Genetic strategies to investigate neuronal circuit properties using stem cell-derived neurons
The mammalian brain is anatomically and functionally complex, and prone to diverse forms of injury and neuropathology. Scientists have long strived to develop cell replacement therapies to repair damaged and diseased nervous tissue. However, this goal has remained unrealized for various reasons, i...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2012-12-01
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| Series: | Frontiers in Cellular Neuroscience |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fncel.2012.00059/full |
| _version_ | 1818494094169604096 |
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| author | Isabella eGarcia Cynthia eKim Benjamin R. Arenkiel |
| author_facet | Isabella eGarcia Cynthia eKim Benjamin R. Arenkiel |
| author_sort | Isabella eGarcia |
| collection | DOAJ |
| description | The mammalian brain is anatomically and functionally complex, and prone to diverse forms of injury and neuropathology. Scientists have long strived to develop cell replacement therapies to repair damaged and diseased nervous tissue. However, this goal has remained unrealized for various reasons, including nascent knowledge of neuronal development, the inability to track and manipulate transplanted cells within complex neuronal networks, and host graft rejection. Recent advances in embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) technology, alongside novel genetic strategies to mark and manipulate stem cell-derived neurons now provide unprecedented opportunities to investigate complex neuronal circuits in both healthy and diseased brains. Here, we review current technologies aimed at generating and manipulating neurons derived from ESCs and iPSCs towards investigation and manipulation of complex neuronal circuits, ultimately leading to the design and development of novel cell-based therapeutic approaches. |
| first_indexed | 2024-12-10T18:02:11Z |
| format | Article |
| id | doaj.art-6664ab8442784ff9ba85353c6f45ecb5 |
| institution | Directory Open Access Journal |
| issn | 1662-5102 |
| language | English |
| last_indexed | 2024-12-10T18:02:11Z |
| publishDate | 2012-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular Neuroscience |
| spelling | doaj.art-6664ab8442784ff9ba85353c6f45ecb52022-12-22T01:38:44ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022012-12-01610.3389/fncel.2012.0005939328Genetic strategies to investigate neuronal circuit properties using stem cell-derived neuronsIsabella eGarcia0Cynthia eKim1Benjamin R. Arenkiel2Baylor College of MedicineBaylor College of MedicineBaylor College of MedicineThe mammalian brain is anatomically and functionally complex, and prone to diverse forms of injury and neuropathology. Scientists have long strived to develop cell replacement therapies to repair damaged and diseased nervous tissue. However, this goal has remained unrealized for various reasons, including nascent knowledge of neuronal development, the inability to track and manipulate transplanted cells within complex neuronal networks, and host graft rejection. Recent advances in embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) technology, alongside novel genetic strategies to mark and manipulate stem cell-derived neurons now provide unprecedented opportunities to investigate complex neuronal circuits in both healthy and diseased brains. Here, we review current technologies aimed at generating and manipulating neurons derived from ESCs and iPSCs towards investigation and manipulation of complex neuronal circuits, ultimately leading to the design and development of novel cell-based therapeutic approaches.http://journal.frontiersin.org/Journal/10.3389/fncel.2012.00059/fullGenetic EngineeringStem CellsTransplantationneural circuitsoptogeneticsneuronal differentiation |
| spellingShingle | Isabella eGarcia Cynthia eKim Benjamin R. Arenkiel Genetic strategies to investigate neuronal circuit properties using stem cell-derived neurons Frontiers in Cellular Neuroscience Genetic Engineering Stem Cells Transplantation neural circuits optogenetics neuronal differentiation |
| title | Genetic strategies to investigate neuronal circuit properties using stem cell-derived neurons |
| title_full | Genetic strategies to investigate neuronal circuit properties using stem cell-derived neurons |
| title_fullStr | Genetic strategies to investigate neuronal circuit properties using stem cell-derived neurons |
| title_full_unstemmed | Genetic strategies to investigate neuronal circuit properties using stem cell-derived neurons |
| title_short | Genetic strategies to investigate neuronal circuit properties using stem cell-derived neurons |
| title_sort | genetic strategies to investigate neuronal circuit properties using stem cell derived neurons |
| topic | Genetic Engineering Stem Cells Transplantation neural circuits optogenetics neuronal differentiation |
| url | http://journal.frontiersin.org/Journal/10.3389/fncel.2012.00059/full |
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