KANPHOS: A Database of Kinase-Associated Neural Protein Phosphorylation in the Brain
Protein phosphorylation plays critical roles in a variety of intracellular signaling pathways and physiological functions that are controlled by neurotransmitters and neuromodulators in the brain. Dysregulation of these signaling pathways has been implicated in neurodevelopmental disorders, includin...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-12-01
|
| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/11/1/47 |
| _version_ | 1827668626547671040 |
|---|---|
| author | Rijwan Uddin Ahammad Tomoki Nishioka Junichiro Yoshimoto Takayuki Kannon Mutsuki Amano Yasuhiro Funahashi Daisuke Tsuboi Md. Omar Faruk Yukie Yamahashi Kiyofumi Yamada Taku Nagai Kozo Kaibuchi |
| author_facet | Rijwan Uddin Ahammad Tomoki Nishioka Junichiro Yoshimoto Takayuki Kannon Mutsuki Amano Yasuhiro Funahashi Daisuke Tsuboi Md. Omar Faruk Yukie Yamahashi Kiyofumi Yamada Taku Nagai Kozo Kaibuchi |
| author_sort | Rijwan Uddin Ahammad |
| collection | DOAJ |
| description | Protein phosphorylation plays critical roles in a variety of intracellular signaling pathways and physiological functions that are controlled by neurotransmitters and neuromodulators in the brain. Dysregulation of these signaling pathways has been implicated in neurodevelopmental disorders, including autism spectrum disorder, attention deficit hyperactivity disorder and schizophrenia. While recent advances in mass spectrometry-based proteomics have allowed us to identify approximately 280,000 phosphorylation sites, it remains largely unknown which sites are phosphorylated by which kinases. To overcome this issue, previously, we developed methods for comprehensive screening of the target substrates of given kinases, such as PKA and Rho-kinase, upon stimulation by extracellular signals and identified many candidate substrates for specific kinases and their phosphorylation sites. Here, we developed a novel online database to provide information about the phosphorylation signals identified by our methods, as well as those previously reported in the literature. The “KANPHOS” (Kinase-Associated Neural Phospho-Signaling) database and its web portal were built based on a next-generation XooNIps neuroinformatics tool. To explore the functionality of the KANPHOS database, we obtained phosphoproteomics data for adenosine-A2A-receptor signaling and its downstream MAPK-mediated signaling in the striatum/nucleus accumbens, registered them in KANPHOS, and analyzed the related pathways. |
| first_indexed | 2024-03-10T03:46:22Z |
| format | Article |
| id | doaj.art-665e07ff34fe4f8993088c7675ccf2ee |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T03:46:22Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-665e07ff34fe4f8993088c7675ccf2ee2023-11-23T11:19:22ZengMDPI AGCells2073-44092021-12-011114710.3390/cells11010047KANPHOS: A Database of Kinase-Associated Neural Protein Phosphorylation in the BrainRijwan Uddin Ahammad0Tomoki Nishioka1Junichiro Yoshimoto2Takayuki Kannon3Mutsuki Amano4Yasuhiro Funahashi5Daisuke Tsuboi6Md. Omar Faruk7Yukie Yamahashi8Kiyofumi Yamada9Taku Nagai10Kozo Kaibuchi11Department of Cell Pharmacology, Graduate School of Medicine, Nagoya University, 65 Tsurumai, Nagoya 466-8550, JapanInstitute for Comprehensive Medical Science, Fujita Health University, Toyoake 470-1192, JapanDivision of Information Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, Ikoma 630-0192, JapanDepartment of Bioinformatics and Genomics, Graduate School of Advanced Preventive Medical Science, Kanazawa University, Kanazawa 920-8640, JapanDepartment of Cell Pharmacology, Graduate School of Medicine, Nagoya University, 65 Tsurumai, Nagoya 466-8550, JapanInstitute for Comprehensive Medical Science, Fujita Health University, Toyoake 470-1192, JapanInstitute for Comprehensive Medical Science, Fujita Health University, Toyoake 470-1192, JapanDepartment of Cell Pharmacology, Graduate School of Medicine, Nagoya University, 65 Tsurumai, Nagoya 466-8550, JapanInstitute for Comprehensive Medical Science, Fujita Health University, Toyoake 470-1192, JapanDepartment of Neuropsychopharmacology and Hospital Pharmacy, Graduate School of Medicine, Nagoya University, 65 Tsurumai, Nagoya 466-8550, JapanDivision of Behavioral Neuropharmacology, International Center for Brain Science (ICBS), Fujita Health University, Toyoake 470-1192, JapanDepartment of Cell Pharmacology, Graduate School of Medicine, Nagoya University, 65 Tsurumai, Nagoya 466-8550, JapanProtein phosphorylation plays critical roles in a variety of intracellular signaling pathways and physiological functions that are controlled by neurotransmitters and neuromodulators in the brain. Dysregulation of these signaling pathways has been implicated in neurodevelopmental disorders, including autism spectrum disorder, attention deficit hyperactivity disorder and schizophrenia. While recent advances in mass spectrometry-based proteomics have allowed us to identify approximately 280,000 phosphorylation sites, it remains largely unknown which sites are phosphorylated by which kinases. To overcome this issue, previously, we developed methods for comprehensive screening of the target substrates of given kinases, such as PKA and Rho-kinase, upon stimulation by extracellular signals and identified many candidate substrates for specific kinases and their phosphorylation sites. Here, we developed a novel online database to provide information about the phosphorylation signals identified by our methods, as well as those previously reported in the literature. The “KANPHOS” (Kinase-Associated Neural Phospho-Signaling) database and its web portal were built based on a next-generation XooNIps neuroinformatics tool. To explore the functionality of the KANPHOS database, we obtained phosphoproteomics data for adenosine-A2A-receptor signaling and its downstream MAPK-mediated signaling in the striatum/nucleus accumbens, registered them in KANPHOS, and analyzed the related pathways.https://www.mdpi.com/2073-4409/11/1/47KANPHOSphosphorylationphosphoproteomicssignal transductionmolecular mechanismadenosine signaling |
| spellingShingle | Rijwan Uddin Ahammad Tomoki Nishioka Junichiro Yoshimoto Takayuki Kannon Mutsuki Amano Yasuhiro Funahashi Daisuke Tsuboi Md. Omar Faruk Yukie Yamahashi Kiyofumi Yamada Taku Nagai Kozo Kaibuchi KANPHOS: A Database of Kinase-Associated Neural Protein Phosphorylation in the Brain Cells KANPHOS phosphorylation phosphoproteomics signal transduction molecular mechanism adenosine signaling |
| title | KANPHOS: A Database of Kinase-Associated Neural Protein Phosphorylation in the Brain |
| title_full | KANPHOS: A Database of Kinase-Associated Neural Protein Phosphorylation in the Brain |
| title_fullStr | KANPHOS: A Database of Kinase-Associated Neural Protein Phosphorylation in the Brain |
| title_full_unstemmed | KANPHOS: A Database of Kinase-Associated Neural Protein Phosphorylation in the Brain |
| title_short | KANPHOS: A Database of Kinase-Associated Neural Protein Phosphorylation in the Brain |
| title_sort | kanphos a database of kinase associated neural protein phosphorylation in the brain |
| topic | KANPHOS phosphorylation phosphoproteomics signal transduction molecular mechanism adenosine signaling |
| url | https://www.mdpi.com/2073-4409/11/1/47 |
| work_keys_str_mv | AT rijwanuddinahammad kanphosadatabaseofkinaseassociatedneuralproteinphosphorylationinthebrain AT tomokinishioka kanphosadatabaseofkinaseassociatedneuralproteinphosphorylationinthebrain AT junichiroyoshimoto kanphosadatabaseofkinaseassociatedneuralproteinphosphorylationinthebrain AT takayukikannon kanphosadatabaseofkinaseassociatedneuralproteinphosphorylationinthebrain AT mutsukiamano kanphosadatabaseofkinaseassociatedneuralproteinphosphorylationinthebrain AT yasuhirofunahashi kanphosadatabaseofkinaseassociatedneuralproteinphosphorylationinthebrain AT daisuketsuboi kanphosadatabaseofkinaseassociatedneuralproteinphosphorylationinthebrain AT mdomarfaruk kanphosadatabaseofkinaseassociatedneuralproteinphosphorylationinthebrain AT yukieyamahashi kanphosadatabaseofkinaseassociatedneuralproteinphosphorylationinthebrain AT kiyofumiyamada kanphosadatabaseofkinaseassociatedneuralproteinphosphorylationinthebrain AT takunagai kanphosadatabaseofkinaseassociatedneuralproteinphosphorylationinthebrain AT kozokaibuchi kanphosadatabaseofkinaseassociatedneuralproteinphosphorylationinthebrain |