INFLUENCE OF FIBRIN D AND DD FRAGMENTS ON FIBRINOGEN AND FIBRINOGEN FRAGMENT X POLYMERIZATION INITIATED BY THROMBIN OR ANCISTRON
Aim. Study of the role of the complex between the ?C region and the B?N domain in the initial stages of fibrin polymerization has been investigated. Materials and Methods. Method of turbidimetry to study the influence of fibrinogen fragments D and DD on the polymerization and methods of isolation...
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| Format: | Article |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2022-06-01
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| Series: | Biotechnologia Acta |
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| Online Access: | http://biotechnology.kiev.ua/images/3_2022/tsap_3_2022.pdf |
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| author | Tsap P. Yu. Gogolinskaia G. K. Platonova T. M. Marunych R. Yu. Udovenko A. V., Makogonenko, Ye. M. |
| author_facet | Tsap P. Yu. Gogolinskaia G. K. Platonova T. M. Marunych R. Yu. Udovenko A. V., Makogonenko, Ye. M. |
| author_sort | Tsap P. Yu. |
| collection | DOAJ |
| description | Aim. Study of the role of the complex between the ?C region and the B?N domain in the initial stages of fibrin polymerization has been investigated.
Materials and Methods. Method of turbidimetry to study the influence of fibrinogen fragments D and DD on the polymerization and methods of isolation, purification, fragmentation for fibrinogen, monomer and cross-linked fibrin, fibrinogen X-fragment, Glu -plasminogen were used.
Results. It was shown that fragment DD completely inhibited polymerization process in all the systems examined (“Fg + Thr”, “Fg + Anc H”, “X + Thr”, “X + Anc H”). Fragment D inhibited fibrin polymerization at all stages in the system “Fg + Thr”, but in the system “Fg + Anc H” it almost did not influence fibrin polymerization. In the both systems “X + Thr” and “X + Anc H” fragment D weakly inhibited the self-assembly of fibrin molecules into protofibrils, but accelerated the process of lateral association in the second system.
Conclusions. The data obtained indicated that the complex between the ?C region and the B?N domain of fibrin desA, on the initial stage of polymerization supported the rate of self-assembling and lateral association of fibrin desA protofibrils, protecting the oligomers against the depolymerizing influence of fibrinogen. |
| first_indexed | 2024-03-12T05:35:27Z |
| format | Article |
| id | doaj.art-6642ca18177c4f9d989a25ac7102084b |
| institution | Directory Open Access Journal |
| issn | 2410-7751 2410-776X |
| language | English |
| last_indexed | 2024-03-12T05:35:27Z |
| publishDate | 2022-06-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | Biotechnologia Acta |
| spelling | doaj.art-6642ca18177c4f9d989a25ac7102084b2023-09-03T06:27:45ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryBiotechnologia Acta2410-77512410-776X2022-06-01153232810.15407/biotech15.03.023INFLUENCE OF FIBRIN D AND DD FRAGMENTS ON FIBRINOGEN AND FIBRINOGEN FRAGMENT X POLYMERIZATION INITIATED BY THROMBIN OR ANCISTRONTsap P. Yu.0 Gogolinskaia G. K.1Platonova T. M.2Marunych R. Yu.3Udovenko A. V., 4Makogonenko, Ye. M.5Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, KyivPalladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, KyivPalladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, KyivPalladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, KyivPalladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, KyivPalladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, KyivAim. Study of the role of the complex between the ?C region and the B?N domain in the initial stages of fibrin polymerization has been investigated. Materials and Methods. Method of turbidimetry to study the influence of fibrinogen fragments D and DD on the polymerization and methods of isolation, purification, fragmentation for fibrinogen, monomer and cross-linked fibrin, fibrinogen X-fragment, Glu -plasminogen were used. Results. It was shown that fragment DD completely inhibited polymerization process in all the systems examined (“Fg + Thr”, “Fg + Anc H”, “X + Thr”, “X + Anc H”). Fragment D inhibited fibrin polymerization at all stages in the system “Fg + Thr”, but in the system “Fg + Anc H” it almost did not influence fibrin polymerization. In the both systems “X + Thr” and “X + Anc H” fragment D weakly inhibited the self-assembly of fibrin molecules into protofibrils, but accelerated the process of lateral association in the second system. Conclusions. The data obtained indicated that the complex between the ?C region and the B?N domain of fibrin desA, on the initial stage of polymerization supported the rate of self-assembling and lateral association of fibrin desA protofibrils, protecting the oligomers against the depolymerizing influence of fibrinogen.http://biotechnology.kiev.ua/images/3_2022/tsap_3_2022.pdffibrinogenfibrinfragment ddd dimerfragment xb?n-domain-?c-region complex. |
| spellingShingle | Tsap P. Yu. Gogolinskaia G. K. Platonova T. M. Marunych R. Yu. Udovenko A. V., Makogonenko, Ye. M. INFLUENCE OF FIBRIN D AND DD FRAGMENTS ON FIBRINOGEN AND FIBRINOGEN FRAGMENT X POLYMERIZATION INITIATED BY THROMBIN OR ANCISTRON Biotechnologia Acta fibrinogen fibrin fragment dd d dimer fragment x b?n-domain-?c-region complex. |
| title | INFLUENCE OF FIBRIN D AND DD FRAGMENTS ON FIBRINOGEN AND FIBRINOGEN FRAGMENT X POLYMERIZATION INITIATED BY THROMBIN OR ANCISTRON |
| title_full | INFLUENCE OF FIBRIN D AND DD FRAGMENTS ON FIBRINOGEN AND FIBRINOGEN FRAGMENT X POLYMERIZATION INITIATED BY THROMBIN OR ANCISTRON |
| title_fullStr | INFLUENCE OF FIBRIN D AND DD FRAGMENTS ON FIBRINOGEN AND FIBRINOGEN FRAGMENT X POLYMERIZATION INITIATED BY THROMBIN OR ANCISTRON |
| title_full_unstemmed | INFLUENCE OF FIBRIN D AND DD FRAGMENTS ON FIBRINOGEN AND FIBRINOGEN FRAGMENT X POLYMERIZATION INITIATED BY THROMBIN OR ANCISTRON |
| title_short | INFLUENCE OF FIBRIN D AND DD FRAGMENTS ON FIBRINOGEN AND FIBRINOGEN FRAGMENT X POLYMERIZATION INITIATED BY THROMBIN OR ANCISTRON |
| title_sort | influence of fibrin d and dd fragments on fibrinogen and fibrinogen fragment x polymerization initiated by thrombin or ancistron |
| topic | fibrinogen fibrin fragment dd d dimer fragment x b?n-domain-?c-region complex. |
| url | http://biotechnology.kiev.ua/images/3_2022/tsap_3_2022.pdf |
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