Microshell Arrays Enhanced Sensitivity in Detection of Specific Antibody for Reduced Graphene Oxide Optical Sensor
Protein-protein interactions play an important role in the investigation of biomolecules. In this paper, we reported on the use of a reduced graphene oxide microshell (RGOM)-based optical biosensor for the determination of goat anti-rabbit IgG. The biosensor was prepared through a self-assembly of m...
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MDPI AG
2017-01-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/17/2/221 |
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| author | Wen-Shuai Jiang Wei Xin Shao-Nan Chen Cun-Bo Li Xiao-Guang Gao Lei-Ting Pan Zhi-Bo Liu Jian-Guo Tian |
| author_facet | Wen-Shuai Jiang Wei Xin Shao-Nan Chen Cun-Bo Li Xiao-Guang Gao Lei-Ting Pan Zhi-Bo Liu Jian-Guo Tian |
| author_sort | Wen-Shuai Jiang |
| collection | DOAJ |
| description | Protein-protein interactions play an important role in the investigation of biomolecules. In this paper, we reported on the use of a reduced graphene oxide microshell (RGOM)-based optical biosensor for the determination of goat anti-rabbit IgG. The biosensor was prepared through a self-assembly of monolayers of monodisperse polystyrene microspheres, combined with a high-temperature reduction, in order to decorate the RGOM with rabbit IgG. The periodic microshells allowed a simpler functionalization and modification of RGOM with bioreceptor units, than reduced graphene oxide (RGO). With additional antibody-antigen binding, the RGOM-based biosensor achieved better real-time and label-free detection. The RGOM-based biosensor presented a more satisfactory response to goat anti-rabbit IgG than the RGO-based biosensor. This method is promising for immobilizing biomolecules on graphene surfaces and for the fabrication of biosensors with enhanced sensitivity. |
| first_indexed | 2024-04-11T10:58:42Z |
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| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T10:58:42Z |
| publishDate | 2017-01-01 |
| publisher | MDPI AG |
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| spelling | doaj.art-667007576d8c40d1abc4dc6d5eb992472022-12-22T04:28:41ZengMDPI AGSensors1424-82202017-01-0117222110.3390/s17020221s17020221Microshell Arrays Enhanced Sensitivity in Detection of Specific Antibody for Reduced Graphene Oxide Optical SensorWen-Shuai Jiang0Wei Xin1Shao-Nan Chen2Cun-Bo Li3Xiao-Guang Gao4Lei-Ting Pan5Zhi-Bo Liu6Jian-Guo Tian7The Key Laboratory of Weak Light Nonlinear Photonics, Ministry of Education, Teda Applied Physics School and School of Physics, Nankai University, Tianjin 300071, ChinaThe Key Laboratory of Weak Light Nonlinear Photonics, Ministry of Education, Teda Applied Physics School and School of Physics, Nankai University, Tianjin 300071, ChinaThe Key Laboratory of Weak Light Nonlinear Photonics, Ministry of Education, Teda Applied Physics School and School of Physics, Nankai University, Tianjin 300071, ChinaThe Key Laboratory of Weak Light Nonlinear Photonics, Ministry of Education, Teda Applied Physics School and School of Physics, Nankai University, Tianjin 300071, ChinaThe Key Laboratory of Weak Light Nonlinear Photonics, Ministry of Education, Teda Applied Physics School and School of Physics, Nankai University, Tianjin 300071, ChinaThe Key Laboratory of Weak Light Nonlinear Photonics, Ministry of Education, Teda Applied Physics School and School of Physics, Nankai University, Tianjin 300071, ChinaThe Key Laboratory of Weak Light Nonlinear Photonics, Ministry of Education, Teda Applied Physics School and School of Physics, Nankai University, Tianjin 300071, ChinaThe Key Laboratory of Weak Light Nonlinear Photonics, Ministry of Education, Teda Applied Physics School and School of Physics, Nankai University, Tianjin 300071, ChinaProtein-protein interactions play an important role in the investigation of biomolecules. In this paper, we reported on the use of a reduced graphene oxide microshell (RGOM)-based optical biosensor for the determination of goat anti-rabbit IgG. The biosensor was prepared through a self-assembly of monolayers of monodisperse polystyrene microspheres, combined with a high-temperature reduction, in order to decorate the RGOM with rabbit IgG. The periodic microshells allowed a simpler functionalization and modification of RGOM with bioreceptor units, than reduced graphene oxide (RGO). With additional antibody-antigen binding, the RGOM-based biosensor achieved better real-time and label-free detection. The RGOM-based biosensor presented a more satisfactory response to goat anti-rabbit IgG than the RGO-based biosensor. This method is promising for immobilizing biomolecules on graphene surfaces and for the fabrication of biosensors with enhanced sensitivity.http://www.mdpi.com/1424-8220/17/2/221reduced graphene oxidemicroshellsoptical sensorgoat anti-rabbit IgGreal-timelabel-free |
| spellingShingle | Wen-Shuai Jiang Wei Xin Shao-Nan Chen Cun-Bo Li Xiao-Guang Gao Lei-Ting Pan Zhi-Bo Liu Jian-Guo Tian Microshell Arrays Enhanced Sensitivity in Detection of Specific Antibody for Reduced Graphene Oxide Optical Sensor Sensors reduced graphene oxide microshells optical sensor goat anti-rabbit IgG real-time label-free |
| title | Microshell Arrays Enhanced Sensitivity in Detection of Specific Antibody for Reduced Graphene Oxide Optical Sensor |
| title_full | Microshell Arrays Enhanced Sensitivity in Detection of Specific Antibody for Reduced Graphene Oxide Optical Sensor |
| title_fullStr | Microshell Arrays Enhanced Sensitivity in Detection of Specific Antibody for Reduced Graphene Oxide Optical Sensor |
| title_full_unstemmed | Microshell Arrays Enhanced Sensitivity in Detection of Specific Antibody for Reduced Graphene Oxide Optical Sensor |
| title_short | Microshell Arrays Enhanced Sensitivity in Detection of Specific Antibody for Reduced Graphene Oxide Optical Sensor |
| title_sort | microshell arrays enhanced sensitivity in detection of specific antibody for reduced graphene oxide optical sensor |
| topic | reduced graphene oxide microshells optical sensor goat anti-rabbit IgG real-time label-free |
| url | http://www.mdpi.com/1424-8220/17/2/221 |
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