N6-(2-hydroxyethyl)-Adenosine Induces Apoptosis via ER Stress and Autophagy of Gastric Carcinoma Cells In Vitro and In Vivo
Gastric cancer is the most common malignant tumor of the digestive tract and is great challenge in clinical treatment. N6-(2-Hydroxyethyl)-adenosine (HEA), widely present in various fungi, is a natural adenosine derivative with many biological and pharmacological activities. Here, we assessed the an...
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MDPI AG
2020-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/16/5815 |
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| author | Hongqing Xie Xiaotong Li Weiwei Yang Liping Yu Xiasen Jiang Yajie Chen Zhangfei Shen Conghui Li Meier Gu Liangen Shi |
| author_facet | Hongqing Xie Xiaotong Li Weiwei Yang Liping Yu Xiasen Jiang Yajie Chen Zhangfei Shen Conghui Li Meier Gu Liangen Shi |
| author_sort | Hongqing Xie |
| collection | DOAJ |
| description | Gastric cancer is the most common malignant tumor of the digestive tract and is great challenge in clinical treatment. N6-(2-Hydroxyethyl)-adenosine (HEA), widely present in various fungi, is a natural adenosine derivative with many biological and pharmacological activities. Here, we assessed the antineoplastic effect of HEA on gastric carcinoma. HEA exerted cytotoxic effects against gastric carcinoma cells (SGC-7901 and AGS) in a dose and time-dependent manner. Additionally, we found that HEA induced reactive oxygen species production and mitochondrial membrane potential depolarization. Moreover, it could trigger caspase-dependent apoptosis, promoting intracellular Ca<sup>2+</sup>-related endoplasmic reticulum (ER) stress and autophagy. On the other hand, HEA could significantly inhibit the growth of transplanted tumors in nude mice and induce apoptosis of tumor tissues cells in vivo. In conclusion, HEA induced apoptosis of gastric carcinoma cells in vitro and in vivo, demonstrating that HEA is a potential chemotherapeutic agent for gastric carcinoma. |
| first_indexed | 2024-03-10T17:30:39Z |
| format | Article |
| id | doaj.art-6651cc834d9748e2a81766c2ec31efb0 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T17:30:39Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-6651cc834d9748e2a81766c2ec31efb02023-11-20T10:02:27ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-08-012116581510.3390/ijms21165815N6-(2-hydroxyethyl)-Adenosine Induces Apoptosis via ER Stress and Autophagy of Gastric Carcinoma Cells In Vitro and In VivoHongqing Xie0Xiaotong Li1Weiwei Yang2Liping Yu3Xiasen Jiang4Yajie Chen5Zhangfei Shen6Conghui Li7Meier Gu8Liangen Shi9College of Animal Sciences, Zhejiang University, Hangzhou 310058, ChinaCollege of Animal Sciences, Zhejiang University, Hangzhou 310058, ChinaLaboratory Animal Center, Hangzhou Normal University, Hangzhou 311121, ChinaLaboratory Animal Center, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Animal Sciences, Zhejiang University, Hangzhou 310058, ChinaCollege of Animal Sciences, Zhejiang University, Hangzhou 310058, ChinaCollege of Animal Sciences, Zhejiang University, Hangzhou 310058, ChinaCollege of Animal Sciences, Zhejiang University, Hangzhou 310058, ChinaLaboratory Animal Center, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Animal Sciences, Zhejiang University, Hangzhou 310058, ChinaGastric cancer is the most common malignant tumor of the digestive tract and is great challenge in clinical treatment. N6-(2-Hydroxyethyl)-adenosine (HEA), widely present in various fungi, is a natural adenosine derivative with many biological and pharmacological activities. Here, we assessed the antineoplastic effect of HEA on gastric carcinoma. HEA exerted cytotoxic effects against gastric carcinoma cells (SGC-7901 and AGS) in a dose and time-dependent manner. Additionally, we found that HEA induced reactive oxygen species production and mitochondrial membrane potential depolarization. Moreover, it could trigger caspase-dependent apoptosis, promoting intracellular Ca<sup>2+</sup>-related endoplasmic reticulum (ER) stress and autophagy. On the other hand, HEA could significantly inhibit the growth of transplanted tumors in nude mice and induce apoptosis of tumor tissues cells in vivo. In conclusion, HEA induced apoptosis of gastric carcinoma cells in vitro and in vivo, demonstrating that HEA is a potential chemotherapeutic agent for gastric carcinoma.https://www.mdpi.com/1422-0067/21/16/5815N6-(2-Hydroxyethyl)-adenosineapoptosisendoplasmic reticulum stressgastric carcinomaautophagy |
| spellingShingle | Hongqing Xie Xiaotong Li Weiwei Yang Liping Yu Xiasen Jiang Yajie Chen Zhangfei Shen Conghui Li Meier Gu Liangen Shi N6-(2-hydroxyethyl)-Adenosine Induces Apoptosis via ER Stress and Autophagy of Gastric Carcinoma Cells In Vitro and In Vivo International Journal of Molecular Sciences N6-(2-Hydroxyethyl)-adenosine apoptosis endoplasmic reticulum stress gastric carcinoma autophagy |
| title | N6-(2-hydroxyethyl)-Adenosine Induces Apoptosis via ER Stress and Autophagy of Gastric Carcinoma Cells In Vitro and In Vivo |
| title_full | N6-(2-hydroxyethyl)-Adenosine Induces Apoptosis via ER Stress and Autophagy of Gastric Carcinoma Cells In Vitro and In Vivo |
| title_fullStr | N6-(2-hydroxyethyl)-Adenosine Induces Apoptosis via ER Stress and Autophagy of Gastric Carcinoma Cells In Vitro and In Vivo |
| title_full_unstemmed | N6-(2-hydroxyethyl)-Adenosine Induces Apoptosis via ER Stress and Autophagy of Gastric Carcinoma Cells In Vitro and In Vivo |
| title_short | N6-(2-hydroxyethyl)-Adenosine Induces Apoptosis via ER Stress and Autophagy of Gastric Carcinoma Cells In Vitro and In Vivo |
| title_sort | n6 2 hydroxyethyl adenosine induces apoptosis via er stress and autophagy of gastric carcinoma cells in vitro and in vivo |
| topic | N6-(2-Hydroxyethyl)-adenosine apoptosis endoplasmic reticulum stress gastric carcinoma autophagy |
| url | https://www.mdpi.com/1422-0067/21/16/5815 |
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