Identification of multiple novel genetic mechanisms that regulate chilling tolerance in Arabidopsis
IntroductionCold stress adversely affects the growth and development of plants and limits the geographical distribution of many plant species. Accumulation of spontaneous mutations shapes the adaptation of plant species to diverse climatic conditions.MethodsThe genome-wide association study of the p...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1094462/full |
| _version_ | 1797955206255738880 |
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| author | Dipak Kumar Sahoo Chinmay Hegde Madan K. Bhattacharyya |
| author_facet | Dipak Kumar Sahoo Chinmay Hegde Madan K. Bhattacharyya |
| author_sort | Dipak Kumar Sahoo |
| collection | DOAJ |
| description | IntroductionCold stress adversely affects the growth and development of plants and limits the geographical distribution of many plant species. Accumulation of spontaneous mutations shapes the adaptation of plant species to diverse climatic conditions.MethodsThe genome-wide association study of the phenotypic variation gathered by a newly designed phenomic platform with the over six millions single nucleotide polymorphic (SNP) loci distributed across the genomes of 417 Arabidopsis natural variants collected from various geographical regions revealed 33 candidate cold responsive genes.ResultsInvestigation of at least two independent insertion mutants for 29 genes identified 16 chilling tolerance genes governing diverse genetic mechanisms. Five of these genes encode novel leucine-rich repeat domain-containing proteins including three nucleotide-binding site-leucine-rich repeat (NBS-LRR) proteins. Among the 16 identified chilling tolerance genes, ADS2 and ACD6 are the only two chilling tolerance genes identified earlier.DiscussionThe 12.5% overlap between the genes identified in this genome-wide association study (GWAS) of natural variants with those discovered previously through forward and reverse genetic approaches suggests that chilling tolerance is a complex physiological process governed by a large number of genetic mechanisms. |
| first_indexed | 2024-04-10T23:29:48Z |
| format | Article |
| id | doaj.art-6655f4308ad74252b6bdcd50f14a2d53 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-10T23:29:48Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-6655f4308ad74252b6bdcd50f14a2d532023-01-12T07:02:30ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-01-011310.3389/fpls.2022.10944621094462Identification of multiple novel genetic mechanisms that regulate chilling tolerance in ArabidopsisDipak Kumar Sahoo0Chinmay Hegde1Madan K. Bhattacharyya2Department of Agronomy, Iowa State University, Ames, IA, United StatesDepartment of Electrical and Computer Engineering, Iowa State University, Ames, IA, United StatesDepartment of Agronomy, Iowa State University, Ames, IA, United StatesIntroductionCold stress adversely affects the growth and development of plants and limits the geographical distribution of many plant species. Accumulation of spontaneous mutations shapes the adaptation of plant species to diverse climatic conditions.MethodsThe genome-wide association study of the phenotypic variation gathered by a newly designed phenomic platform with the over six millions single nucleotide polymorphic (SNP) loci distributed across the genomes of 417 Arabidopsis natural variants collected from various geographical regions revealed 33 candidate cold responsive genes.ResultsInvestigation of at least two independent insertion mutants for 29 genes identified 16 chilling tolerance genes governing diverse genetic mechanisms. Five of these genes encode novel leucine-rich repeat domain-containing proteins including three nucleotide-binding site-leucine-rich repeat (NBS-LRR) proteins. Among the 16 identified chilling tolerance genes, ADS2 and ACD6 are the only two chilling tolerance genes identified earlier.DiscussionThe 12.5% overlap between the genes identified in this genome-wide association study (GWAS) of natural variants with those discovered previously through forward and reverse genetic approaches suggests that chilling tolerance is a complex physiological process governed by a large number of genetic mechanisms.https://www.frontiersin.org/articles/10.3389/fpls.2022.1094462/fullcold tolerance mechanismsArabidopsisphenomics platformmutatnt analysesGWASNBS-LRR |
| spellingShingle | Dipak Kumar Sahoo Chinmay Hegde Madan K. Bhattacharyya Identification of multiple novel genetic mechanisms that regulate chilling tolerance in Arabidopsis Frontiers in Plant Science cold tolerance mechanisms Arabidopsis phenomics platform mutatnt analyses GWAS NBS-LRR |
| title | Identification of multiple novel genetic mechanisms that regulate chilling tolerance in Arabidopsis |
| title_full | Identification of multiple novel genetic mechanisms that regulate chilling tolerance in Arabidopsis |
| title_fullStr | Identification of multiple novel genetic mechanisms that regulate chilling tolerance in Arabidopsis |
| title_full_unstemmed | Identification of multiple novel genetic mechanisms that regulate chilling tolerance in Arabidopsis |
| title_short | Identification of multiple novel genetic mechanisms that regulate chilling tolerance in Arabidopsis |
| title_sort | identification of multiple novel genetic mechanisms that regulate chilling tolerance in arabidopsis |
| topic | cold tolerance mechanisms Arabidopsis phenomics platform mutatnt analyses GWAS NBS-LRR |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1094462/full |
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