Establishment of multiplex PCR for detection of genes related to Quinolone resistance
Background/aims: It has been shown that quinolone resistance arises due to mutations in the quinolone resistance-determining regions of the drug targets. This study aimed to optimise a multiplex PCR assay to track plasmid-mediated low-level quinolone resistance profiles. Subjects and methods: A...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Vietnam Ministry of Science and Technology
2018-06-01
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| Series: | Vietnam Journal of Science, Technology and Engineering |
| Subjects: | |
| Online Access: | https://vietnamscience.vjst.vn/index.php/vjste/article/view/243 |
| _version_ | 1797937755370553344 |
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| author | Ngo Tat Trung Dao Thanh Quyen Phan Quoc Hoan Thirumalaisamy P.P. Velavan Le Huu Song |
| author_facet | Ngo Tat Trung Dao Thanh Quyen Phan Quoc Hoan Thirumalaisamy P.P. Velavan Le Huu Song |
| author_sort | Ngo Tat Trung |
| collection | DOAJ |
| description |
Background/aims: It has been shown that quinolone resistance arises due to mutations in the quinolone resistance-determining regions of the drug targets. This study aimed to optimise a multiplex PCR assay to track plasmid-mediated low-level quinolone resistance profiles.
Subjects and methods: A multiplex PCR-based-method in which the primers were already established by our team. About 44 samples were collected from 44 patients who enrolled in this study by using surgical site infection (SSI).
Results: By targeting the conserved domains of qnrA, qnrB, qnrS and the qnrVC gene families, the primer number was reduced significantly to only four pairs in one multiplex PCR. Using multiplex PCR, 3/44 SSI samples were found to be carrying fluoroquinoloneresistance genes (qnrA, qnrB, qnrS, qnrVC).
Conclusion: A multiplex PCR for detecting pathogens as well as identifying quinolone resistance genes all in one reaction was successfully established.
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| first_indexed | 2024-04-10T18:49:15Z |
| format | Article |
| id | doaj.art-667711d8bc0d44d2a2c78e6c9a542702 |
| institution | Directory Open Access Journal |
| issn | 2525-2461 2615-9937 |
| language | English |
| last_indexed | 2024-04-10T18:49:15Z |
| publishDate | 2018-06-01 |
| publisher | Vietnam Ministry of Science and Technology |
| record_format | Article |
| series | Vietnam Journal of Science, Technology and Engineering |
| spelling | doaj.art-667711d8bc0d44d2a2c78e6c9a5427022023-02-01T08:20:36ZengVietnam Ministry of Science and TechnologyVietnam Journal of Science, Technology and Engineering2525-24612615-99372018-06-0160210.31276/VJSTE.60(2).51Establishment of multiplex PCR for detection of genes related to Quinolone resistanceNgo Tat Trung0Dao Thanh Quyen1Phan Quoc Hoan2Thirumalaisamy P.P. Velavan3Le Huu Song4Dept. of Molecular Biology, 108 Military Central Hospital, Hanoi, VietnamDept. of Molecular Biology, 108 Military Central Hospital, Hanoi, VietnamDept. of Molecular Biology, 108 Military Central Hospital, Hanoi, VietnamInstitute of Tropical Medicine, Tübingen University, GermanyDept. of Molecular Biology, 108 Military Central Hospital, Hanoi, Vietnam; Faculty of Infectious and Tropical Diseases, 108 Military Central Hospital, Hanoi, Vietnam Background/aims: It has been shown that quinolone resistance arises due to mutations in the quinolone resistance-determining regions of the drug targets. This study aimed to optimise a multiplex PCR assay to track plasmid-mediated low-level quinolone resistance profiles. Subjects and methods: A multiplex PCR-based-method in which the primers were already established by our team. About 44 samples were collected from 44 patients who enrolled in this study by using surgical site infection (SSI). Results: By targeting the conserved domains of qnrA, qnrB, qnrS and the qnrVC gene families, the primer number was reduced significantly to only four pairs in one multiplex PCR. Using multiplex PCR, 3/44 SSI samples were found to be carrying fluoroquinoloneresistance genes (qnrA, qnrB, qnrS, qnrVC). Conclusion: A multiplex PCR for detecting pathogens as well as identifying quinolone resistance genes all in one reaction was successfully established. https://vietnamscience.vjst.vn/index.php/vjste/article/view/243plasmid-mediated quinolone resistance genesplasmidsSSIqnr |
| spellingShingle | Ngo Tat Trung Dao Thanh Quyen Phan Quoc Hoan Thirumalaisamy P.P. Velavan Le Huu Song Establishment of multiplex PCR for detection of genes related to Quinolone resistance Vietnam Journal of Science, Technology and Engineering plasmid-mediated quinolone resistance genes plasmids SSI qnr |
| title | Establishment of multiplex PCR for detection of genes related to Quinolone resistance |
| title_full | Establishment of multiplex PCR for detection of genes related to Quinolone resistance |
| title_fullStr | Establishment of multiplex PCR for detection of genes related to Quinolone resistance |
| title_full_unstemmed | Establishment of multiplex PCR for detection of genes related to Quinolone resistance |
| title_short | Establishment of multiplex PCR for detection of genes related to Quinolone resistance |
| title_sort | establishment of multiplex pcr for detection of genes related to quinolone resistance |
| topic | plasmid-mediated quinolone resistance genes plasmids SSI qnr |
| url | https://vietnamscience.vjst.vn/index.php/vjste/article/view/243 |
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