Cervical and Vaginal Microbiomes in Early Miscarriages and Ongoing Pregnancy with and without Dydrogesterone Usage
Emerging evidence suggests that the reproductive tract microbiota is a key modulator of local inflammatory and immune pathways throughout pregnancy and may subsequently impact pregnancy outcomes. In this study, our objective was to analyze the cervical and vaginal microbiomes during early pregnancy...
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MDPI AG
2023-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/18/13836 |
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| author | Mariya Gryaznova Olesya Kozarenko Yuliya Smirnova Inna Burakova Mikhail Syromyatnikov Alexander Maslov Olga Lebedeva |
| author_facet | Mariya Gryaznova Olesya Kozarenko Yuliya Smirnova Inna Burakova Mikhail Syromyatnikov Alexander Maslov Olga Lebedeva |
| author_sort | Mariya Gryaznova |
| collection | DOAJ |
| description | Emerging evidence suggests that the reproductive tract microbiota is a key modulator of local inflammatory and immune pathways throughout pregnancy and may subsequently impact pregnancy outcomes. In this study, our objective was to analyze the cervical and vaginal microbiomes during early pregnancy among three groups: women with healthy ongoing pregnancies, women undergoing dydrogesterone treatment, and those who experienced miscarriages. The experiment involved 51 women at 8–11 weeks of gestation. The microbiome was examined using <i>16S rRNA</i> sequencing on the Ion Torrent PGM platform. Across all groups, <i>Lactobacillus iners</i> was predominant, suggesting that the vaginal community type CST III is common among the majority of participants. Notably, our data highlighted the significant roles of <i>Gardnerella vaginalis</i> and <i>Mycoplasma girerdii</i> in the pathogenesis of early miscarriage. Conversely, <i>L. iners</i> and <i>Bifidobacterium longum</i> have a protective effect in early pregnancy. Moreover, dydrogesterone intake appeared to influence notable differences between the cervical and vaginal microbiomes. Overall, our study enhanced our understanding of the cervical and vaginal microbiome composition in the eastern European population during early pregnancy. |
| first_indexed | 2024-03-10T22:41:13Z |
| format | Article |
| id | doaj.art-663d0eeab8fd4944977522bf0f260e3b |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T22:41:13Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-663d0eeab8fd4944977522bf0f260e3b2023-11-19T11:03:52ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-09-0124181383610.3390/ijms241813836Cervical and Vaginal Microbiomes in Early Miscarriages and Ongoing Pregnancy with and without Dydrogesterone UsageMariya Gryaznova0Olesya Kozarenko1Yuliya Smirnova2Inna Burakova3Mikhail Syromyatnikov4Alexander Maslov5Olga Lebedeva6Laboratory of Metagenomics and Food Biotechnology, Voronezh State University of Engineering Technologies, 394036 Voronezh, RussiaLaboratory of Metagenomics and Food Biotechnology, Voronezh State University of Engineering Technologies, 394036 Voronezh, RussiaLaboratory of Metagenomics and Food Biotechnology, Voronezh State University of Engineering Technologies, 394036 Voronezh, RussiaLaboratory of Metagenomics and Food Biotechnology, Voronezh State University of Engineering Technologies, 394036 Voronezh, RussiaLaboratory of Metagenomics and Food Biotechnology, Voronezh State University of Engineering Technologies, 394036 Voronezh, RussiaLaboratory of Metagenomics and Food Biotechnology, Voronezh State University of Engineering Technologies, 394036 Voronezh, RussiaLaboratory of Metagenomics and Food Biotechnology, Voronezh State University of Engineering Technologies, 394036 Voronezh, RussiaEmerging evidence suggests that the reproductive tract microbiota is a key modulator of local inflammatory and immune pathways throughout pregnancy and may subsequently impact pregnancy outcomes. In this study, our objective was to analyze the cervical and vaginal microbiomes during early pregnancy among three groups: women with healthy ongoing pregnancies, women undergoing dydrogesterone treatment, and those who experienced miscarriages. The experiment involved 51 women at 8–11 weeks of gestation. The microbiome was examined using <i>16S rRNA</i> sequencing on the Ion Torrent PGM platform. Across all groups, <i>Lactobacillus iners</i> was predominant, suggesting that the vaginal community type CST III is common among the majority of participants. Notably, our data highlighted the significant roles of <i>Gardnerella vaginalis</i> and <i>Mycoplasma girerdii</i> in the pathogenesis of early miscarriage. Conversely, <i>L. iners</i> and <i>Bifidobacterium longum</i> have a protective effect in early pregnancy. Moreover, dydrogesterone intake appeared to influence notable differences between the cervical and vaginal microbiomes. Overall, our study enhanced our understanding of the cervical and vaginal microbiome composition in the eastern European population during early pregnancy.https://www.mdpi.com/1422-0067/24/18/13836cervical microbiomevaginal microbiomeearly pregnancymiscarriagedydrogesterone<i>16S rRNA</i> |
| spellingShingle | Mariya Gryaznova Olesya Kozarenko Yuliya Smirnova Inna Burakova Mikhail Syromyatnikov Alexander Maslov Olga Lebedeva Cervical and Vaginal Microbiomes in Early Miscarriages and Ongoing Pregnancy with and without Dydrogesterone Usage International Journal of Molecular Sciences cervical microbiome vaginal microbiome early pregnancy miscarriage dydrogesterone <i>16S rRNA</i> |
| title | Cervical and Vaginal Microbiomes in Early Miscarriages and Ongoing Pregnancy with and without Dydrogesterone Usage |
| title_full | Cervical and Vaginal Microbiomes in Early Miscarriages and Ongoing Pregnancy with and without Dydrogesterone Usage |
| title_fullStr | Cervical and Vaginal Microbiomes in Early Miscarriages and Ongoing Pregnancy with and without Dydrogesterone Usage |
| title_full_unstemmed | Cervical and Vaginal Microbiomes in Early Miscarriages and Ongoing Pregnancy with and without Dydrogesterone Usage |
| title_short | Cervical and Vaginal Microbiomes in Early Miscarriages and Ongoing Pregnancy with and without Dydrogesterone Usage |
| title_sort | cervical and vaginal microbiomes in early miscarriages and ongoing pregnancy with and without dydrogesterone usage |
| topic | cervical microbiome vaginal microbiome early pregnancy miscarriage dydrogesterone <i>16S rRNA</i> |
| url | https://www.mdpi.com/1422-0067/24/18/13836 |
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