A Step-by-Step Approach to Improve Clinical Translation of Liposome-Based Nanomaterials, a Focus on Innate Immune and Inflammatory Responses
This study aims to provide guidelines to design and perform a robust and reliable physical-chemical characterization of liposome-based nanomaterials, and to support method development with a specific focus on their inflammation-inducing potential. Out of eight differently functionalized liposomes se...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/2/820 |
| _version_ | 1797411155572948992 |
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| author | Giacomo Della Camera Dorelia Lipsa Dora Mehn Paola Italiani Diana Boraschi Sabrina Gioria |
| author_facet | Giacomo Della Camera Dorelia Lipsa Dora Mehn Paola Italiani Diana Boraschi Sabrina Gioria |
| author_sort | Giacomo Della Camera |
| collection | DOAJ |
| description | This study aims to provide guidelines to design and perform a robust and reliable physical-chemical characterization of liposome-based nanomaterials, and to support method development with a specific focus on their inflammation-inducing potential. Out of eight differently functionalized liposomes selected as “case-studies”, three passed the physical-chemical characterization (in terms of size-distribution, homogeneity and stability) and the screening for bacterial contamination (sterility and apyrogenicity). Although all three were non-cytotoxic when tested in vitro, they showed a different capacity to activate human blood cells. HSPC/CHOL-coated liposomes elicited the production of several inflammation-related cytokines, while DPPC/CHOL- or DSPC/CHOL-functionalized liposomes did not. This work underlines the need for accurate characterization at multiple levels and the use of reliable in vitro methods, in order to obtain a realistic assessment of liposome-induced human inflammatory response, as a fundamental requirement of nanosafety regulations. |
| first_indexed | 2024-03-09T04:40:59Z |
| format | Article |
| id | doaj.art-665d502e4eed4f6e96b623eaa7e02397 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T04:40:59Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-665d502e4eed4f6e96b623eaa7e023972023-12-03T13:21:02ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-01-0122282010.3390/ijms22020820A Step-by-Step Approach to Improve Clinical Translation of Liposome-Based Nanomaterials, a Focus on Innate Immune and Inflammatory ResponsesGiacomo Della Camera0Dorelia Lipsa1Dora Mehn2Paola Italiani3Diana Boraschi4Sabrina Gioria5European Commission, Joint Research Centre (JRC), 21027 Ispra, ItalyEuropean Commission, Joint Research Centre (JRC), 21027 Ispra, ItalyEuropean Commission, Joint Research Centre (JRC), 21027 Ispra, ItalyInstitute of Biochemistry and Cell Biology (IBBC), National Research Council (CNR), 80131 Naples, ItalyInstitute of Biochemistry and Cell Biology (IBBC), National Research Council (CNR), 80131 Naples, ItalyEuropean Commission, Joint Research Centre (JRC), 21027 Ispra, ItalyThis study aims to provide guidelines to design and perform a robust and reliable physical-chemical characterization of liposome-based nanomaterials, and to support method development with a specific focus on their inflammation-inducing potential. Out of eight differently functionalized liposomes selected as “case-studies”, three passed the physical-chemical characterization (in terms of size-distribution, homogeneity and stability) and the screening for bacterial contamination (sterility and apyrogenicity). Although all three were non-cytotoxic when tested in vitro, they showed a different capacity to activate human blood cells. HSPC/CHOL-coated liposomes elicited the production of several inflammation-related cytokines, while DPPC/CHOL- or DSPC/CHOL-functionalized liposomes did not. This work underlines the need for accurate characterization at multiple levels and the use of reliable in vitro methods, in order to obtain a realistic assessment of liposome-induced human inflammatory response, as a fundamental requirement of nanosafety regulations.https://www.mdpi.com/1422-0067/22/2/820nanomaterialliposomesafety assessmentnanomedicineparticle size distributionphysicochemical characterization |
| spellingShingle | Giacomo Della Camera Dorelia Lipsa Dora Mehn Paola Italiani Diana Boraschi Sabrina Gioria A Step-by-Step Approach to Improve Clinical Translation of Liposome-Based Nanomaterials, a Focus on Innate Immune and Inflammatory Responses International Journal of Molecular Sciences nanomaterial liposome safety assessment nanomedicine particle size distribution physicochemical characterization |
| title | A Step-by-Step Approach to Improve Clinical Translation of Liposome-Based Nanomaterials, a Focus on Innate Immune and Inflammatory Responses |
| title_full | A Step-by-Step Approach to Improve Clinical Translation of Liposome-Based Nanomaterials, a Focus on Innate Immune and Inflammatory Responses |
| title_fullStr | A Step-by-Step Approach to Improve Clinical Translation of Liposome-Based Nanomaterials, a Focus on Innate Immune and Inflammatory Responses |
| title_full_unstemmed | A Step-by-Step Approach to Improve Clinical Translation of Liposome-Based Nanomaterials, a Focus on Innate Immune and Inflammatory Responses |
| title_short | A Step-by-Step Approach to Improve Clinical Translation of Liposome-Based Nanomaterials, a Focus on Innate Immune and Inflammatory Responses |
| title_sort | step by step approach to improve clinical translation of liposome based nanomaterials a focus on innate immune and inflammatory responses |
| topic | nanomaterial liposome safety assessment nanomedicine particle size distribution physicochemical characterization |
| url | https://www.mdpi.com/1422-0067/22/2/820 |
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