Mitochondrial Consequences of Organ Preservation Techniques during Liver Transplantation
Allograft ischemia during liver transplantation (LT) adversely affects the function of mitochondria, resulting in impairment of oxidative phosphorylation and compromised post-transplant recovery of the affected organ. Several preservation methods have been developed to improve donor organ quality; h...
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MDPI AG
2021-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/6/2816 |
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| author | Tamara Horváth Dávid Kurszán Jász Bálint Baráth Marietta Zita Poles Mihály Boros Petra Hartmann |
| author_facet | Tamara Horváth Dávid Kurszán Jász Bálint Baráth Marietta Zita Poles Mihály Boros Petra Hartmann |
| author_sort | Tamara Horváth |
| collection | DOAJ |
| description | Allograft ischemia during liver transplantation (LT) adversely affects the function of mitochondria, resulting in impairment of oxidative phosphorylation and compromised post-transplant recovery of the affected organ. Several preservation methods have been developed to improve donor organ quality; however, their effects on mitochondrial functions have not yet been compared. This study aimed to summarize the available data on mitochondrial effects of graft preservation methods in preclinical models of LT. Furthermore, a network meta-analysis was conducted to determine if any of these treatments provide a superior benefit, suggesting that they might be used on humans. A systematic search was conducted using electronic databases (EMBASE, MEDLINE (via PubMed), the Cochrane Central Register of Controlled Trials (CENTRAL) and Web of Science) for controlled animal studies using preservation methods for LT. The ATP content of the graft was the primary outcome, as this is an indicator overall mitochondrial function. Secondary outcomes were the respiratory activity of mitochondrial complexes, cytochrome c and aspartate aminotransferase (ALT) release. Both a random-effects model and the SYRCLE risk of bias analysis for animal studies were used. After a comprehensive search of the databases, 25 studies were enrolled in the analysis. Treatments that had the most significant protective effect on ATP content included hypothermic and subnormothermic machine perfusion (HMP and SNMP) (MD = −1.0, 95% CI: (−2.3, 0.3) and MD = −1.1, 95% CI: (−3.2, 1.02)), while the effects of warm ischemia (WI) without cold storage (WI) and normothermic machine perfusion (NMP) were less pronounced (MD = −1.8, 95% CI: (−2.9, −0.7) and MD = −2.1 MD; CI: (−4.6; 0.4)). The subgroup of static cold storage (SCS) with shorter preservation time (< 12 h) yielded better results than SCS ≥ 12 h, NMP and WI, in terms of ATP preservation and the respiratory capacity of complexes. HMP and SNMP stand out in terms of mitochondrial protection when compared to other treatments for LT in animals. The shorter storage time at lower temperatures, together with the dynamic preservation, provided superior protection for the grafts in terms of mitochondrial function. Additional clinical studies on human patients including marginal donors and longer ischemia times are needed to confirm any superiority of preservation methods with respect to mitochondrial function. |
| first_indexed | 2024-03-10T13:21:16Z |
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| issn | 1661-6596 1422-0067 |
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| spelling | doaj.art-66562415f4b64e77bb2502dbd7895c252023-11-21T09:58:07ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-03-01226281610.3390/ijms22062816Mitochondrial Consequences of Organ Preservation Techniques during Liver TransplantationTamara Horváth0Dávid Kurszán Jász1Bálint Baráth2Marietta Zita Poles3Mihály Boros4Petra Hartmann5Institute of Surgical Research, University of Szeged, 6724 Szeged, HungaryInstitute of Surgical Research, University of Szeged, 6724 Szeged, HungaryInstitute of Surgical Research, University of Szeged, 6724 Szeged, HungaryInstitute of Surgical Research, University of Szeged, 6724 Szeged, HungaryInstitute of Surgical Research, University of Szeged, 6724 Szeged, HungaryInstitute of Surgical Research, University of Szeged, 6724 Szeged, HungaryAllograft ischemia during liver transplantation (LT) adversely affects the function of mitochondria, resulting in impairment of oxidative phosphorylation and compromised post-transplant recovery of the affected organ. Several preservation methods have been developed to improve donor organ quality; however, their effects on mitochondrial functions have not yet been compared. This study aimed to summarize the available data on mitochondrial effects of graft preservation methods in preclinical models of LT. Furthermore, a network meta-analysis was conducted to determine if any of these treatments provide a superior benefit, suggesting that they might be used on humans. A systematic search was conducted using electronic databases (EMBASE, MEDLINE (via PubMed), the Cochrane Central Register of Controlled Trials (CENTRAL) and Web of Science) for controlled animal studies using preservation methods for LT. The ATP content of the graft was the primary outcome, as this is an indicator overall mitochondrial function. Secondary outcomes were the respiratory activity of mitochondrial complexes, cytochrome c and aspartate aminotransferase (ALT) release. Both a random-effects model and the SYRCLE risk of bias analysis for animal studies were used. After a comprehensive search of the databases, 25 studies were enrolled in the analysis. Treatments that had the most significant protective effect on ATP content included hypothermic and subnormothermic machine perfusion (HMP and SNMP) (MD = −1.0, 95% CI: (−2.3, 0.3) and MD = −1.1, 95% CI: (−3.2, 1.02)), while the effects of warm ischemia (WI) without cold storage (WI) and normothermic machine perfusion (NMP) were less pronounced (MD = −1.8, 95% CI: (−2.9, −0.7) and MD = −2.1 MD; CI: (−4.6; 0.4)). The subgroup of static cold storage (SCS) with shorter preservation time (< 12 h) yielded better results than SCS ≥ 12 h, NMP and WI, in terms of ATP preservation and the respiratory capacity of complexes. HMP and SNMP stand out in terms of mitochondrial protection when compared to other treatments for LT in animals. The shorter storage time at lower temperatures, together with the dynamic preservation, provided superior protection for the grafts in terms of mitochondrial function. Additional clinical studies on human patients including marginal donors and longer ischemia times are needed to confirm any superiority of preservation methods with respect to mitochondrial function.https://www.mdpi.com/1422-0067/22/6/2816liver transplantationgraft preservationmitochondrial functionsanimal studiesmeta-analysis |
| spellingShingle | Tamara Horváth Dávid Kurszán Jász Bálint Baráth Marietta Zita Poles Mihály Boros Petra Hartmann Mitochondrial Consequences of Organ Preservation Techniques during Liver Transplantation International Journal of Molecular Sciences liver transplantation graft preservation mitochondrial functions animal studies meta-analysis |
| title | Mitochondrial Consequences of Organ Preservation Techniques during Liver Transplantation |
| title_full | Mitochondrial Consequences of Organ Preservation Techniques during Liver Transplantation |
| title_fullStr | Mitochondrial Consequences of Organ Preservation Techniques during Liver Transplantation |
| title_full_unstemmed | Mitochondrial Consequences of Organ Preservation Techniques during Liver Transplantation |
| title_short | Mitochondrial Consequences of Organ Preservation Techniques during Liver Transplantation |
| title_sort | mitochondrial consequences of organ preservation techniques during liver transplantation |
| topic | liver transplantation graft preservation mitochondrial functions animal studies meta-analysis |
| url | https://www.mdpi.com/1422-0067/22/6/2816 |
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