000, ChinaInstitute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, ChinaFood Science College, Tibet Agriculture & Animal Husbandry University, Linzhi, 860000, ChinaInstitute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, ChinaInstitute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, ChinaShandong Provincial Key Laboratory of Animal Disease Control & Breeding, Animal Science and Veterinary Medicine Institute of Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong, ChinaInstitute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, ChinaFood Science College, Tibet Agriculture & Animal Husbandry University, Linzhi, 860000, ChinaShandong Provincial Key Laboratory of Animal Disease Control & Breeding, Animal Science and Veterinary Medicine Institute of Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong, ChinaInstitute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, ChinaInstitute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, ChinaPolysaccharides from the immunomodulatory medicinal mushroom<i> Inonotus obliquus </i>(IOPS) were extracted and purified using three-phase partitioning (TPP), which is an efficient, fast, safe, and green purification technique. An optimal extraction procedure that gave a good 2.2% isolated yield was identified, using the following protocol: a solid-liquid ratio of 1 g to 12 mL; mass fraction of (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub> 20% (<i>w/v</i>); 11 mL<i> t</i>-butanol; pH 8.0; temperature 30 °C; and extraction time 30 min. The purified IOPS was shown to be a proteoglycan of 40 kDa molecular weight comprising of d-galactose, d-glucose, d-xylose, and d-mannose in a molar ratio of 2.0:3.5:1.0:1.5. The purified IOPS displayed strong free-radical scavenging abilities, antioxidant activities, and immunological activity in vitro. IOPS’ Trolox antioxidant equivalent capacity and ferric-reducing ability of plasma were 251.2 μmol Trolox/g sample and 1040.5 μmol Fe<sup>2+</sup>/g sample, respectively, with the activity of its immunomodulatory behavior shown to be gradient dependent.https://www.mdpi.com/1420-3049/24/3/403Inonotus obliquusthree-phase partitioningfree-radical scavenging abilitiesantioxidant activityimmunological activity |
| spellingShingle | Zhendong Liu Dongsheng Yu Liang Li Xiaoxiao Liu Henan Zhang Wenbo Sun Chi-Chung Lin Jiafu Chen Zhi Chen Wenhan Wang Wei Jia Three-Phase Partitioning for the Extraction and Purification of Polysaccharides from the Immunomodulatory Medicinal Mushroom <em>Inonotus obliquus</em> Molecules Inonotus obliquus three-phase partitioning free-radical scavenging abilities antioxidant activity immunological activity |
| title | Three-Phase Partitioning for the Extraction and Purification of Polysaccharides from the Immunomodulatory Medicinal Mushroom <em>Inonotus obliquus</em> |
| title_full | Three-Phase Partitioning for the Extraction and Purification of Polysaccharides from the Immunomodulatory Medicinal Mushroom <em>Inonotus obliquus</em> |
| title_fullStr | Three-Phase Partitioning for the Extraction and Purification of Polysaccharides from the Immunomodulatory Medicinal Mushroom <em>Inonotus obliquus</em> |
| title_full_unstemmed | Three-Phase Partitioning for the Extraction and Purification of Polysaccharides from the Immunomodulatory Medicinal Mushroom <em>Inonotus obliquus</em> |
| title_short | Three-Phase Partitioning for the Extraction and Purification of Polysaccharides from the Immunomodulatory Medicinal Mushroom <em>Inonotus obliquus</em> |
| title_sort | three phase partitioning for the extraction and purification of polysaccharides from the immunomodulatory medicinal mushroom em inonotus obliquus em |
| topic | Inonotus obliquus three-phase partitioning free-radical scavenging abilities antioxidant activity immunological activity |
| url | https://www.mdpi.com/1420-3049/24/3/403 |
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