| Summary: | Effects of various hormonally treated substrates on mycelial development, pinning and biomass of Pleurotus ostreatus were investigated. A 3 × 4 experimental layout was used i.e. three substrates: Urochloa panicoides, Zea mays and Datura stramonium and four hormonal treatments: cytokinins; auxins; gibberellins and control. Treatments were replicated three times and arranged in a Completely Randomize Block Design. The results indicated significant differences on mycelial, pinning, fruit caps and biomass (p ≤ 0.05). Mycelial development in respective substrates was as follows: U. panicoides (100%), Z. mays (84.78%) and D. stramonium (36.31%) and pinning rate was 65.48, 32.99, and 12.23% respectively. Hormones also had significant effect on cap size and style length (p ≤ 0.05). Cap sizes were: auxins: 13.42 cm, cytokinins: 9.9 cm and gibberellins: 7.13 cm and style lengths were: 6.93, 8.83 and 11.03 cm respectively. Mushroom biomasses from different substrates were: U. panicoides (7,609.56 g), Z. mays (7,296.42 g) and D. stramonium (4,368.15 g) indicating significant influence of substrate on mushroom biomass.
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