Discovery of Unexpected Sphingolipids in Almonds and Pistachios with an Innovative Use of Triple Quadrupole Tandem Mass Spectrometry
The densely packed storage of valuable nutrients (carbohydrates, lipids, proteins, micronutrients) in the endosperm of nuts and seeds makes the study of their complex composition a topic of great importance. Ceramides in the total lipid extract of some ground almonds and pistachios were searched wit...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-01-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/9/2/110 |
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| author | Federico Maria Rubino Michele Dei Cas Monica Bignotto Riccardo Ghidoni Marcello Iriti Rita Paroni |
| author_facet | Federico Maria Rubino Michele Dei Cas Monica Bignotto Riccardo Ghidoni Marcello Iriti Rita Paroni |
| author_sort | Federico Maria Rubino |
| collection | DOAJ |
| description | The densely packed storage of valuable nutrients (carbohydrates, lipids, proteins, micronutrients) in the endosperm of nuts and seeds makes the study of their complex composition a topic of great importance. Ceramides in the total lipid extract of some ground almonds and pistachios were searched with a systematic innovative discovery precursor ion scan in a triple quadrupole tandem mass spectrometry, where iso-energetic collision activated dissociation was performed. Five descriptors were used to search components with different C18 long chain bases containing different structural motifs (d18:0, d18:1, d18:2, t18:0, t18:1). The presence of hexoside unit was screened with a specific neutral loss experiment under iso-energetic collision activated dissociation conditions. The discovery scans highlighted the presence of two specific hexosyl-ceramides with a modified sphingosine component (d18:2) and C16:0 or C16:0 hydroxy-fatty acids. The hexosyl-ceramide with the non-hydroxylated fatty acid seemed specific of pistachios and was undetected in almonds. The fast and comprehensive mass spectrometric method used here can be useful to screen lipid extracts of several more seeds of nutraceutical interest, searching for unusual and/or specific sphingosides with chemically decorated long chain bases. |
| first_indexed | 2024-12-10T05:06:13Z |
| format | Article |
| id | doaj.art-6672095096784010b9f7af34eb0f9837 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-12-10T05:06:13Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-6672095096784010b9f7af34eb0f98372022-12-22T02:01:14ZengMDPI AGFoods2304-81582020-01-019211010.3390/foods9020110foods9020110Discovery of Unexpected Sphingolipids in Almonds and Pistachios with an Innovative Use of Triple Quadrupole Tandem Mass SpectrometryFederico Maria Rubino0Michele Dei Cas1Monica Bignotto2Riccardo Ghidoni3Marcello Iriti4Rita Paroni5Dipartimento di Scienze della Salute, Universita’ degli Studi di Milano, I-20142 Milano, ItalyDipartimento di Scienze della Salute, Universita’ degli Studi di Milano, I-20142 Milano, ItalyDipartimento di Scienze della Salute, Universita’ degli Studi di Milano, I-20142 Milano, ItalyDipartimento di Scienze della Salute, Universita’ degli Studi di Milano, I-20142 Milano, ItalyDipartimento di Scienze Agrarie e Ambientali—Produzione, Territorio, Agroenergia, Universita’ degli Studi di Milano, I-20133 Milano, ItalyDipartimento di Scienze della Salute, Universita’ degli Studi di Milano, I-20142 Milano, ItalyThe densely packed storage of valuable nutrients (carbohydrates, lipids, proteins, micronutrients) in the endosperm of nuts and seeds makes the study of their complex composition a topic of great importance. Ceramides in the total lipid extract of some ground almonds and pistachios were searched with a systematic innovative discovery precursor ion scan in a triple quadrupole tandem mass spectrometry, where iso-energetic collision activated dissociation was performed. Five descriptors were used to search components with different C18 long chain bases containing different structural motifs (d18:0, d18:1, d18:2, t18:0, t18:1). The presence of hexoside unit was screened with a specific neutral loss experiment under iso-energetic collision activated dissociation conditions. The discovery scans highlighted the presence of two specific hexosyl-ceramides with a modified sphingosine component (d18:2) and C16:0 or C16:0 hydroxy-fatty acids. The hexosyl-ceramide with the non-hydroxylated fatty acid seemed specific of pistachios and was undetected in almonds. The fast and comprehensive mass spectrometric method used here can be useful to screen lipid extracts of several more seeds of nutraceutical interest, searching for unusual and/or specific sphingosides with chemically decorated long chain bases.https://www.mdpi.com/2304-8158/9/2/110ceramideslipidsfunctional foodmass spectrometrynutraceuticalstraditional food |
| spellingShingle | Federico Maria Rubino Michele Dei Cas Monica Bignotto Riccardo Ghidoni Marcello Iriti Rita Paroni Discovery of Unexpected Sphingolipids in Almonds and Pistachios with an Innovative Use of Triple Quadrupole Tandem Mass Spectrometry Foods ceramides lipids functional food mass spectrometry nutraceuticals traditional food |
| title | Discovery of Unexpected Sphingolipids in Almonds and Pistachios with an Innovative Use of Triple Quadrupole Tandem Mass Spectrometry |
| title_full | Discovery of Unexpected Sphingolipids in Almonds and Pistachios with an Innovative Use of Triple Quadrupole Tandem Mass Spectrometry |
| title_fullStr | Discovery of Unexpected Sphingolipids in Almonds and Pistachios with an Innovative Use of Triple Quadrupole Tandem Mass Spectrometry |
| title_full_unstemmed | Discovery of Unexpected Sphingolipids in Almonds and Pistachios with an Innovative Use of Triple Quadrupole Tandem Mass Spectrometry |
| title_short | Discovery of Unexpected Sphingolipids in Almonds and Pistachios with an Innovative Use of Triple Quadrupole Tandem Mass Spectrometry |
| title_sort | discovery of unexpected sphingolipids in almonds and pistachios with an innovative use of triple quadrupole tandem mass spectrometry |
| topic | ceramides lipids functional food mass spectrometry nutraceuticals traditional food |
| url | https://www.mdpi.com/2304-8158/9/2/110 |
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