Gender difference in hepatic AMPK pathway activated lipid metabolism induced by aged polystyrene microplastics exposure
Microplastics (MPs) pollution becomes an increasing concern and researchers keep exploring the health effects caused by MPs exposure. The ageing process in the environment significantly alters the physicochemical characteristics of MPs and subsequently affects their toxicities. The health effects of...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-10-01
|
| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651322009459 |
| _version_ | 1828170544101457920 |
|---|---|
| author | Xiaona Yang Jin Jiang Qing Wang Jiawei Duan Na Chen Di Wu Yankai Xia |
| author_facet | Xiaona Yang Jin Jiang Qing Wang Jiawei Duan Na Chen Di Wu Yankai Xia |
| author_sort | Xiaona Yang |
| collection | DOAJ |
| description | Microplastics (MPs) pollution becomes an increasing concern and researchers keep exploring the health effects caused by MPs exposure. The ageing process in the environment significantly alters the physicochemical characteristics of MPs and subsequently affects their toxicities. The health effects of aged MPs exposure and the mechanism underlying are worthy of exploration. Polystyrene microplastics (PS-MPs) (with size less than 50 µm) were obtained by grinding and screening polystyrene materials. PS-MPs continued to be aged by ozone treatment (0.4 mg/min, 9 h). Both male and female C57BL/6 mice were orally exposed to 0 or 2 mg/kg/d aged PS-MPs for 28 days. Results showed that PS-MPs were found in liver, ovary and spleen of females and liver, testis and spleen of males in the aged PS-MPs group. Exposure to aged PS-MPs significantly decreased abdominal fat/body coefficient, the adipocyte size and the serum LDL-C level in females. Compared to the control, serum estradiol (E2) level, the mRNA expression levels of genes regulating E2 production (17β-hsd, 3β-hsd and Star) in ovary and the protein expression levels of E2 receptors (ERα, ERβ), AMPKα and p-AMPKα1 in liver increased significantly, and the mRNA expression levels of AMP-activated protein kinase (AMPK) downstream genes (Srebp-1c, Fas and Scd1) in liver decreased significantly in the female aged PS-MPs group. Liver metabolomic profiling showed that differential metabolites between female aged PS-MPs group and female control group were enriched in biotin metabolism and the level of biotin increased significantly in the female aged PS-MPs group. However, no significant changes were detected in males. These results indicated that aged PS-MPs exposure increased ovarian E2 production and activated the AMPK pathway in the liver which might inhibit liver lipid synthesis only in females. Our findings provide new insights into the potential sex-specific health effects of environmental MPs pollution. |
| first_indexed | 2024-04-12T03:12:14Z |
| format | Article |
| id | doaj.art-6647fa496efe415c9703dd6f20e5ae8f |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-12T03:12:14Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-6647fa496efe415c9703dd6f20e5ae8f2022-12-22T03:50:19ZengElsevierEcotoxicology and Environmental Safety0147-65132022-10-01245114105Gender difference in hepatic AMPK pathway activated lipid metabolism induced by aged polystyrene microplastics exposureXiaona Yang0Jin Jiang1Qing Wang2Jiawei Duan3Na Chen4Di Wu5Yankai Xia6State Key Laboratory of Reproductive Medicine, Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing 211166, China; Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, ChinaState Key Laboratory of Reproductive Medicine, Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing 211166, China; Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, ChinaState Key Laboratory of Reproductive Medicine, Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing 211166, China; Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, ChinaState Key Laboratory of Reproductive Medicine, Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing 211166, China; Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, ChinaState Key Laboratory of Reproductive Medicine, Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing 211166, China; Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, ChinaState Key Laboratory of Reproductive Medicine, Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing 211166, China; Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, China; Correspondence to: State Key Laboratory of Reproductive Medicine, School of Public Health, Nanjing Medical University, Nanjing 211166, China.State Key Laboratory of Reproductive Medicine, Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing 211166, China; Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, ChinaMicroplastics (MPs) pollution becomes an increasing concern and researchers keep exploring the health effects caused by MPs exposure. The ageing process in the environment significantly alters the physicochemical characteristics of MPs and subsequently affects their toxicities. The health effects of aged MPs exposure and the mechanism underlying are worthy of exploration. Polystyrene microplastics (PS-MPs) (with size less than 50 µm) were obtained by grinding and screening polystyrene materials. PS-MPs continued to be aged by ozone treatment (0.4 mg/min, 9 h). Both male and female C57BL/6 mice were orally exposed to 0 or 2 mg/kg/d aged PS-MPs for 28 days. Results showed that PS-MPs were found in liver, ovary and spleen of females and liver, testis and spleen of males in the aged PS-MPs group. Exposure to aged PS-MPs significantly decreased abdominal fat/body coefficient, the adipocyte size and the serum LDL-C level in females. Compared to the control, serum estradiol (E2) level, the mRNA expression levels of genes regulating E2 production (17β-hsd, 3β-hsd and Star) in ovary and the protein expression levels of E2 receptors (ERα, ERβ), AMPKα and p-AMPKα1 in liver increased significantly, and the mRNA expression levels of AMP-activated protein kinase (AMPK) downstream genes (Srebp-1c, Fas and Scd1) in liver decreased significantly in the female aged PS-MPs group. Liver metabolomic profiling showed that differential metabolites between female aged PS-MPs group and female control group were enriched in biotin metabolism and the level of biotin increased significantly in the female aged PS-MPs group. However, no significant changes were detected in males. These results indicated that aged PS-MPs exposure increased ovarian E2 production and activated the AMPK pathway in the liver which might inhibit liver lipid synthesis only in females. Our findings provide new insights into the potential sex-specific health effects of environmental MPs pollution.http://www.sciencedirect.com/science/article/pii/S0147651322009459Polystyrene microplasticsEstradiolAMPKLipid metabolismLiverOvary |
| spellingShingle | Xiaona Yang Jin Jiang Qing Wang Jiawei Duan Na Chen Di Wu Yankai Xia Gender difference in hepatic AMPK pathway activated lipid metabolism induced by aged polystyrene microplastics exposure Ecotoxicology and Environmental Safety Polystyrene microplastics Estradiol AMPK Lipid metabolism Liver Ovary |
| title | Gender difference in hepatic AMPK pathway activated lipid metabolism induced by aged polystyrene microplastics exposure |
| title_full | Gender difference in hepatic AMPK pathway activated lipid metabolism induced by aged polystyrene microplastics exposure |
| title_fullStr | Gender difference in hepatic AMPK pathway activated lipid metabolism induced by aged polystyrene microplastics exposure |
| title_full_unstemmed | Gender difference in hepatic AMPK pathway activated lipid metabolism induced by aged polystyrene microplastics exposure |
| title_short | Gender difference in hepatic AMPK pathway activated lipid metabolism induced by aged polystyrene microplastics exposure |
| title_sort | gender difference in hepatic ampk pathway activated lipid metabolism induced by aged polystyrene microplastics exposure |
| topic | Polystyrene microplastics Estradiol AMPK Lipid metabolism Liver Ovary |
| url | http://www.sciencedirect.com/science/article/pii/S0147651322009459 |
| work_keys_str_mv | AT xiaonayang genderdifferenceinhepaticampkpathwayactivatedlipidmetabolisminducedbyagedpolystyrenemicroplasticsexposure AT jinjiang genderdifferenceinhepaticampkpathwayactivatedlipidmetabolisminducedbyagedpolystyrenemicroplasticsexposure AT qingwang genderdifferenceinhepaticampkpathwayactivatedlipidmetabolisminducedbyagedpolystyrenemicroplasticsexposure AT jiaweiduan genderdifferenceinhepaticampkpathwayactivatedlipidmetabolisminducedbyagedpolystyrenemicroplasticsexposure AT nachen genderdifferenceinhepaticampkpathwayactivatedlipidmetabolisminducedbyagedpolystyrenemicroplasticsexposure AT diwu genderdifferenceinhepaticampkpathwayactivatedlipidmetabolisminducedbyagedpolystyrenemicroplasticsexposure AT yankaixia genderdifferenceinhepaticampkpathwayactivatedlipidmetabolisminducedbyagedpolystyrenemicroplasticsexposure |