A Novel Immunobiotics Bacteroides dorei Ameliorates Influenza Virus Infection in Mice
ObjectiveProbiotics can modulate immune responses to resist influenza infection. This study aims to evaluate the anti-viral efficacy of B. dorei.MethodsC57BL/6J mice were infected with influenza virus together with treatment of PBS vehicle, B. dorei, or oseltamivir respectively. Anti-influenza poten...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-01-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2021.828887/full |
| _version_ | 1798025010042896384 |
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| author | Liqiong Song Yuanming Huang Guoxing Liu Guoxing Liu Xianping Li Yuchun Xiao Chang Liu Yue Zhang Jintong Li Jianguo Xu Shan Lu Zhihong Ren |
| author_facet | Liqiong Song Yuanming Huang Guoxing Liu Guoxing Liu Xianping Li Yuchun Xiao Chang Liu Yue Zhang Jintong Li Jianguo Xu Shan Lu Zhihong Ren |
| author_sort | Liqiong Song |
| collection | DOAJ |
| description | ObjectiveProbiotics can modulate immune responses to resist influenza infection. This study aims to evaluate the anti-viral efficacy of B. dorei.MethodsC57BL/6J mice were infected with influenza virus together with treatment of PBS vehicle, B. dorei, or oseltamivir respectively. Anti-influenza potency of B. dorei and the underlying mechanism were determined by measuring survival rate, lung viral load and pathology, gene expression and production of cytokines and chemokines, and analysis of gut microbiota.ResultsAdministration of B. dorei increased (by 30%) the survival of influenza-infected mice, and improved their weight loss, lung pathology, lung index, and colon length compared to the vehicle control group. B. dorei treatment reduced (by 61%) the viral load of lung tissue and increased expression of type 1 interferon more rapidly at day 3 postinfection. At day 7 postinfection, B. dorei-treated mice showed lower local (lung) and systemic (serum) levels of interferon and several proinflammatory cytokines or chemokines (IL-1β, IL-6, TNF-α, IL-10, MCP-1 and IP-10) with a efficacy comparable to oseltamivi treatment. B. dorei treatment also altered gut microbiota as indicated by increased levels of Bacteroides, Prevotella, and Lactobacillus and decreased levels of Escherichia, Shigella, and Parabacteroides.ConclusionB. dorei has anti-influenza effect. Its working mechanisms involve promoting earlier interferon expression and down-regulating both local and systemic inflammatory response. B. dorei changes the composition of gut microbiota, which may also contribute to its beneficial effects. |
| first_indexed | 2024-04-11T18:11:51Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-11T18:11:51Z |
| publishDate | 2022-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-6669704c8c454dcab08f1410491a6b9c2022-12-22T04:10:06ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-01-011210.3389/fimmu.2021.828887828887A Novel Immunobiotics Bacteroides dorei Ameliorates Influenza Virus Infection in MiceLiqiong Song0Yuanming Huang1Guoxing Liu2Guoxing Liu3Xianping Li4Yuchun Xiao5Chang Liu6Yue Zhang7Jintong Li8Jianguo Xu9Shan Lu10Zhihong Ren11State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Research Units of Discovery of Unknown Bacteria and Function (2018 RU010), Chinese Academy of Medical Sciences, Beijing, ChinaState Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Research Units of Discovery of Unknown Bacteria and Function (2018 RU010), Chinese Academy of Medical Sciences, Beijing, ChinaRespiratory Department, Beijing University of Chinese Medicine Third Affiliated Hospital, Beijing, ChinaTraditional Chinese Medicine Department, Linwei Liu Zunji Clinic of Traditional Chinese Medicine, Weinan, ChinaState Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Research Units of Discovery of Unknown Bacteria and Function (2018 RU010), Chinese Academy of Medical Sciences, Beijing, ChinaState Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Research Units of Discovery of Unknown Bacteria and Function (2018 RU010), Chinese Academy of Medical Sciences, Beijing, ChinaRespiratory Department, Beijing University of Chinese Medicine Third Affiliated Hospital, Beijing, ChinaRespiratory Department, Beijing University of Chinese Medicine Third Affiliated Hospital, Beijing, ChinaRespiratory Department, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, ChinaState Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Research Units of Discovery of Unknown Bacteria and Function (2018 RU010), Chinese Academy of Medical Sciences, Beijing, ChinaState Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Research Units of Discovery of Unknown Bacteria and Function (2018 RU010), Chinese Academy of Medical Sciences, Beijing, ChinaState Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Research Units of Discovery of Unknown Bacteria and Function (2018 RU010), Chinese Academy of Medical Sciences, Beijing, ChinaObjectiveProbiotics can modulate immune responses to resist influenza infection. This study aims to evaluate the anti-viral efficacy of B. dorei.MethodsC57BL/6J mice were infected with influenza virus together with treatment of PBS vehicle, B. dorei, or oseltamivir respectively. Anti-influenza potency of B. dorei and the underlying mechanism were determined by measuring survival rate, lung viral load and pathology, gene expression and production of cytokines and chemokines, and analysis of gut microbiota.ResultsAdministration of B. dorei increased (by 30%) the survival of influenza-infected mice, and improved their weight loss, lung pathology, lung index, and colon length compared to the vehicle control group. B. dorei treatment reduced (by 61%) the viral load of lung tissue and increased expression of type 1 interferon more rapidly at day 3 postinfection. At day 7 postinfection, B. dorei-treated mice showed lower local (lung) and systemic (serum) levels of interferon and several proinflammatory cytokines or chemokines (IL-1β, IL-6, TNF-α, IL-10, MCP-1 and IP-10) with a efficacy comparable to oseltamivi treatment. B. dorei treatment also altered gut microbiota as indicated by increased levels of Bacteroides, Prevotella, and Lactobacillus and decreased levels of Escherichia, Shigella, and Parabacteroides.ConclusionB. dorei has anti-influenza effect. Its working mechanisms involve promoting earlier interferon expression and down-regulating both local and systemic inflammatory response. B. dorei changes the composition of gut microbiota, which may also contribute to its beneficial effects.https://www.frontiersin.org/articles/10.3389/fimmu.2021.828887/fullB. doreiinfluenzainterferongut microbiotacytokines |
| spellingShingle | Liqiong Song Yuanming Huang Guoxing Liu Guoxing Liu Xianping Li Yuchun Xiao Chang Liu Yue Zhang Jintong Li Jianguo Xu Shan Lu Zhihong Ren A Novel Immunobiotics Bacteroides dorei Ameliorates Influenza Virus Infection in Mice Frontiers in Immunology B. dorei influenza interferon gut microbiota cytokines |
| title | A Novel Immunobiotics Bacteroides dorei Ameliorates Influenza Virus Infection in Mice |
| title_full | A Novel Immunobiotics Bacteroides dorei Ameliorates Influenza Virus Infection in Mice |
| title_fullStr | A Novel Immunobiotics Bacteroides dorei Ameliorates Influenza Virus Infection in Mice |
| title_full_unstemmed | A Novel Immunobiotics Bacteroides dorei Ameliorates Influenza Virus Infection in Mice |
| title_short | A Novel Immunobiotics Bacteroides dorei Ameliorates Influenza Virus Infection in Mice |
| title_sort | novel immunobiotics bacteroides dorei ameliorates influenza virus infection in mice |
| topic | B. dorei influenza interferon gut microbiota cytokines |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2021.828887/full |
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