Facile Label-Free Electrochemical DNA Biosensor for Detection of Osteosarcoma-Related Survivin Gene
A sensitive and selective electrochemical deoxyribonucleic acid (DNA) biosensor was developed for the determination of a osteosarcoma-related survivin gene by using celestine blue (CB) as a label-free hybridization indicator. The proposed strategy adopted a facile and low-cost working electrode with...
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MDPI AG
2022-09-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/9/747 |
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| author | Yao Chen Yu Zhong Ji-Xing Ye Yun Lei Ai-Lin Liu |
| author_facet | Yao Chen Yu Zhong Ji-Xing Ye Yun Lei Ai-Lin Liu |
| author_sort | Yao Chen |
| collection | DOAJ |
| description | A sensitive and selective electrochemical deoxyribonucleic acid (DNA) biosensor was developed for the determination of a osteosarcoma-related survivin gene by using celestine blue (CB) as a label-free hybridization indicator. The proposed strategy adopted a facile and low-cost working electrode with no need for other substances for electrode or DNA functionalization. The interaction mode between CB and DNA was studied by electrochemical and spectroscopic approaches, illustrating that the possible mode was intercalation with a binding number of 2 and a binding constant <i>β</i> of 10<sup>12.87</sup>. Moreover, the label-free electrochemical DNA biosensor exhibited a good linear relationship toward the target gene in a range from 1.00 nM to 50.00 nM with a detection limit as low as 0.046 nM using 3<i>σ</i> estimating system. This facile and low-cost electrochemical method realized the rapid detection and accurate quantification of the target sequence in complicated serum samples, endowing its promising potential in the diagnosis and monitoring of genetic diseases. |
| first_indexed | 2024-03-10T00:35:22Z |
| format | Article |
| id | doaj.art-66525d8dfa02420abefd730fad94896a |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T00:35:22Z |
| publishDate | 2022-09-01 |
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| series | Biosensors |
| spelling | doaj.art-66525d8dfa02420abefd730fad94896a2023-11-23T15:18:29ZengMDPI AGBiosensors2079-63742022-09-0112974710.3390/bios12090747Facile Label-Free Electrochemical DNA Biosensor for Detection of Osteosarcoma-Related Survivin GeneYao Chen0Yu Zhong1Ji-Xing Ye2Yun Lei3Ai-Lin Liu4Department of Pharmaceutical Analysis, Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, The School of Pharmacy, Fujian Medical University, Fuzhou 350122, ChinaDepartment of Pharmaceutical Analysis, Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, The School of Pharmacy, Fujian Medical University, Fuzhou 350122, ChinaDepartment of Pharmaceutical Analysis, Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, The School of Pharmacy, Fujian Medical University, Fuzhou 350122, ChinaDepartment of Pharmaceutical Analysis, Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, The School of Pharmacy, Fujian Medical University, Fuzhou 350122, ChinaDepartment of Pharmaceutical Analysis, Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, The School of Pharmacy, Fujian Medical University, Fuzhou 350122, ChinaA sensitive and selective electrochemical deoxyribonucleic acid (DNA) biosensor was developed for the determination of a osteosarcoma-related survivin gene by using celestine blue (CB) as a label-free hybridization indicator. The proposed strategy adopted a facile and low-cost working electrode with no need for other substances for electrode or DNA functionalization. The interaction mode between CB and DNA was studied by electrochemical and spectroscopic approaches, illustrating that the possible mode was intercalation with a binding number of 2 and a binding constant <i>β</i> of 10<sup>12.87</sup>. Moreover, the label-free electrochemical DNA biosensor exhibited a good linear relationship toward the target gene in a range from 1.00 nM to 50.00 nM with a detection limit as low as 0.046 nM using 3<i>σ</i> estimating system. This facile and low-cost electrochemical method realized the rapid detection and accurate quantification of the target sequence in complicated serum samples, endowing its promising potential in the diagnosis and monitoring of genetic diseases.https://www.mdpi.com/2079-6374/12/9/747electrochemical DNA biosensorcelestine bluehybridization indicatorosteosarcomasurvivin gene |
| spellingShingle | Yao Chen Yu Zhong Ji-Xing Ye Yun Lei Ai-Lin Liu Facile Label-Free Electrochemical DNA Biosensor for Detection of Osteosarcoma-Related Survivin Gene Biosensors electrochemical DNA biosensor celestine blue hybridization indicator osteosarcoma survivin gene |
| title | Facile Label-Free Electrochemical DNA Biosensor for Detection of Osteosarcoma-Related Survivin Gene |
| title_full | Facile Label-Free Electrochemical DNA Biosensor for Detection of Osteosarcoma-Related Survivin Gene |
| title_fullStr | Facile Label-Free Electrochemical DNA Biosensor for Detection of Osteosarcoma-Related Survivin Gene |
| title_full_unstemmed | Facile Label-Free Electrochemical DNA Biosensor for Detection of Osteosarcoma-Related Survivin Gene |
| title_short | Facile Label-Free Electrochemical DNA Biosensor for Detection of Osteosarcoma-Related Survivin Gene |
| title_sort | facile label free electrochemical dna biosensor for detection of osteosarcoma related survivin gene |
| topic | electrochemical DNA biosensor celestine blue hybridization indicator osteosarcoma survivin gene |
| url | https://www.mdpi.com/2079-6374/12/9/747 |
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