The effect of two types of diet on apoptosis indexes, lipid profile and histopathological outcome in acute kidney injury during exercise
Abstract Background Exercise and some pre-AKI diets have been shown to improve injury, apoptosis, and lipid profile. In this study, the effect of two different diets along with exercise training on acute kidney injury (AKI) was investigated. Materials and methods Laboratory rats were randomly divid...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-09-01
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| Series: | BMC Nephrology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12882-022-02938-w |
| _version_ | 1811208740325556224 |
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| author | Nazanin Sabet Zahra Soltani Mohammad Khaksari Maryam Iranpour Reza Malekpour Afshar Fatemeh Mousavi Mehdiabadi Alireza Raji-Amirhasani |
| author_facet | Nazanin Sabet Zahra Soltani Mohammad Khaksari Maryam Iranpour Reza Malekpour Afshar Fatemeh Mousavi Mehdiabadi Alireza Raji-Amirhasani |
| author_sort | Nazanin Sabet |
| collection | DOAJ |
| description | Abstract Background Exercise and some pre-AKI diets have been shown to improve injury, apoptosis, and lipid profile. In this study, the effect of two different diets along with exercise training on acute kidney injury (AKI) was investigated. Materials and methods Laboratory rats were randomly divided into four groups of control, standard diet + exercise, exercise + calorie restriction (CR) and exercise + time restriction (TR). Each group was divided into two subgroups of AKI and no AKI. The animals received endurance training and diet regimens before AKI. Fasting blood glucose, serum creatinine, Bcl-2-associated X protein (Bax), B-cell lymphoma 2 (Bcl2) and histopathological outcome of renal tissue as well as serum lipid profile of animals were assessed 24 h after AKI. Results The percentage of changes in renal Bcl2 and Bax after AKI in the group with previous exercise was lower than the group without previous exercise (p < 0.01). After induction of AKI, serum lipid profile changed in non-exercised rats (p < 0.001). Also, after injury, fasting blood glucose levels increased in non-exercised rats (p < 0.05). After injury, the start of both CR and TR diets during exercise caused less change in Bcl2 and Bax of non-exercised rats compared to exercised rats (p < 0.001). CR diet along with exercise improved lipid profile, and also CR diet along exercise decreased fasting blood glucose levels (p < 0.001). Also, both the CR and TR diets during exercise caused fewer changes in histopathological outcome after AKI. Conclusion Exercise alone decreased changes in apoptotic and histopathological indexes, fasting blood glucose, as well as lipid profile of rats after AKI. Reduction of apoptosis and improvement of histopathological outcome after AKI appeared more when CR and TR diets were commenced during exercise. The reduction of lipid profile changes was more pronounced in the group that received CR diet during exercise. |
| first_indexed | 2024-04-12T04:27:45Z |
| format | Article |
| id | doaj.art-663a84539a114e24aa747da3864a1cca |
| institution | Directory Open Access Journal |
| issn | 1471-2369 |
| language | English |
| last_indexed | 2024-04-12T04:27:45Z |
| publishDate | 2022-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Nephrology |
| spelling | doaj.art-663a84539a114e24aa747da3864a1cca2022-12-22T03:48:02ZengBMCBMC Nephrology1471-23692022-09-0123111510.1186/s12882-022-02938-wThe effect of two types of diet on apoptosis indexes, lipid profile and histopathological outcome in acute kidney injury during exerciseNazanin Sabet0Zahra Soltani1Mohammad Khaksari2Maryam Iranpour3Reza Malekpour Afshar4Fatemeh Mousavi Mehdiabadi5Alireza Raji-Amirhasani6Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical SciencesDepartment of Physiology and Pharmacology, Afzalipour Faculty of Medicine, Kerman University of Medical SciencesDepartment of Physiology and Pharmacology, Afzalipour Faculty of Medicine, Kerman University of Medical SciencesDepartment of Pathology, Pathology and Stem Cells Research Center, Afzalipour Faculty of Medicine, Kerman University of Medical SciencesDepartment of Pathology, Pathology and Stem Cells Research Center, Afzalipour Faculty of Medicine, Kerman University of Medical SciencesEndocrinology and Metabolism Research Center, Institute of Basic and Clinical Physiology Sciences, Faculty of Medicine, Kerman University of Medical SciencesDepartment of Physiology and Pharmacology, Afzalipour Faculty of Medicine, Kerman University of Medical SciencesAbstract Background Exercise and some pre-AKI diets have been shown to improve injury, apoptosis, and lipid profile. In this study, the effect of two different diets along with exercise training on acute kidney injury (AKI) was investigated. Materials and methods Laboratory rats were randomly divided into four groups of control, standard diet + exercise, exercise + calorie restriction (CR) and exercise + time restriction (TR). Each group was divided into two subgroups of AKI and no AKI. The animals received endurance training and diet regimens before AKI. Fasting blood glucose, serum creatinine, Bcl-2-associated X protein (Bax), B-cell lymphoma 2 (Bcl2) and histopathological outcome of renal tissue as well as serum lipid profile of animals were assessed 24 h after AKI. Results The percentage of changes in renal Bcl2 and Bax after AKI in the group with previous exercise was lower than the group without previous exercise (p < 0.01). After induction of AKI, serum lipid profile changed in non-exercised rats (p < 0.001). Also, after injury, fasting blood glucose levels increased in non-exercised rats (p < 0.05). After injury, the start of both CR and TR diets during exercise caused less change in Bcl2 and Bax of non-exercised rats compared to exercised rats (p < 0.001). CR diet along with exercise improved lipid profile, and also CR diet along exercise decreased fasting blood glucose levels (p < 0.001). Also, both the CR and TR diets during exercise caused fewer changes in histopathological outcome after AKI. Conclusion Exercise alone decreased changes in apoptotic and histopathological indexes, fasting blood glucose, as well as lipid profile of rats after AKI. Reduction of apoptosis and improvement of histopathological outcome after AKI appeared more when CR and TR diets were commenced during exercise. The reduction of lipid profile changes was more pronounced in the group that received CR diet during exercise.https://doi.org/10.1186/s12882-022-02938-wExerciseAcute kidney injuryApoptosisLipid profileDiet |
| spellingShingle | Nazanin Sabet Zahra Soltani Mohammad Khaksari Maryam Iranpour Reza Malekpour Afshar Fatemeh Mousavi Mehdiabadi Alireza Raji-Amirhasani The effect of two types of diet on apoptosis indexes, lipid profile and histopathological outcome in acute kidney injury during exercise BMC Nephrology Exercise Acute kidney injury Apoptosis Lipid profile Diet |
| title | The effect of two types of diet on apoptosis indexes, lipid profile and histopathological outcome in acute kidney injury during exercise |
| title_full | The effect of two types of diet on apoptosis indexes, lipid profile and histopathological outcome in acute kidney injury during exercise |
| title_fullStr | The effect of two types of diet on apoptosis indexes, lipid profile and histopathological outcome in acute kidney injury during exercise |
| title_full_unstemmed | The effect of two types of diet on apoptosis indexes, lipid profile and histopathological outcome in acute kidney injury during exercise |
| title_short | The effect of two types of diet on apoptosis indexes, lipid profile and histopathological outcome in acute kidney injury during exercise |
| title_sort | effect of two types of diet on apoptosis indexes lipid profile and histopathological outcome in acute kidney injury during exercise |
| topic | Exercise Acute kidney injury Apoptosis Lipid profile Diet |
| url | https://doi.org/10.1186/s12882-022-02938-w |
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