Using stable isotope tracing to unravel the metabolic components of neurodegeneration: Focus on neuron-glia metabolic interactions
Disrupted brain metabolism is a critical component of several neurodegenerative diseases. Energy metabolism of both neurons and astrocytes is closely connected to neurotransmitter recycling via the glutamate/GABA-glutamine cycle. Neurons and astrocytes hereby work in close metabolic collaboration wh...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Neurobiology of Disease |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0969996123001596 |
| _version_ | 1797811061258190848 |
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| author | Emil W. Westi Jens V. Andersen Blanca I. Aldana |
| author_facet | Emil W. Westi Jens V. Andersen Blanca I. Aldana |
| author_sort | Emil W. Westi |
| collection | DOAJ |
| description | Disrupted brain metabolism is a critical component of several neurodegenerative diseases. Energy metabolism of both neurons and astrocytes is closely connected to neurotransmitter recycling via the glutamate/GABA-glutamine cycle. Neurons and astrocytes hereby work in close metabolic collaboration which is essential to sustain neurotransmission. Elucidating the mechanistic involvement of altered brain metabolism in disease progression has been aided by the advance of techniques to monitor cellular metabolism, in particular by mapping metabolism of substrates containing stable isotopes, a technique known as isotope tracing. Here we review key aspects of isotope tracing including advantages, drawbacks and applications to different cerebral preparations. In addition, we narrate how isotope tracing has facilitated the discovery of central metabolic features in neurodegeneration with a focus on the metabolic cooperation between neurons and astrocytes. |
| first_indexed | 2024-03-13T07:18:03Z |
| format | Article |
| id | doaj.art-6656a266cf8b4054b5dc79a3b802c18a |
| institution | Directory Open Access Journal |
| issn | 1095-953X |
| language | English |
| last_indexed | 2024-03-13T07:18:03Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neurobiology of Disease |
| spelling | doaj.art-6656a266cf8b4054b5dc79a3b802c18a2023-06-05T04:12:32ZengElsevierNeurobiology of Disease1095-953X2023-06-01182106145Using stable isotope tracing to unravel the metabolic components of neurodegeneration: Focus on neuron-glia metabolic interactionsEmil W. Westi0Jens V. Andersen1Blanca I. Aldana2Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, DenmarkDepartment of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, DenmarkCorresponding author at: Department of Drug Design and Pharmacology, Universitetsparken 2, 2100 Copenhagen E, Denmark.; Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, DenmarkDisrupted brain metabolism is a critical component of several neurodegenerative diseases. Energy metabolism of both neurons and astrocytes is closely connected to neurotransmitter recycling via the glutamate/GABA-glutamine cycle. Neurons and astrocytes hereby work in close metabolic collaboration which is essential to sustain neurotransmission. Elucidating the mechanistic involvement of altered brain metabolism in disease progression has been aided by the advance of techniques to monitor cellular metabolism, in particular by mapping metabolism of substrates containing stable isotopes, a technique known as isotope tracing. Here we review key aspects of isotope tracing including advantages, drawbacks and applications to different cerebral preparations. In addition, we narrate how isotope tracing has facilitated the discovery of central metabolic features in neurodegeneration with a focus on the metabolic cooperation between neurons and astrocytes.http://www.sciencedirect.com/science/article/pii/S0969996123001596Brain energy metabolismAstrocytesMass spectrometryNeurodegenerative disordersAlzheimer's diseaseParkinson's disease |
| spellingShingle | Emil W. Westi Jens V. Andersen Blanca I. Aldana Using stable isotope tracing to unravel the metabolic components of neurodegeneration: Focus on neuron-glia metabolic interactions Neurobiology of Disease Brain energy metabolism Astrocytes Mass spectrometry Neurodegenerative disorders Alzheimer's disease Parkinson's disease |
| title | Using stable isotope tracing to unravel the metabolic components of neurodegeneration: Focus on neuron-glia metabolic interactions |
| title_full | Using stable isotope tracing to unravel the metabolic components of neurodegeneration: Focus on neuron-glia metabolic interactions |
| title_fullStr | Using stable isotope tracing to unravel the metabolic components of neurodegeneration: Focus on neuron-glia metabolic interactions |
| title_full_unstemmed | Using stable isotope tracing to unravel the metabolic components of neurodegeneration: Focus on neuron-glia metabolic interactions |
| title_short | Using stable isotope tracing to unravel the metabolic components of neurodegeneration: Focus on neuron-glia metabolic interactions |
| title_sort | using stable isotope tracing to unravel the metabolic components of neurodegeneration focus on neuron glia metabolic interactions |
| topic | Brain energy metabolism Astrocytes Mass spectrometry Neurodegenerative disorders Alzheimer's disease Parkinson's disease |
| url | http://www.sciencedirect.com/science/article/pii/S0969996123001596 |
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