Sensitive impedimetric biosensor for direct detection of diazinon based on lipases
Two novel impedimetric biosensors for highly sensitive and rapid quantitative detection of diazinon in an aqueous medium were developed using two types of lipase, from Candida Rugosa (microbial source) (CRL) and from porcine pancreas (animal source) (PPL) immobilized onto a functionalized gold elect...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2014-07-01
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| Series: | Frontiers in Chemistry |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fchem.2014.00044/full |
| _version_ | 1818008356599627776 |
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| author | Nicole J Jaffrezic-Renault Nedjla eZehani Sergei V Dzyadevych Rochdi eKherrat |
| author_facet | Nicole J Jaffrezic-Renault Nedjla eZehani Sergei V Dzyadevych Rochdi eKherrat |
| author_sort | Nicole J Jaffrezic-Renault |
| collection | DOAJ |
| description | Two novel impedimetric biosensors for highly sensitive and rapid quantitative detection of diazinon in an aqueous medium were developed using two types of lipase, from Candida Rugosa (microbial source) (CRL) and from porcine pancreas (animal source) (PPL) immobilized onto a functionalized gold electrode. The lipase is characterized to specifically catalyze the hydrolysis of ester functions leading to the transformation of diazinon into diethyl phosphorothioic acid (DETP) and 2-isopropyl-4-methyl-6-hydroxypyrimidine (IMHP). The developed biosensors both presented a large wide range of linearity up to 50µM with a detection limit of 10 nM for the CRL biosensor and 0.1 µM for the PPL biosensor. A comparative study was carried out between the two biosensors and results showed higher sensitivity for the CRL sensor. Moreover, it presented good accuracy and reproducibility, and had very good storage and multiple use stability for 25 days when stored at 4°C. |
| first_indexed | 2024-04-14T05:28:07Z |
| format | Article |
| id | doaj.art-666c4ec3a50c4ee38880ae48fb370a89 |
| institution | Directory Open Access Journal |
| issn | 2296-2646 |
| language | English |
| last_indexed | 2024-04-14T05:28:07Z |
| publishDate | 2014-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemistry |
| spelling | doaj.art-666c4ec3a50c4ee38880ae48fb370a892022-12-22T02:09:55ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462014-07-01210.3389/fchem.2014.0004497665Sensitive impedimetric biosensor for direct detection of diazinon based on lipasesNicole J Jaffrezic-Renault0Nedjla eZehani1Sergei V Dzyadevych2Rochdi eKherrat3Claude Bernard University Lyon 1Claude Bernard University Lyon 1National Academy of Sciences of UkraineUniversity of AnnabaTwo novel impedimetric biosensors for highly sensitive and rapid quantitative detection of diazinon in an aqueous medium were developed using two types of lipase, from Candida Rugosa (microbial source) (CRL) and from porcine pancreas (animal source) (PPL) immobilized onto a functionalized gold electrode. The lipase is characterized to specifically catalyze the hydrolysis of ester functions leading to the transformation of diazinon into diethyl phosphorothioic acid (DETP) and 2-isopropyl-4-methyl-6-hydroxypyrimidine (IMHP). The developed biosensors both presented a large wide range of linearity up to 50µM with a detection limit of 10 nM for the CRL biosensor and 0.1 µM for the PPL biosensor. A comparative study was carried out between the two biosensors and results showed higher sensitivity for the CRL sensor. Moreover, it presented good accuracy and reproducibility, and had very good storage and multiple use stability for 25 days when stored at 4°C.http://journal.frontiersin.org/Journal/10.3389/fchem.2014.00044/fullDiazinonLipasebiosensororganophosphate pesticidesElectrochemical impedance spectroscopy |
| spellingShingle | Nicole J Jaffrezic-Renault Nedjla eZehani Sergei V Dzyadevych Rochdi eKherrat Sensitive impedimetric biosensor for direct detection of diazinon based on lipases Frontiers in Chemistry Diazinon Lipase biosensor organophosphate pesticides Electrochemical impedance spectroscopy |
| title | Sensitive impedimetric biosensor for direct detection of diazinon based on lipases |
| title_full | Sensitive impedimetric biosensor for direct detection of diazinon based on lipases |
| title_fullStr | Sensitive impedimetric biosensor for direct detection of diazinon based on lipases |
| title_full_unstemmed | Sensitive impedimetric biosensor for direct detection of diazinon based on lipases |
| title_short | Sensitive impedimetric biosensor for direct detection of diazinon based on lipases |
| title_sort | sensitive impedimetric biosensor for direct detection of diazinon based on lipases |
| topic | Diazinon Lipase biosensor organophosphate pesticides Electrochemical impedance spectroscopy |
| url | http://journal.frontiersin.org/Journal/10.3389/fchem.2014.00044/full |
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