Genome Sequence and Adaptation Analysis of the Human and Rice Pathogenic Strain <i>Burkholderia glumae</i> AU6208
<i>Burkholderia glumae</i> causes rice (<i>Oryza sativa</i>) bacterial panicle blight, which is an increasingly economically important disease worldwide. As the first <i>B. glumae</i> strain isolated from patients with chronic infections, AU6208 has been reported...
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MDPI AG
2021-01-01
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| author | Zhouqi Cui Sai Wang Kaleem Ullah Kakar Guanglin Xie Bin Li Gongyou Chen Bo Zhu |
| author_facet | Zhouqi Cui Sai Wang Kaleem Ullah Kakar Guanglin Xie Bin Li Gongyou Chen Bo Zhu |
| author_sort | Zhouqi Cui |
| collection | DOAJ |
| description | <i>Burkholderia glumae</i> causes rice (<i>Oryza sativa</i>) bacterial panicle blight, which is an increasingly economically important disease worldwide. As the first <i>B. glumae</i> strain isolated from patients with chronic infections, AU6208 has been reported as an opportunistic clinic pathogen. However, our understanding of the molecular mechanism underlying human pathogenesis by <i>B. glumae</i> remains rudimentary. In this study, we report the complete genome sequence of the human-isolated <i>B. glumae</i> strain AU6208 and compare this to the genome of the rice-pathogenic <i>B. glumae</i> type strain LMG 2196<sup>T</sup>. Analysis of the average nucleotide identity demonstrated 99.4% similarity between the human- and plant-pathogenic strains. However, the phenotypic results from this study suggest a history of niche adaptation and divergence. In particular, we found 44 genes were predicted to be horizontally transferred into AU6208, and most of these genes were upregulated in conditions that mimic clinical conditions. In these, the gene pair <i>sbn</i>AB encodes key enzymes in antibiotic biosynthesis. These results suggest that horizontal gene transfer in AU6208 may be responsible for selective advantages in its pathogenicity in humans. Our analysis of the AU6208 genome and comparison with that of LMG 2196<sup>T</sup> reveal the evolutionary signatures of <i>B. glumae</i> in the process of switching niches from plants to humans. |
| first_indexed | 2024-03-09T04:14:02Z |
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| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T04:14:02Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-666ac553d5894aa583a5621213be05b42023-12-03T13:56:46ZengMDPI AGPathogens2076-08172021-01-011028710.3390/pathogens10020087Genome Sequence and Adaptation Analysis of the Human and Rice Pathogenic Strain <i>Burkholderia glumae</i> AU6208Zhouqi Cui0Sai Wang1Kaleem Ullah Kakar2Guanglin Xie3Bin Li4Gongyou Chen5Bo Zhu6Key Laboratory of Urban Agriculture by Ministry of Agriculture of China, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaKey Laboratory of Urban Agriculture by Ministry of Agriculture of China, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaDepartment of Plant Pathology & Ecology, the Connecticut Agricultural Experiment Station, New Haven, CT 06511, USAState Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310027, ChinaState Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310027, ChinaKey Laboratory of Urban Agriculture by Ministry of Agriculture of China, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaKey Laboratory of Urban Agriculture by Ministry of Agriculture of China, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China<i>Burkholderia glumae</i> causes rice (<i>Oryza sativa</i>) bacterial panicle blight, which is an increasingly economically important disease worldwide. As the first <i>B. glumae</i> strain isolated from patients with chronic infections, AU6208 has been reported as an opportunistic clinic pathogen. However, our understanding of the molecular mechanism underlying human pathogenesis by <i>B. glumae</i> remains rudimentary. In this study, we report the complete genome sequence of the human-isolated <i>B. glumae</i> strain AU6208 and compare this to the genome of the rice-pathogenic <i>B. glumae</i> type strain LMG 2196<sup>T</sup>. Analysis of the average nucleotide identity demonstrated 99.4% similarity between the human- and plant-pathogenic strains. However, the phenotypic results from this study suggest a history of niche adaptation and divergence. In particular, we found 44 genes were predicted to be horizontally transferred into AU6208, and most of these genes were upregulated in conditions that mimic clinical conditions. In these, the gene pair <i>sbn</i>AB encodes key enzymes in antibiotic biosynthesis. These results suggest that horizontal gene transfer in AU6208 may be responsible for selective advantages in its pathogenicity in humans. Our analysis of the AU6208 genome and comparison with that of LMG 2196<sup>T</sup> reveal the evolutionary signatures of <i>B. glumae</i> in the process of switching niches from plants to humans.https://www.mdpi.com/2076-0817/10/2/87<i>Burkholderia glumae</i>bacterial panicle blightchronic granulomatouscomparative analysishorizontal gene transfer |
| spellingShingle | Zhouqi Cui Sai Wang Kaleem Ullah Kakar Guanglin Xie Bin Li Gongyou Chen Bo Zhu Genome Sequence and Adaptation Analysis of the Human and Rice Pathogenic Strain <i>Burkholderia glumae</i> AU6208 Pathogens <i>Burkholderia glumae</i> bacterial panicle blight chronic granulomatous comparative analysis horizontal gene transfer |
| title | Genome Sequence and Adaptation Analysis of the Human and Rice Pathogenic Strain <i>Burkholderia glumae</i> AU6208 |
| title_full | Genome Sequence and Adaptation Analysis of the Human and Rice Pathogenic Strain <i>Burkholderia glumae</i> AU6208 |
| title_fullStr | Genome Sequence and Adaptation Analysis of the Human and Rice Pathogenic Strain <i>Burkholderia glumae</i> AU6208 |
| title_full_unstemmed | Genome Sequence and Adaptation Analysis of the Human and Rice Pathogenic Strain <i>Burkholderia glumae</i> AU6208 |
| title_short | Genome Sequence and Adaptation Analysis of the Human and Rice Pathogenic Strain <i>Burkholderia glumae</i> AU6208 |
| title_sort | genome sequence and adaptation analysis of the human and rice pathogenic strain i burkholderia glumae i au6208 |
| topic | <i>Burkholderia glumae</i> bacterial panicle blight chronic granulomatous comparative analysis horizontal gene transfer |
| url | https://www.mdpi.com/2076-0817/10/2/87 |
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