Conditioned Media from Human Pulp Stem Cell Cultures Improve Bone Regeneration in Rat Calvarial Critical-Size Defects
The aim of this study was to test whether lyophilized conditioned media from human dental pulp mesenchymal stem cell cultures promote the healing of critical-size defects created in the calvaria of rats. Prior to the surgical procedure, the medium in which dental pulp stem cells were cultured was fr...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/14/8/396 |
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| author | Leonardo Fernandes Buss Gustavo Sigrist de Martin Elizabeth Ferreira Martinez Isabela Amanda de Abreu Araújo Porcaro Filgueiras José Luiz Magnabosco Bruno Frenhan Alves Bruno de Macedo Almeida Tatiana Kotaka Marcelo Lucchesi Teixeira José Ricardo Muniz Ferreira Daniel Navarro da Rocha Raul Canal Antonio Carlos Aloise Lexie Shannon Holliday André Antonio Pelegrine |
| author_facet | Leonardo Fernandes Buss Gustavo Sigrist de Martin Elizabeth Ferreira Martinez Isabela Amanda de Abreu Araújo Porcaro Filgueiras José Luiz Magnabosco Bruno Frenhan Alves Bruno de Macedo Almeida Tatiana Kotaka Marcelo Lucchesi Teixeira José Ricardo Muniz Ferreira Daniel Navarro da Rocha Raul Canal Antonio Carlos Aloise Lexie Shannon Holliday André Antonio Pelegrine |
| author_sort | Leonardo Fernandes Buss |
| collection | DOAJ |
| description | The aim of this study was to test whether lyophilized conditioned media from human dental pulp mesenchymal stem cell cultures promote the healing of critical-size defects created in the calvaria of rats. Prior to the surgical procedure, the medium in which dental pulp stem cells were cultured was frozen and lyophilized. After general anesthesia, an 8 mm diameter bone defect was created in the calvaria of twenty-four rats. The defects were filled with the following materials: xenograft alone (G1) or xenograft associated with lyophilized conditioned medium (G2). After 14 or 42 days, the animals were euthanized, and the specimens processed for histologic and immunohistochemical analysis. Bone formation at the center of the defect was observed only in the G2 at 42 days. At both timepoints, increased staining for VEGF, a marker for angiogenesis, was observed in G2. Consistent with this, at 14 days, G2 also had a higher number of blood vessels detected by immunostaining with an anti-CD34 antibody. In conclusion, conditioned media from human dental pulp mesenchymal stem cell cultures had a positive effect on the regenerative process in rat critical-size bone defects. Both the formation of bone and enhancement of vascularization were stimulated by the conditioned media. |
| first_indexed | 2024-03-10T23:50:26Z |
| format | Article |
| id | doaj.art-665827f45cc542fe9b9f47d25cf418ea |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-10T23:50:26Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-665827f45cc542fe9b9f47d25cf418ea2023-11-19T01:42:53ZengMDPI AGJournal of Functional Biomaterials2079-49832023-07-0114839610.3390/jfb14080396Conditioned Media from Human Pulp Stem Cell Cultures Improve Bone Regeneration in Rat Calvarial Critical-Size DefectsLeonardo Fernandes Buss0Gustavo Sigrist de Martin1Elizabeth Ferreira Martinez2Isabela Amanda de Abreu Araújo Porcaro Filgueiras3José Luiz Magnabosco4Bruno Frenhan Alves5Bruno de Macedo Almeida6Tatiana Kotaka7Marcelo Lucchesi Teixeira8José Ricardo Muniz Ferreira9Daniel Navarro da Rocha10Raul Canal11Antonio Carlos Aloise12Lexie Shannon Holliday13André Antonio Pelegrine14Faculdade de Odontologia São Leopoldo Mandic, Campinas 13045-755, SP, BrazilFaculdade de Odontologia São Leopoldo Mandic, Campinas 13045-755, SP, BrazilDivision of Cell Biology, Faculdade São Leopoldo Mandic, Campinas 13045-755, SP, BrazilFaculdade de Odontologia São Leopoldo Mandic, Campinas 13045-755, SP, BrazilFaculdade de Odontologia São Leopoldo Mandic, Campinas 13045-755, SP, BrazilFaculdade de Odontologia São Leopoldo Mandic, Campinas 13045-755, SP, BrazilFaculdade de Odontologia São Leopoldo Mandic, Campinas 13045-755, SP, BrazilFaculdade de Odontologia São Leopoldo Mandic, Campinas 13045-755, SP, BrazilDivision of Prosthodontics, Faculdade São Leopoldo Mandic, Campinas 13045-755, SP, BrazilRCrio Bioengenharia, Campinas 13098-324, SP, BrazilRCrio Bioengenharia, Campinas 13098-324, SP, BrazilANADEM, Brasília 70322-901, DF, BrazilDivision of Oral Implantology, Faculdade São Leopoldo Mandic, Campinas 13045-755, SP, BrazilDepartment of Orthodontics, University of Florida, Gainesville, FL 32611, USADivision of Oral Implantology, Faculdade São Leopoldo Mandic, Campinas 13045-755, SP, BrazilThe aim of this study was to test whether lyophilized conditioned media from human dental pulp mesenchymal stem cell cultures promote the healing of critical-size defects created in the calvaria of rats. Prior to the surgical procedure, the medium in which dental pulp stem cells were cultured was frozen and lyophilized. After general anesthesia, an 8 mm diameter bone defect was created in the calvaria of twenty-four rats. The defects were filled with the following materials: xenograft alone (G1) or xenograft associated with lyophilized conditioned medium (G2). After 14 or 42 days, the animals were euthanized, and the specimens processed for histologic and immunohistochemical analysis. Bone formation at the center of the defect was observed only in the G2 at 42 days. At both timepoints, increased staining for VEGF, a marker for angiogenesis, was observed in G2. Consistent with this, at 14 days, G2 also had a higher number of blood vessels detected by immunostaining with an anti-CD34 antibody. In conclusion, conditioned media from human dental pulp mesenchymal stem cell cultures had a positive effect on the regenerative process in rat critical-size bone defects. Both the formation of bone and enhancement of vascularization were stimulated by the conditioned media.https://www.mdpi.com/2079-4983/14/8/396bone regenerationstem cellsosteogenesisconditioned media |
| spellingShingle | Leonardo Fernandes Buss Gustavo Sigrist de Martin Elizabeth Ferreira Martinez Isabela Amanda de Abreu Araújo Porcaro Filgueiras José Luiz Magnabosco Bruno Frenhan Alves Bruno de Macedo Almeida Tatiana Kotaka Marcelo Lucchesi Teixeira José Ricardo Muniz Ferreira Daniel Navarro da Rocha Raul Canal Antonio Carlos Aloise Lexie Shannon Holliday André Antonio Pelegrine Conditioned Media from Human Pulp Stem Cell Cultures Improve Bone Regeneration in Rat Calvarial Critical-Size Defects Journal of Functional Biomaterials bone regeneration stem cells osteogenesis conditioned media |
| title | Conditioned Media from Human Pulp Stem Cell Cultures Improve Bone Regeneration in Rat Calvarial Critical-Size Defects |
| title_full | Conditioned Media from Human Pulp Stem Cell Cultures Improve Bone Regeneration in Rat Calvarial Critical-Size Defects |
| title_fullStr | Conditioned Media from Human Pulp Stem Cell Cultures Improve Bone Regeneration in Rat Calvarial Critical-Size Defects |
| title_full_unstemmed | Conditioned Media from Human Pulp Stem Cell Cultures Improve Bone Regeneration in Rat Calvarial Critical-Size Defects |
| title_short | Conditioned Media from Human Pulp Stem Cell Cultures Improve Bone Regeneration in Rat Calvarial Critical-Size Defects |
| title_sort | conditioned media from human pulp stem cell cultures improve bone regeneration in rat calvarial critical size defects |
| topic | bone regeneration stem cells osteogenesis conditioned media |
| url | https://www.mdpi.com/2079-4983/14/8/396 |
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