Systematic Genome-Wide Study and Expression Analysis of <i>SWEET</i> Gene Family: Sugar Transporter Family Contributes to Biotic and Abiotic Stimuli in Watermelon
The SWEET (Sugars Will Eventually be Exported Transporter) proteins are a novel family of sugar transporters that play key roles in sugar efflux, signal transduction, plant growth and development, plant–pathogen interactions, and stress tolerance. In this study, 22 <i>ClaSWEET</i> genes...
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MDPI AG
2021-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/16/8407 |
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| author | Changqing Xuan Guangpu Lan Fengfei Si Zhilong Zeng Chunxia Wang Vivek Yadav Chunhua Wei Xian Zhang |
| author_facet | Changqing Xuan Guangpu Lan Fengfei Si Zhilong Zeng Chunxia Wang Vivek Yadav Chunhua Wei Xian Zhang |
| author_sort | Changqing Xuan |
| collection | DOAJ |
| description | The SWEET (Sugars Will Eventually be Exported Transporter) proteins are a novel family of sugar transporters that play key roles in sugar efflux, signal transduction, plant growth and development, plant–pathogen interactions, and stress tolerance. In this study, 22 <i>ClaSWEET</i> genes were identified in <i>Citrullus lanatus</i> (Thunb.) through homology searches and classified into four groups by phylogenetic analysis. The genes with similar structures, conserved domains, and motifs were clustered into the same groups. Further analysis of the gene promoter regions uncovered various growth, development, and biotic and abiotic stress responsive <i>cis</i>-regulatory elements. Tissue-specific analysis showed most of the genes were highly expressed in male flowers and the roots of cultivated varieties and wild cultivars. In addition, qRT-PCR results further imply that <i>ClaSWEET</i> proteins might be involved in resistance to <i>Fusarium oxysporum</i> infection. Moreover, a significantly higher expression level of these genes under various abiotic stresses suggests its multifaceted role in mediating plant responses to drought, salt, and low-temperature stress. The genome-wide characterization and phylogenetic analysis of <i>ClaSWEET</i> genes, together with the expression patterns in different tissues and stimuli, lays a solid foundation for future research into their molecular function in watermelon developmental processes and responses to biotic and abiotic stresses. |
| first_indexed | 2024-03-10T08:45:52Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T08:45:52Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-6653ca8556534e228ae55ab8f53c60242023-11-22T07:54:40ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-08-012216840710.3390/ijms22168407Systematic Genome-Wide Study and Expression Analysis of <i>SWEET</i> Gene Family: Sugar Transporter Family Contributes to Biotic and Abiotic Stimuli in WatermelonChangqing Xuan0Guangpu Lan1Fengfei Si2Zhilong Zeng3Chunxia Wang4Vivek Yadav5Chunhua Wei6Xian Zhang7State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A & F University, Yangling 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A & F University, Yangling 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A & F University, Yangling 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A & F University, Yangling 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A & F University, Yangling 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A & F University, Yangling 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A & F University, Yangling 712100, ChinaState Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A & F University, Yangling 712100, ChinaThe SWEET (Sugars Will Eventually be Exported Transporter) proteins are a novel family of sugar transporters that play key roles in sugar efflux, signal transduction, plant growth and development, plant–pathogen interactions, and stress tolerance. In this study, 22 <i>ClaSWEET</i> genes were identified in <i>Citrullus lanatus</i> (Thunb.) through homology searches and classified into four groups by phylogenetic analysis. The genes with similar structures, conserved domains, and motifs were clustered into the same groups. Further analysis of the gene promoter regions uncovered various growth, development, and biotic and abiotic stress responsive <i>cis</i>-regulatory elements. Tissue-specific analysis showed most of the genes were highly expressed in male flowers and the roots of cultivated varieties and wild cultivars. In addition, qRT-PCR results further imply that <i>ClaSWEET</i> proteins might be involved in resistance to <i>Fusarium oxysporum</i> infection. Moreover, a significantly higher expression level of these genes under various abiotic stresses suggests its multifaceted role in mediating plant responses to drought, salt, and low-temperature stress. The genome-wide characterization and phylogenetic analysis of <i>ClaSWEET</i> genes, together with the expression patterns in different tissues and stimuli, lays a solid foundation for future research into their molecular function in watermelon developmental processes and responses to biotic and abiotic stresses.https://www.mdpi.com/1422-0067/22/16/8407<i>Citrullus</i> <i>lanatus</i> (Thunb.)SWEETphylogenetic analysisexpression analysesplant–pathogen interactionabiotic stresses |
| spellingShingle | Changqing Xuan Guangpu Lan Fengfei Si Zhilong Zeng Chunxia Wang Vivek Yadav Chunhua Wei Xian Zhang Systematic Genome-Wide Study and Expression Analysis of <i>SWEET</i> Gene Family: Sugar Transporter Family Contributes to Biotic and Abiotic Stimuli in Watermelon International Journal of Molecular Sciences <i>Citrullus</i> <i>lanatus</i> (Thunb.) SWEET phylogenetic analysis expression analyses plant–pathogen interaction abiotic stresses |
| title | Systematic Genome-Wide Study and Expression Analysis of <i>SWEET</i> Gene Family: Sugar Transporter Family Contributes to Biotic and Abiotic Stimuli in Watermelon |
| title_full | Systematic Genome-Wide Study and Expression Analysis of <i>SWEET</i> Gene Family: Sugar Transporter Family Contributes to Biotic and Abiotic Stimuli in Watermelon |
| title_fullStr | Systematic Genome-Wide Study and Expression Analysis of <i>SWEET</i> Gene Family: Sugar Transporter Family Contributes to Biotic and Abiotic Stimuli in Watermelon |
| title_full_unstemmed | Systematic Genome-Wide Study and Expression Analysis of <i>SWEET</i> Gene Family: Sugar Transporter Family Contributes to Biotic and Abiotic Stimuli in Watermelon |
| title_short | Systematic Genome-Wide Study and Expression Analysis of <i>SWEET</i> Gene Family: Sugar Transporter Family Contributes to Biotic and Abiotic Stimuli in Watermelon |
| title_sort | systematic genome wide study and expression analysis of i sweet i gene family sugar transporter family contributes to biotic and abiotic stimuli in watermelon |
| topic | <i>Citrullus</i> <i>lanatus</i> (Thunb.) SWEET phylogenetic analysis expression analyses plant–pathogen interaction abiotic stresses |
| url | https://www.mdpi.com/1422-0067/22/16/8407 |
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