Effects of Compound Probiotics on Cecal Microbiome and Metabolome of Shaoxing Duck
This experiment was conducted to investigate the effects of compound probiotics on intestinal microflora and metabolome of Shaoxing ducks. A total of 640 1-day-old Shaoxing ducks were randomly divided into two treatments with eight replicates and forty ducks for each replicate. The ducks were fed ba...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-01-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.813598/full |
| _version_ | 1818943315100303360 |
|---|---|
| author | Hanxue Sun Hanxue Sun Xizhong Du Tao Zeng Shenggang Ruan Guoqin Li Zhengrong Tao Wenwu Xu Lizhi Lu |
| author_facet | Hanxue Sun Hanxue Sun Xizhong Du Tao Zeng Shenggang Ruan Guoqin Li Zhengrong Tao Wenwu Xu Lizhi Lu |
| author_sort | Hanxue Sun |
| collection | DOAJ |
| description | This experiment was conducted to investigate the effects of compound probiotics on intestinal microflora and metabolome of Shaoxing ducks. A total of 640 1-day-old Shaoxing ducks were randomly divided into two treatments with eight replicates and forty ducks for each replicate. The ducks were fed basal diet (Ctrl) and basal diet supplemented with 0.15% compound probiotics (MixP). The experiment lasted for 85 days. The results showed that the abundance of Bacteroidetes and Bacteroides in MixP was higher than that in Ctrl (P < 0.05). However, the abundance of Firmicutes and Oscillospira and Desulfovibrio in MixP was lower than that in Ctrl (P < 0.05). Concentrations of 71 metabolites differed significantly (P < 0.05) between the MixP and the Ctrl groups; for example, Pyridoxal (Vitamin B6), L-Arginine, and Betaine aldehyde were up-regulated (P < 0.05), and 7-oxocholesterol, 3-hydroxy-L-kynureni-ne, and N-acetyl-d-glucosamine were down-regulated (P < 0.05). KEGG was enriched in 15 metabolic pathways. The pathways of Vitamin B6 metabolism, Vascular smooth muscle contraction, Vitamin digestion and absorption, and Protein digestion and absorption were influenced by compound probiotics supplementation. Thus, supplementation of compound probiotics improved cecal heath through shifts in the cecal microbiome and metabolome. |
| first_indexed | 2024-12-20T07:25:22Z |
| format | Article |
| id | doaj.art-6640c8c367f04f8eaffc563d06573508 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-20T07:25:22Z |
| publishDate | 2022-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-6640c8c367f04f8eaffc563d065735082022-12-21T19:48:33ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-01-011210.3389/fmicb.2021.813598813598Effects of Compound Probiotics on Cecal Microbiome and Metabolome of Shaoxing DuckHanxue Sun0Hanxue Sun1Xizhong Du2Tao Zeng3Shenggang Ruan4Guoqin Li5Zhengrong Tao6Wenwu Xu7Lizhi Lu8Animal Husbandry and Veterinary Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei, ChinaJinhua Academy of Agricultural Sciences, Jinhua, ChinaAnimal Husbandry and Veterinary Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaShaoxing Xianheng Shao Duck Breeding Co., Ltd., Shaoxing, ChinaAnimal Husbandry and Veterinary Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaAnimal Husbandry and Veterinary Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaAnimal Husbandry and Veterinary Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaAnimal Husbandry and Veterinary Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaThis experiment was conducted to investigate the effects of compound probiotics on intestinal microflora and metabolome of Shaoxing ducks. A total of 640 1-day-old Shaoxing ducks were randomly divided into two treatments with eight replicates and forty ducks for each replicate. The ducks were fed basal diet (Ctrl) and basal diet supplemented with 0.15% compound probiotics (MixP). The experiment lasted for 85 days. The results showed that the abundance of Bacteroidetes and Bacteroides in MixP was higher than that in Ctrl (P < 0.05). However, the abundance of Firmicutes and Oscillospira and Desulfovibrio in MixP was lower than that in Ctrl (P < 0.05). Concentrations of 71 metabolites differed significantly (P < 0.05) between the MixP and the Ctrl groups; for example, Pyridoxal (Vitamin B6), L-Arginine, and Betaine aldehyde were up-regulated (P < 0.05), and 7-oxocholesterol, 3-hydroxy-L-kynureni-ne, and N-acetyl-d-glucosamine were down-regulated (P < 0.05). KEGG was enriched in 15 metabolic pathways. The pathways of Vitamin B6 metabolism, Vascular smooth muscle contraction, Vitamin digestion and absorption, and Protein digestion and absorption were influenced by compound probiotics supplementation. Thus, supplementation of compound probiotics improved cecal heath through shifts in the cecal microbiome and metabolome.https://www.frontiersin.org/articles/10.3389/fmicb.2021.813598/fullcompound probioticsduckintestinal microflora16S rRNAmetabolome |
| spellingShingle | Hanxue Sun Hanxue Sun Xizhong Du Tao Zeng Shenggang Ruan Guoqin Li Zhengrong Tao Wenwu Xu Lizhi Lu Effects of Compound Probiotics on Cecal Microbiome and Metabolome of Shaoxing Duck Frontiers in Microbiology compound probiotics duck intestinal microflora 16S rRNA metabolome |
| title | Effects of Compound Probiotics on Cecal Microbiome and Metabolome of Shaoxing Duck |
| title_full | Effects of Compound Probiotics on Cecal Microbiome and Metabolome of Shaoxing Duck |
| title_fullStr | Effects of Compound Probiotics on Cecal Microbiome and Metabolome of Shaoxing Duck |
| title_full_unstemmed | Effects of Compound Probiotics on Cecal Microbiome and Metabolome of Shaoxing Duck |
| title_short | Effects of Compound Probiotics on Cecal Microbiome and Metabolome of Shaoxing Duck |
| title_sort | effects of compound probiotics on cecal microbiome and metabolome of shaoxing duck |
| topic | compound probiotics duck intestinal microflora 16S rRNA metabolome |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.813598/full |
| work_keys_str_mv | AT hanxuesun effectsofcompoundprobioticsoncecalmicrobiomeandmetabolomeofshaoxingduck AT hanxuesun effectsofcompoundprobioticsoncecalmicrobiomeandmetabolomeofshaoxingduck AT xizhongdu effectsofcompoundprobioticsoncecalmicrobiomeandmetabolomeofshaoxingduck AT taozeng effectsofcompoundprobioticsoncecalmicrobiomeandmetabolomeofshaoxingduck AT shenggangruan effectsofcompoundprobioticsoncecalmicrobiomeandmetabolomeofshaoxingduck AT guoqinli effectsofcompoundprobioticsoncecalmicrobiomeandmetabolomeofshaoxingduck AT zhengrongtao effectsofcompoundprobioticsoncecalmicrobiomeandmetabolomeofshaoxingduck AT wenwuxu effectsofcompoundprobioticsoncecalmicrobiomeandmetabolomeofshaoxingduck AT lizhilu effectsofcompoundprobioticsoncecalmicrobiomeandmetabolomeofshaoxingduck |