Determination of Viable Salmonella Typhimurium Cells in Heat Treated Milk By PMA/Real-Time PCR Method
Applying different technological processes during the production of food has a lethal effect on the bacteria but DNA of these bacterial strains may cause false positive results when detected by real time PCR technique because they preserve their existence for a certain period of time. To overcome th...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Turkish Science and Technology Publishing (TURSTEP)
2017-06-01
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| Series: | Turkish Journal of Agriculture: Food Science and Technology |
| Subjects: | |
| Online Access: | http://www.agrifoodscience.com/index.php/TURJAF/article/view/1094 |
| _version_ | 1828015205903237120 |
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| author | Zülal Kesmen Hakiye Aslan |
| author_facet | Zülal Kesmen Hakiye Aslan |
| author_sort | Zülal Kesmen |
| collection | DOAJ |
| description | Applying different technological processes during the production of food has a lethal effect on the bacteria but DNA of these bacterial strains may cause false positive results when detected by real time PCR technique because they preserve their existence for a certain period of time. To overcome this shortcoming of the real time PCR technique, a new method has been developed in recent years, based on the removal of dead cell DNA from the medium by treatment with Propodium Monoazide (PMA) before DNA extraction. In this study, real-time PCR method was combined with PMA application for the detection of live cells of Salmonella Typhimurium in heat treated milk samples. For this purpose, milk samples inoculated with S. Tyhimurium were heat treated at different temperatures (60, 65, 70 and 75°C) and times (15, 60, 300, 900 sec) and number of live bacteria was determined comparatively by direct real-time PCR, PMA/real-time PCR and conventional cultural method. As a result, unlike the direct real time PCR technique, PMA/real-time PCR method prevents to a certain extent of false positive results from dead cells at all tested temperatures and times but higher results were obtained from PMA/real-time PCR method when compared to conventional cultural results. Therefore, further studies should be carried out to optimize the conditions of the PMA application in order to eliminate the high positive results detected by the PMA / real-time PCR method |
| first_indexed | 2024-04-10T10:13:27Z |
| format | Article |
| id | doaj.art-66548aaf30704c9fb5ddf98cab5c9a4c |
| institution | Directory Open Access Journal |
| issn | 2148-127X |
| language | English |
| last_indexed | 2024-04-10T10:13:27Z |
| publishDate | 2017-06-01 |
| publisher | Turkish Science and Technology Publishing (TURSTEP) |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj.art-66548aaf30704c9fb5ddf98cab5c9a4c2023-02-15T16:21:59ZengTurkish Science and Technology Publishing (TURSTEP)Turkish Journal of Agriculture: Food Science and Technology2148-127X2017-06-015551852410.24925/turjaf.v5i5.518-524.1094532Determination of Viable Salmonella Typhimurium Cells in Heat Treated Milk By PMA/Real-Time PCR MethodZülal Kesmen0Hakiye Aslan1Erciyes Üniversitesi, Mühendislik Fakültesi, Gıda Mühendisliği Bölümü, 38039 Kayseri,Bingöl Üniversitesi, Mühendislik-Mimarlık Fakültesi, Gıda Mühendisliği Bölümü, 12000 Bingöl,Applying different technological processes during the production of food has a lethal effect on the bacteria but DNA of these bacterial strains may cause false positive results when detected by real time PCR technique because they preserve their existence for a certain period of time. To overcome this shortcoming of the real time PCR technique, a new method has been developed in recent years, based on the removal of dead cell DNA from the medium by treatment with Propodium Monoazide (PMA) before DNA extraction. In this study, real-time PCR method was combined with PMA application for the detection of live cells of Salmonella Typhimurium in heat treated milk samples. For this purpose, milk samples inoculated with S. Tyhimurium were heat treated at different temperatures (60, 65, 70 and 75°C) and times (15, 60, 300, 900 sec) and number of live bacteria was determined comparatively by direct real-time PCR, PMA/real-time PCR and conventional cultural method. As a result, unlike the direct real time PCR technique, PMA/real-time PCR method prevents to a certain extent of false positive results from dead cells at all tested temperatures and times but higher results were obtained from PMA/real-time PCR method when compared to conventional cultural results. Therefore, further studies should be carried out to optimize the conditions of the PMA application in order to eliminate the high positive results detected by the PMA / real-time PCR methodhttp://www.agrifoodscience.com/index.php/TURJAF/article/view/1094Real-time PCRPropidium monoazideCanlı-ölü bakteri ayrımıSalmonella TyphimuriumSüt |
| spellingShingle | Zülal Kesmen Hakiye Aslan Determination of Viable Salmonella Typhimurium Cells in Heat Treated Milk By PMA/Real-Time PCR Method Turkish Journal of Agriculture: Food Science and Technology Real-time PCR Propidium monoazide Canlı-ölü bakteri ayrımı Salmonella Typhimurium Süt |
| title | Determination of Viable Salmonella Typhimurium Cells in Heat Treated Milk By PMA/Real-Time PCR Method |
| title_full | Determination of Viable Salmonella Typhimurium Cells in Heat Treated Milk By PMA/Real-Time PCR Method |
| title_fullStr | Determination of Viable Salmonella Typhimurium Cells in Heat Treated Milk By PMA/Real-Time PCR Method |
| title_full_unstemmed | Determination of Viable Salmonella Typhimurium Cells in Heat Treated Milk By PMA/Real-Time PCR Method |
| title_short | Determination of Viable Salmonella Typhimurium Cells in Heat Treated Milk By PMA/Real-Time PCR Method |
| title_sort | determination of viable salmonella typhimurium cells in heat treated milk by pma real time pcr method |
| topic | Real-time PCR Propidium monoazide Canlı-ölü bakteri ayrımı Salmonella Typhimurium Süt |
| url | http://www.agrifoodscience.com/index.php/TURJAF/article/view/1094 |
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