Differential protein expression in seminal plasma from fertile and infertile males
Aim: The aim of this study was to analyze human seminal plasma proteins in association with male fertility status using the proteomic mass spectrometry technology Surface-Enhanced Laser Desorption Ionization Time-of-Flight (SELDI-TOF-MS). Materials and Methods: Semen analysis was performed using con...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2014-01-01
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| Series: | Journal of Human Reproductive Sciences |
| Subjects: | |
| Online Access: | http://www.jhrsonline.org/article.asp?issn=0974-1208;year=2014;volume=7;issue=3;spage=206;epage=211;aulast=Cadavid |
| _version_ | 1798045384021377024 |
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| author | Angela P Cadavid J Angela Alvarez Udo R Markert Walter Cardona Maya |
| author_facet | Angela P Cadavid J Angela Alvarez Udo R Markert Walter Cardona Maya |
| author_sort | Angela P Cadavid J |
| collection | DOAJ |
| description | Aim: The aim of this study was to analyze human seminal plasma proteins in association with male fertility status using the proteomic mass spectrometry technology Surface-Enhanced Laser Desorption Ionization Time-of-Flight (SELDI-TOF-MS). Materials and Methods: Semen analysis was performed using conventional methods. Protein profiles of the seminal plasma were obtained by SELDI-TOF mass spectrometry over a strong anion exchanger, ProteinChip® Q10 array. Results and Conclusion: We found statistically significant differences in motility and sperm count between fertile and infertile men. In addition, we observed ten seminal proteins that are significantly up-regulated in the infertile group. In conclusion, comparison of seminal plasma proteome in fertile and infertile men provides new aspects in the physiology of male fertility and might help in identifying novel markers of male infertility. |
| first_indexed | 2024-04-11T23:20:00Z |
| format | Article |
| id | doaj.art-6643e5d7fd194662996a6b18d27d69d0 |
| institution | Directory Open Access Journal |
| issn | 0974-1208 1998-4766 |
| language | English |
| last_indexed | 2024-04-11T23:20:00Z |
| publishDate | 2014-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Human Reproductive Sciences |
| spelling | doaj.art-6643e5d7fd194662996a6b18d27d69d02022-12-22T03:57:29ZengWolters Kluwer Medknow PublicationsJournal of Human Reproductive Sciences0974-12081998-47662014-01-017320621110.4103/0974-1208.142485Differential protein expression in seminal plasma from fertile and infertile malesAngela P Cadavid JAngela AlvarezUdo R MarkertWalter Cardona MayaAim: The aim of this study was to analyze human seminal plasma proteins in association with male fertility status using the proteomic mass spectrometry technology Surface-Enhanced Laser Desorption Ionization Time-of-Flight (SELDI-TOF-MS). Materials and Methods: Semen analysis was performed using conventional methods. Protein profiles of the seminal plasma were obtained by SELDI-TOF mass spectrometry over a strong anion exchanger, ProteinChip® Q10 array. Results and Conclusion: We found statistically significant differences in motility and sperm count between fertile and infertile men. In addition, we observed ten seminal proteins that are significantly up-regulated in the infertile group. In conclusion, comparison of seminal plasma proteome in fertile and infertile men provides new aspects in the physiology of male fertility and might help in identifying novel markers of male infertility.http://www.jhrsonline.org/article.asp?issn=0974-1208;year=2014;volume=7;issue=3;spage=206;epage=211;aulast=CadavidFertilityproteomicsseminal plasmasurface-enhanced laser desorption ionization time-of-flight |
| spellingShingle | Angela P Cadavid J Angela Alvarez Udo R Markert Walter Cardona Maya Differential protein expression in seminal plasma from fertile and infertile males Journal of Human Reproductive Sciences Fertility proteomics seminal plasma surface-enhanced laser desorption ionization time-of-flight |
| title | Differential protein expression in seminal plasma from fertile and infertile males |
| title_full | Differential protein expression in seminal plasma from fertile and infertile males |
| title_fullStr | Differential protein expression in seminal plasma from fertile and infertile males |
| title_full_unstemmed | Differential protein expression in seminal plasma from fertile and infertile males |
| title_short | Differential protein expression in seminal plasma from fertile and infertile males |
| title_sort | differential protein expression in seminal plasma from fertile and infertile males |
| topic | Fertility proteomics seminal plasma surface-enhanced laser desorption ionization time-of-flight |
| url | http://www.jhrsonline.org/article.asp?issn=0974-1208;year=2014;volume=7;issue=3;spage=206;epage=211;aulast=Cadavid |
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