PLC1 mediated Cycloastragenol-induced stomatal movement by regulating the production of NO in Arabidopsis thaliana
Abstract Background Astragalus grows mainly in drought areas. Cycloastragenol (CAG) is a tetracyclic triterpenoid allelochemical extracted from traditional Chinese medicine Astragalus root. Phospholipase C (PLC) and Gα-submit of the heterotrimeric G-protein (GPA1) are involved in many biotic or abio...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-11-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-023-04555-7 |
| _version_ | 1827723690008117248 |
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| author | Juantao Kong Rongshan Chen Ruirui Liu Wei Wang Simin Wang Jinping Zhang Ning Yang |
| author_facet | Juantao Kong Rongshan Chen Ruirui Liu Wei Wang Simin Wang Jinping Zhang Ning Yang |
| author_sort | Juantao Kong |
| collection | DOAJ |
| description | Abstract Background Astragalus grows mainly in drought areas. Cycloastragenol (CAG) is a tetracyclic triterpenoid allelochemical extracted from traditional Chinese medicine Astragalus root. Phospholipase C (PLC) and Gα-submit of the heterotrimeric G-protein (GPA1) are involved in many biotic or abiotic stresses. Nitric oxide (NO) is a crucial gas signal molecule in plants. Results In this study, using the seedlings of Arabidopsis thaliana (A. thaliana), the results showed that low concentrations of CAG induced stomatal closure, and high concentrations inhibited stomatal closure. 30 µmol·L−1 CAG significantly increased the relative expression levels of PLC1 and GPA1 and the activities of PLC and GTP hydrolysis. The stomatal aperture of plc1, gpa1, and plc1/gpa1 was higher than that of WT under CAG treatment. CAG increased the fluorescence intensity of NO in guard cells. Exogenous application of c-PTIO to WT significantly induced stomatal aperture under CAG treatment. CAG significantly increased the relative expression levels of NIA1 and NOA1. Mutants of noa1, nia1, and nia2 showed that NO production was mainly from NOA1 and NIA1 by CAG treatment. The fluorescence intensity of NO in guard cells of plc1, gpa1, and plc1/gpa1 was lower than WT, indicating that PLC1 and GPA1 were involved in the NO production in guard cells. There was no significant difference in the gene expression of PLC1 in WT, nia1, and noa1 under CAG treatment. The gene expression levels of NIA1 and NOA1 in plc1, gpa1, and plc1/gpa1 were significantly lower than WT, indicating that PLC1 and GPA1 were positively regulating NO production by regulating the expression of NIA1 and NOA1 under CAG treatment. Conclusions These results suggested that the NO accumulation was essential to induce stomatal closure under CAG treatment, and GPA1 and PLC1 acted upstream of NO. |
| first_indexed | 2024-03-10T22:03:20Z |
| format | Article |
| id | doaj.art-66663cbd85f6440cbb985f6b47ac0cf2 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-03-10T22:03:20Z |
| publishDate | 2023-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-66663cbd85f6440cbb985f6b47ac0cf22023-11-19T12:52:37ZengBMCBMC Plant Biology1471-22292023-11-0123111110.1186/s12870-023-04555-7PLC1 mediated Cycloastragenol-induced stomatal movement by regulating the production of NO in Arabidopsis thalianaJuantao Kong0Rongshan Chen1Ruirui Liu2Wei Wang3Simin Wang4Jinping Zhang5Ning Yang6College of Life Science, Northwest Normal UniversityCollege of Life Science, Northwest Normal UniversityCollege of Life Science, Northwest Normal UniversityCollege of Life Science, Northwest Normal UniversityCollege of Life Science, Northwest Normal UniversityCollege of Life Science, Northwest Normal UniversityCollege of Life Science, Northwest Normal UniversityAbstract Background Astragalus grows mainly in drought areas. Cycloastragenol (CAG) is a tetracyclic triterpenoid allelochemical extracted from traditional Chinese medicine Astragalus root. Phospholipase C (PLC) and Gα-submit of the heterotrimeric G-protein (GPA1) are involved in many biotic or abiotic stresses. Nitric oxide (NO) is a crucial gas signal molecule in plants. Results In this study, using the seedlings of Arabidopsis thaliana (A. thaliana), the results showed that low concentrations of CAG induced stomatal closure, and high concentrations inhibited stomatal closure. 30 µmol·L−1 CAG significantly increased the relative expression levels of PLC1 and GPA1 and the activities of PLC and GTP hydrolysis. The stomatal aperture of plc1, gpa1, and plc1/gpa1 was higher than that of WT under CAG treatment. CAG increased the fluorescence intensity of NO in guard cells. Exogenous application of c-PTIO to WT significantly induced stomatal aperture under CAG treatment. CAG significantly increased the relative expression levels of NIA1 and NOA1. Mutants of noa1, nia1, and nia2 showed that NO production was mainly from NOA1 and NIA1 by CAG treatment. The fluorescence intensity of NO in guard cells of plc1, gpa1, and plc1/gpa1 was lower than WT, indicating that PLC1 and GPA1 were involved in the NO production in guard cells. There was no significant difference in the gene expression of PLC1 in WT, nia1, and noa1 under CAG treatment. The gene expression levels of NIA1 and NOA1 in plc1, gpa1, and plc1/gpa1 were significantly lower than WT, indicating that PLC1 and GPA1 were positively regulating NO production by regulating the expression of NIA1 and NOA1 under CAG treatment. Conclusions These results suggested that the NO accumulation was essential to induce stomatal closure under CAG treatment, and GPA1 and PLC1 acted upstream of NO.https://doi.org/10.1186/s12870-023-04555-7CycloastragenolStomatal movementPhospholipase C1Gα-submit of the heterotrimeric G-proteinNitric oxide |
| spellingShingle | Juantao Kong Rongshan Chen Ruirui Liu Wei Wang Simin Wang Jinping Zhang Ning Yang PLC1 mediated Cycloastragenol-induced stomatal movement by regulating the production of NO in Arabidopsis thaliana BMC Plant Biology Cycloastragenol Stomatal movement Phospholipase C1 Gα-submit of the heterotrimeric G-protein Nitric oxide |
| title | PLC1 mediated Cycloastragenol-induced stomatal movement by regulating the production of NO in Arabidopsis thaliana |
| title_full | PLC1 mediated Cycloastragenol-induced stomatal movement by regulating the production of NO in Arabidopsis thaliana |
| title_fullStr | PLC1 mediated Cycloastragenol-induced stomatal movement by regulating the production of NO in Arabidopsis thaliana |
| title_full_unstemmed | PLC1 mediated Cycloastragenol-induced stomatal movement by regulating the production of NO in Arabidopsis thaliana |
| title_short | PLC1 mediated Cycloastragenol-induced stomatal movement by regulating the production of NO in Arabidopsis thaliana |
| title_sort | plc1 mediated cycloastragenol induced stomatal movement by regulating the production of no in arabidopsis thaliana |
| topic | Cycloastragenol Stomatal movement Phospholipase C1 Gα-submit of the heterotrimeric G-protein Nitric oxide |
| url | https://doi.org/10.1186/s12870-023-04555-7 |
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