Cell-Free Supernatant (CFS) from <i>Bacillus subtilis</i> EB2004S and <i>Lactobacillus helveticus</i> EL2006H Cultured at a Range of pH Levels Modulates Potato Plant Growth under Greenhouse Conditions
Agriculture involving industrial fertilizers is another major human made contributing factor to soil pH variation after natural factors such as soil parent rock, weathering time span, climate, and vegetation. The current study assessed the potential effect of cell-free supernatant (CFS) obtained fro...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-04-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/7/6620 |
| _version_ | 1797607730424315904 |
|---|---|
| author | Levini A. Msimbira Judith Naamala Sowmyalakshmi Subramanian Donald L. Smith |
| author_facet | Levini A. Msimbira Judith Naamala Sowmyalakshmi Subramanian Donald L. Smith |
| author_sort | Levini A. Msimbira |
| collection | DOAJ |
| description | Agriculture involving industrial fertilizers is another major human made contributing factor to soil pH variation after natural factors such as soil parent rock, weathering time span, climate, and vegetation. The current study assessed the potential effect of cell-free supernatant (CFS) obtained from <i>Bacillus subtilis</i> EB2004S and <i>Lactobacillus helveticus</i> EL2006H cultured at three pH levels (5, 7, and 8) on potato (var Goldrush) growth enhancement in a greenhouse pot experiment. The results showed that CFSs obtained from <i>B. subtilis</i> EB2004S and <i>L. helveticus</i> EL2006H cultured at pH 5 significantly improved photosynthetic rates, stomatal conductance, root fresh weight, and whole plant fresh weight. interactive effects of pot pH and that of CFSs obtained from pH 5 influenced chlorophyll, plant height, and shoot and whole plant fresh weight. Moreover, treatment 52EB2004S~0.4% initiated early tuberization for potato grown at pH 7 and 8. Potato grown at pH 5, which received a 72EB2004S~0.4% CFS treatment, had greater whole plant fresh and dry weight than that treated with <i>L. helveticus</i> EL2006H CFS and a positive control. Taken together, the findings of this study are unique in that it probed the effect of CFS produced under differing pH conditions which revealed a new possibility to mitigate stresses in plants. |
| first_indexed | 2024-03-11T05:34:51Z |
| format | Article |
| id | doaj.art-666c37e38c2b47068c5885c0f1c3f88a |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T05:34:51Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-666c37e38c2b47068c5885c0f1c3f88a2023-11-17T16:53:47ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-04-01247662010.3390/ijms24076620Cell-Free Supernatant (CFS) from <i>Bacillus subtilis</i> EB2004S and <i>Lactobacillus helveticus</i> EL2006H Cultured at a Range of pH Levels Modulates Potato Plant Growth under Greenhouse ConditionsLevini A. Msimbira0Judith Naamala1Sowmyalakshmi Subramanian2Donald L. Smith3Department of Plant Science, McGill University, Montreal, QC H9X 3V9, CanadaDepartment of Plant Science, McGill University, Montreal, QC H9X 3V9, CanadaDepartment of Plant Science, McGill University, Montreal, QC H9X 3V9, CanadaDepartment of Plant Science, McGill University, Montreal, QC H9X 3V9, CanadaAgriculture involving industrial fertilizers is another major human made contributing factor to soil pH variation after natural factors such as soil parent rock, weathering time span, climate, and vegetation. The current study assessed the potential effect of cell-free supernatant (CFS) obtained from <i>Bacillus subtilis</i> EB2004S and <i>Lactobacillus helveticus</i> EL2006H cultured at three pH levels (5, 7, and 8) on potato (var Goldrush) growth enhancement in a greenhouse pot experiment. The results showed that CFSs obtained from <i>B. subtilis</i> EB2004S and <i>L. helveticus</i> EL2006H cultured at pH 5 significantly improved photosynthetic rates, stomatal conductance, root fresh weight, and whole plant fresh weight. interactive effects of pot pH and that of CFSs obtained from pH 5 influenced chlorophyll, plant height, and shoot and whole plant fresh weight. Moreover, treatment 52EB2004S~0.4% initiated early tuberization for potato grown at pH 7 and 8. Potato grown at pH 5, which received a 72EB2004S~0.4% CFS treatment, had greater whole plant fresh and dry weight than that treated with <i>L. helveticus</i> EL2006H CFS and a positive control. Taken together, the findings of this study are unique in that it probed the effect of CFS produced under differing pH conditions which revealed a new possibility to mitigate stresses in plants.https://www.mdpi.com/1422-0067/24/7/6620plant growth-promoting microbescell-free supernatantpH–microbe–plant interactionbiostimulantsoil acidity and alkalinity |
| spellingShingle | Levini A. Msimbira Judith Naamala Sowmyalakshmi Subramanian Donald L. Smith Cell-Free Supernatant (CFS) from <i>Bacillus subtilis</i> EB2004S and <i>Lactobacillus helveticus</i> EL2006H Cultured at a Range of pH Levels Modulates Potato Plant Growth under Greenhouse Conditions International Journal of Molecular Sciences plant growth-promoting microbes cell-free supernatant pH–microbe–plant interaction biostimulant soil acidity and alkalinity |
| title | Cell-Free Supernatant (CFS) from <i>Bacillus subtilis</i> EB2004S and <i>Lactobacillus helveticus</i> EL2006H Cultured at a Range of pH Levels Modulates Potato Plant Growth under Greenhouse Conditions |
| title_full | Cell-Free Supernatant (CFS) from <i>Bacillus subtilis</i> EB2004S and <i>Lactobacillus helveticus</i> EL2006H Cultured at a Range of pH Levels Modulates Potato Plant Growth under Greenhouse Conditions |
| title_fullStr | Cell-Free Supernatant (CFS) from <i>Bacillus subtilis</i> EB2004S and <i>Lactobacillus helveticus</i> EL2006H Cultured at a Range of pH Levels Modulates Potato Plant Growth under Greenhouse Conditions |
| title_full_unstemmed | Cell-Free Supernatant (CFS) from <i>Bacillus subtilis</i> EB2004S and <i>Lactobacillus helveticus</i> EL2006H Cultured at a Range of pH Levels Modulates Potato Plant Growth under Greenhouse Conditions |
| title_short | Cell-Free Supernatant (CFS) from <i>Bacillus subtilis</i> EB2004S and <i>Lactobacillus helveticus</i> EL2006H Cultured at a Range of pH Levels Modulates Potato Plant Growth under Greenhouse Conditions |
| title_sort | cell free supernatant cfs from i bacillus subtilis i eb2004s and i lactobacillus helveticus i el2006h cultured at a range of ph levels modulates potato plant growth under greenhouse conditions |
| topic | plant growth-promoting microbes cell-free supernatant pH–microbe–plant interaction biostimulant soil acidity and alkalinity |
| url | https://www.mdpi.com/1422-0067/24/7/6620 |
| work_keys_str_mv | AT leviniamsimbira cellfreesupernatantcfsfromibacillussubtilisieb2004sandilactobacillushelveticusiel2006hculturedatarangeofphlevelsmodulatespotatoplantgrowthundergreenhouseconditions AT judithnaamala cellfreesupernatantcfsfromibacillussubtilisieb2004sandilactobacillushelveticusiel2006hculturedatarangeofphlevelsmodulatespotatoplantgrowthundergreenhouseconditions AT sowmyalakshmisubramanian cellfreesupernatantcfsfromibacillussubtilisieb2004sandilactobacillushelveticusiel2006hculturedatarangeofphlevelsmodulatespotatoplantgrowthundergreenhouseconditions AT donaldlsmith cellfreesupernatantcfsfromibacillussubtilisieb2004sandilactobacillushelveticusiel2006hculturedatarangeofphlevelsmodulatespotatoplantgrowthundergreenhouseconditions |