A Six-Day, Lifestyle-Based Immersion Program Mitigates Cardiovascular Risk Factors and Induces Shifts in Gut Microbiota, Specifically <i>Lachnospiraceae</i>, <i>Ruminococcaceae</i>, <i>Faecalibacterium prausnitzii</i>: A Pilot Study
Cardiovascular disease (CVD) prevalence remains elevated globally. We have previously shown that a one-week lifestyle “immersion program” leads to clinical improvements and sustained improvements in quality of life in moderate to high atherosclerotic CVD (ASCVD) risk individuals. In a subsequent yea...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/13/10/3459 |
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| author | Angelica P. Ahrens Tyler Culpepper Brittany Saldivar Stephen Anton Scott Stoll Eileen M. Handberg Ke Xu Carl Pepine Eric W. Triplett Monica Aggarwal |
| author_facet | Angelica P. Ahrens Tyler Culpepper Brittany Saldivar Stephen Anton Scott Stoll Eileen M. Handberg Ke Xu Carl Pepine Eric W. Triplett Monica Aggarwal |
| author_sort | Angelica P. Ahrens |
| collection | DOAJ |
| description | Cardiovascular disease (CVD) prevalence remains elevated globally. We have previously shown that a one-week lifestyle “immersion program” leads to clinical improvements and sustained improvements in quality of life in moderate to high atherosclerotic CVD (ASCVD) risk individuals. In a subsequent year of this similarly modeled immersion program, we again collected markers of cardiovascular health and, additionally, evaluated intestinal microbiome composition. ASCVD risk volunteers (<i>n</i> = 73) completed the one-week “immersion program” involving nutrition (100% plant-based foods), stress management education, and exercise. Anthropometric measurements and CVD risk factors were compared at baseline and post intervention. A subgroup (<i>n</i> = 22) provided stool, which we analyzed with 16S rRNA sequencing. We assessed abundance changes within-person, correlated the abundance shifts with clinical changes, and inferred functional pathways using PICRUSt. Reductions in blood pressure, total cholesterol, and triglycerides, were observed without reduction in weight. Significant increases in butyrate producers were detected, including <i>Lachnospiraceae</i> and <i>Oscillospirales</i>. Within-person, significant shifts in relative abundance (RA) occurred, e.g., increased <i>Lachnospiraceae</i> (+58.8% RA, <i>p</i> = 0.0002), <i>Ruminococcaceae</i> (+82.1%, <i>p</i> = 0.0003), <i>Faecalibacterium prausnitzii</i> (+54.5%, <i>p</i> = 0.002), and diversification and richness. Microbiota changes significantly correlated with body mass index (BMI), blood pressure (BP), cholesterol, high-sensitivity C-reactive protein (hsCRP), glucose, and trimethylamine N-oxide (TMAO) changes. Pairwise decreases were inferred in microbial genes corresponding to cancer, metabolic disease, and amino acid metabolism. This brief lifestyle-based intervention improved lipids and BP and enhanced known butyrate producers, without significant weight loss. These results demonstrate a promising non-pharmacological preventative strategy for improving cardiovascular health. |
| first_indexed | 2024-03-10T06:18:11Z |
| format | Article |
| id | doaj.art-6667eed6a44d483eb6c873b6f3e60dd4 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T06:18:11Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-6667eed6a44d483eb6c873b6f3e60dd42023-11-22T19:28:52ZengMDPI AGNutrients2072-66432021-09-011310345910.3390/nu13103459A Six-Day, Lifestyle-Based Immersion Program Mitigates Cardiovascular Risk Factors and Induces Shifts in Gut Microbiota, Specifically <i>Lachnospiraceae</i>, <i>Ruminococcaceae</i>, <i>Faecalibacterium prausnitzii</i>: A Pilot StudyAngelica P. Ahrens0Tyler Culpepper1Brittany Saldivar2Stephen Anton3Scott Stoll4Eileen M. Handberg5Ke Xu6Carl Pepine7Eric W. Triplett8Monica Aggarwal9Department of Microbiology and Cell Science, College of Agricultural and Life Sciences, University of Florida, 1355 Museum Dr, Gainesville, FL 32603, USADepartment of Medicine, College of Medicine, University of Florida, P.O. Box 100277, Gainesville, FL 32610, USADepartment of Medicine, College of Medicine, University of Florida, P.O. Box 100277, Gainesville, FL 32610, USADepartment of Aging and Geriatric Research, University of Florida, 210 E. Mowry Rd, Gainesville, FL 32611, USATotal Health Immersions, P.O. Box 741596, Boynton Beach, FL 33474, USADivision of Cardiovascular Medicine, Department of Medicine, University of Florida, 1600 SW Archer Rd, Gainesville, FL 32610, USADepartment of Health Outcomes and Biomedical Informatics, College of Medicine, University of Florida, 2004 Mowry Rd, Gainesville, FL 32610, USADivision of Cardiovascular Medicine, Department of Medicine, University of Florida, 1600 SW Archer Rd, Gainesville, FL 32610, USADepartment of Microbiology and Cell Science, College of Agricultural and Life Sciences, University of Florida, 1355 Museum Dr, Gainesville, FL 32603, USADivision of Cardiovascular Medicine, Department of Medicine, University of Florida, 1600 SW Archer Rd, Gainesville, FL 32610, USACardiovascular disease (CVD) prevalence remains elevated globally. We have previously shown that a one-week lifestyle “immersion program” leads to clinical improvements and sustained improvements in quality of life in moderate to high atherosclerotic CVD (ASCVD) risk individuals. In a subsequent year of this similarly modeled immersion program, we again collected markers of cardiovascular health and, additionally, evaluated intestinal microbiome composition. ASCVD risk volunteers (<i>n</i> = 73) completed the one-week “immersion program” involving nutrition (100% plant-based foods), stress management education, and exercise. Anthropometric measurements and CVD risk factors were compared at baseline and post intervention. A subgroup (<i>n</i> = 22) provided stool, which we analyzed with 16S rRNA sequencing. We assessed abundance changes within-person, correlated the abundance shifts with clinical changes, and inferred functional pathways using PICRUSt. Reductions in blood pressure, total cholesterol, and triglycerides, were observed without reduction in weight. Significant increases in butyrate producers were detected, including <i>Lachnospiraceae</i> and <i>Oscillospirales</i>. Within-person, significant shifts in relative abundance (RA) occurred, e.g., increased <i>Lachnospiraceae</i> (+58.8% RA, <i>p</i> = 0.0002), <i>Ruminococcaceae</i> (+82.1%, <i>p</i> = 0.0003), <i>Faecalibacterium prausnitzii</i> (+54.5%, <i>p</i> = 0.002), and diversification and richness. Microbiota changes significantly correlated with body mass index (BMI), blood pressure (BP), cholesterol, high-sensitivity C-reactive protein (hsCRP), glucose, and trimethylamine N-oxide (TMAO) changes. Pairwise decreases were inferred in microbial genes corresponding to cancer, metabolic disease, and amino acid metabolism. This brief lifestyle-based intervention improved lipids and BP and enhanced known butyrate producers, without significant weight loss. These results demonstrate a promising non-pharmacological preventative strategy for improving cardiovascular health.https://www.mdpi.com/2072-6643/13/10/3459hypertensioncholesterollifestyleinflammationwhole foodplant based diet |
| spellingShingle | Angelica P. Ahrens Tyler Culpepper Brittany Saldivar Stephen Anton Scott Stoll Eileen M. Handberg Ke Xu Carl Pepine Eric W. Triplett Monica Aggarwal A Six-Day, Lifestyle-Based Immersion Program Mitigates Cardiovascular Risk Factors and Induces Shifts in Gut Microbiota, Specifically <i>Lachnospiraceae</i>, <i>Ruminococcaceae</i>, <i>Faecalibacterium prausnitzii</i>: A Pilot Study Nutrients hypertension cholesterol lifestyle inflammation whole food plant based diet |
| title | A Six-Day, Lifestyle-Based Immersion Program Mitigates Cardiovascular Risk Factors and Induces Shifts in Gut Microbiota, Specifically <i>Lachnospiraceae</i>, <i>Ruminococcaceae</i>, <i>Faecalibacterium prausnitzii</i>: A Pilot Study |
| title_full | A Six-Day, Lifestyle-Based Immersion Program Mitigates Cardiovascular Risk Factors and Induces Shifts in Gut Microbiota, Specifically <i>Lachnospiraceae</i>, <i>Ruminococcaceae</i>, <i>Faecalibacterium prausnitzii</i>: A Pilot Study |
| title_fullStr | A Six-Day, Lifestyle-Based Immersion Program Mitigates Cardiovascular Risk Factors and Induces Shifts in Gut Microbiota, Specifically <i>Lachnospiraceae</i>, <i>Ruminococcaceae</i>, <i>Faecalibacterium prausnitzii</i>: A Pilot Study |
| title_full_unstemmed | A Six-Day, Lifestyle-Based Immersion Program Mitigates Cardiovascular Risk Factors and Induces Shifts in Gut Microbiota, Specifically <i>Lachnospiraceae</i>, <i>Ruminococcaceae</i>, <i>Faecalibacterium prausnitzii</i>: A Pilot Study |
| title_short | A Six-Day, Lifestyle-Based Immersion Program Mitigates Cardiovascular Risk Factors and Induces Shifts in Gut Microbiota, Specifically <i>Lachnospiraceae</i>, <i>Ruminococcaceae</i>, <i>Faecalibacterium prausnitzii</i>: A Pilot Study |
| title_sort | six day lifestyle based immersion program mitigates cardiovascular risk factors and induces shifts in gut microbiota specifically i lachnospiraceae i i ruminococcaceae i i faecalibacterium prausnitzii i a pilot study |
| topic | hypertension cholesterol lifestyle inflammation whole food plant based diet |
| url | https://www.mdpi.com/2072-6643/13/10/3459 |
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