Localization of Neurotrophin Specific Trk Receptors in Mechanosensory Systems of Killifish (<i>Nothobranchius guentheri)</i>
Neurotrophins (NTs) and their signal-transducing Trk receptors play a crucial role in the development and maintenance of specific neuronal subpopulations in nervous and sensory systems. NTs are supposed to regulate two sensory systems in fish, the inner ear and the lateral line system (LLS). The lat...
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| Format: | Article |
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MDPI AG
2021-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/19/10411 |
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| author | Marialuisa Aragona Caterina Porcino Maria Cristina Guerrera Giuseppe Montalbano Maria Levanti Francesco Abbate Rosaria Laurà Antonino Germanà |
| author_facet | Marialuisa Aragona Caterina Porcino Maria Cristina Guerrera Giuseppe Montalbano Maria Levanti Francesco Abbate Rosaria Laurà Antonino Germanà |
| author_sort | Marialuisa Aragona |
| collection | DOAJ |
| description | Neurotrophins (NTs) and their signal-transducing Trk receptors play a crucial role in the development and maintenance of specific neuronal subpopulations in nervous and sensory systems. NTs are supposed to regulate two sensory systems in fish, the inner ear and the lateral line system (LLS). The latter is one of the major mechanosensory systems in fish. Considering that annual fishes of the genus <i>Nothobranchius</i>, with their short life expectancy, have become a suitable model for aging studies and that the occurrence and distribution of neurotrophin Trk receptors have never been investigated in the inner ear and LLS of killifish (<i>Nothobranchius guentheri</i>), our study aimed to investigate the localization of neurotrophin-specific Trk receptors in mechanosensory systems of <i>N. guentheri</i>. For histological and immunohistochemical analysis, adult specimens of <i>N. guentheri</i> were processed using antibodies against Trk receptors and S100 protein. An intense immunoreaction for TrkA and TrkC was found in the sensory cells of the inner ear as well as in the hair cells of LLS. Moreover, also the neurons localized in the acoustic ganglia displayed a specific immunoreaction for all Trk receptors (TrkA, B, and C) analyzed. Taken together, our results demonstrate, for the first time, that neurotrophins and their specific receptors could play a pivotal role in the biology of the sensory cells of the inner ear and LLS of <i>N. guentheri</i> and might also be involved in the hair cells regeneration process in normal and aged conditions. |
| first_indexed | 2024-03-10T06:59:51Z |
| format | Article |
| id | doaj.art-665490d90dc141cdb4f077f79c30e33a |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T06:59:51Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-665490d90dc141cdb4f077f79c30e33a2023-11-22T16:09:10ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-09-0122191041110.3390/ijms221910411Localization of Neurotrophin Specific Trk Receptors in Mechanosensory Systems of Killifish (<i>Nothobranchius guentheri)</i>Marialuisa Aragona0Caterina Porcino1Maria Cristina Guerrera2Giuseppe Montalbano3Maria Levanti4Francesco Abbate5Rosaria Laurà6Antonino Germanà7Zebrafish Neuromorphology Laboratory, Department of Veterinary Sciences, University of Messina, 98168 Messina, ItalyZebrafish Neuromorphology Laboratory, Department of Veterinary Sciences, University of Messina, 98168 Messina, ItalyZebrafish Neuromorphology Laboratory, Department of Veterinary Sciences, University of Messina, 98168 Messina, ItalyZebrafish Neuromorphology Laboratory, Department of Veterinary Sciences, University of Messina, 98168 Messina, ItalyZebrafish Neuromorphology Laboratory, Department of Veterinary Sciences, University of Messina, 98168 Messina, ItalyZebrafish Neuromorphology Laboratory, Department of Veterinary Sciences, University of Messina, 98168 Messina, ItalyZebrafish Neuromorphology Laboratory, Department of Veterinary Sciences, University of Messina, 98168 Messina, ItalyZebrafish Neuromorphology Laboratory, Department of Veterinary Sciences, University of Messina, 98168 Messina, ItalyNeurotrophins (NTs) and their signal-transducing Trk receptors play a crucial role in the development and maintenance of specific neuronal subpopulations in nervous and sensory systems. NTs are supposed to regulate two sensory systems in fish, the inner ear and the lateral line system (LLS). The latter is one of the major mechanosensory systems in fish. Considering that annual fishes of the genus <i>Nothobranchius</i>, with their short life expectancy, have become a suitable model for aging studies and that the occurrence and distribution of neurotrophin Trk receptors have never been investigated in the inner ear and LLS of killifish (<i>Nothobranchius guentheri</i>), our study aimed to investigate the localization of neurotrophin-specific Trk receptors in mechanosensory systems of <i>N. guentheri</i>. For histological and immunohistochemical analysis, adult specimens of <i>N. guentheri</i> were processed using antibodies against Trk receptors and S100 protein. An intense immunoreaction for TrkA and TrkC was found in the sensory cells of the inner ear as well as in the hair cells of LLS. Moreover, also the neurons localized in the acoustic ganglia displayed a specific immunoreaction for all Trk receptors (TrkA, B, and C) analyzed. Taken together, our results demonstrate, for the first time, that neurotrophins and their specific receptors could play a pivotal role in the biology of the sensory cells of the inner ear and LLS of <i>N. guentheri</i> and might also be involved in the hair cells regeneration process in normal and aged conditions.https://www.mdpi.com/1422-0067/22/19/10411killifish<i>Nothobranchius guentheri</i>inner earlateral line systemfree neuromastneurotrophin |
| spellingShingle | Marialuisa Aragona Caterina Porcino Maria Cristina Guerrera Giuseppe Montalbano Maria Levanti Francesco Abbate Rosaria Laurà Antonino Germanà Localization of Neurotrophin Specific Trk Receptors in Mechanosensory Systems of Killifish (<i>Nothobranchius guentheri)</i> International Journal of Molecular Sciences killifish <i>Nothobranchius guentheri</i> inner ear lateral line system free neuromast neurotrophin |
| title | Localization of Neurotrophin Specific Trk Receptors in Mechanosensory Systems of Killifish (<i>Nothobranchius guentheri)</i> |
| title_full | Localization of Neurotrophin Specific Trk Receptors in Mechanosensory Systems of Killifish (<i>Nothobranchius guentheri)</i> |
| title_fullStr | Localization of Neurotrophin Specific Trk Receptors in Mechanosensory Systems of Killifish (<i>Nothobranchius guentheri)</i> |
| title_full_unstemmed | Localization of Neurotrophin Specific Trk Receptors in Mechanosensory Systems of Killifish (<i>Nothobranchius guentheri)</i> |
| title_short | Localization of Neurotrophin Specific Trk Receptors in Mechanosensory Systems of Killifish (<i>Nothobranchius guentheri)</i> |
| title_sort | localization of neurotrophin specific trk receptors in mechanosensory systems of killifish i nothobranchius guentheri i |
| topic | killifish <i>Nothobranchius guentheri</i> inner ear lateral line system free neuromast neurotrophin |
| url | https://www.mdpi.com/1422-0067/22/19/10411 |
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