Inter-platform assessment of performance of high-throughput desorption electrospray ionization mass spectrometry
High throughput analysis is increasingly relevant in both industry and academia, with applications reaching from enzymology and drug discovery to organic reaction optimization and diagnostics. Currently, chromatography-free mass spectrometry (MS) techniques have acquired an important role in this fi...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-12-01
|
| Series: | Talanta Open |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666831921000163 |
| _version_ | 1818352822790389760 |
|---|---|
| author | Nicolás M. Morato R. Graham Cooks |
| author_facet | Nicolás M. Morato R. Graham Cooks |
| author_sort | Nicolás M. Morato |
| collection | DOAJ |
| description | High throughput analysis is increasingly relevant in both industry and academia, with applications reaching from enzymology and drug discovery to organic reaction optimization and diagnostics. Currently, chromatography-free mass spectrometry (MS) techniques have acquired an important role in this field due to their inherent speed, versatility, and chemical specificity. Desorption electrospray ionization (DESI) MS is one of these approaches, which allows for direct analysis of complex samples in the ambient environment, with throughputs better than 1 sample per second and no need for sample treatment. Here we assess the evolution of the high throughput DESI-MS analytical performance from the early DESI source developed by Prosolia Inc. to the recently commercialized version of Waters Corporation. The evaluation was carried out through both quantitative and qualitative analysis of biologically relevant compounds including metabolites, peptides, lipids and pharmaceuticals. Our results indicate that both platforms are successful at the direct analysis of these species even from complex matrices such as bioassay buffers. However, the newest iteration of the DESI stage, when combined with a quadrupole time-of-flight (Q-ToF) instrument, provides higher sensitivity (1-3 orders of magnitude lower limits of detection) and reproducibility (ca. 10% average reduction in coefficients of variation for quantitation using an internal standard). The cases explored in this study also showcase the broad applicability of high throughput DESI-MS for bioanalysis and quality control. |
| first_indexed | 2024-12-13T18:59:45Z |
| format | Article |
| id | doaj.art-663dfcedd0014b608f7e777bc9847bc1 |
| institution | Directory Open Access Journal |
| issn | 2666-8319 |
| language | English |
| last_indexed | 2024-12-13T18:59:45Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Talanta Open |
| spelling | doaj.art-663dfcedd0014b608f7e777bc9847bc12022-12-21T23:34:43ZengElsevierTalanta Open2666-83192021-12-014100046Inter-platform assessment of performance of high-throughput desorption electrospray ionization mass spectrometryNicolás M. Morato0R. Graham Cooks1Department of Chemistry and Bindley Bioscience Center, Purdue University, West Lafayette, IN 47907, USACorresponding author: Department of Chemistry, Purdue University, 560 Oval Drive, West Lafayette, IN 47907, USA; Department of Chemistry and Bindley Bioscience Center, Purdue University, West Lafayette, IN 47907, USAHigh throughput analysis is increasingly relevant in both industry and academia, with applications reaching from enzymology and drug discovery to organic reaction optimization and diagnostics. Currently, chromatography-free mass spectrometry (MS) techniques have acquired an important role in this field due to their inherent speed, versatility, and chemical specificity. Desorption electrospray ionization (DESI) MS is one of these approaches, which allows for direct analysis of complex samples in the ambient environment, with throughputs better than 1 sample per second and no need for sample treatment. Here we assess the evolution of the high throughput DESI-MS analytical performance from the early DESI source developed by Prosolia Inc. to the recently commercialized version of Waters Corporation. The evaluation was carried out through both quantitative and qualitative analysis of biologically relevant compounds including metabolites, peptides, lipids and pharmaceuticals. Our results indicate that both platforms are successful at the direct analysis of these species even from complex matrices such as bioassay buffers. However, the newest iteration of the DESI stage, when combined with a quadrupole time-of-flight (Q-ToF) instrument, provides higher sensitivity (1-3 orders of magnitude lower limits of detection) and reproducibility (ca. 10% average reduction in coefficients of variation for quantitation using an internal standard). The cases explored in this study also showcase the broad applicability of high throughput DESI-MS for bioanalysis and quality control.http://www.sciencedirect.com/science/article/pii/S2666831921000163analytical performanceambient ionizationsteroid sulfatesopioid peptideslipidscompound library |
| spellingShingle | Nicolás M. Morato R. Graham Cooks Inter-platform assessment of performance of high-throughput desorption electrospray ionization mass spectrometry Talanta Open analytical performance ambient ionization steroid sulfates opioid peptides lipids compound library |
| title | Inter-platform assessment of performance of high-throughput desorption electrospray ionization mass spectrometry |
| title_full | Inter-platform assessment of performance of high-throughput desorption electrospray ionization mass spectrometry |
| title_fullStr | Inter-platform assessment of performance of high-throughput desorption electrospray ionization mass spectrometry |
| title_full_unstemmed | Inter-platform assessment of performance of high-throughput desorption electrospray ionization mass spectrometry |
| title_short | Inter-platform assessment of performance of high-throughput desorption electrospray ionization mass spectrometry |
| title_sort | inter platform assessment of performance of high throughput desorption electrospray ionization mass spectrometry |
| topic | analytical performance ambient ionization steroid sulfates opioid peptides lipids compound library |
| url | http://www.sciencedirect.com/science/article/pii/S2666831921000163 |
| work_keys_str_mv | AT nicolasmmorato interplatformassessmentofperformanceofhighthroughputdesorptionelectrosprayionizationmassspectrometry AT rgrahamcooks interplatformassessmentofperformanceofhighthroughputdesorptionelectrosprayionizationmassspectrometry |