An aerotaxis receptor influences invasion of Agrobacterium tumefaciens into its host
Agrobacterium tumefaciens is a soil-borne pathogenic bacterium that causes crown gall disease in many plants. Chemotaxis offers A. tumefaciens the ability to find its host and establish infection. Being an aerobic bacterium, A. tumefaciens possesses one chemotaxis system with multiple potential chem...
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PeerJ Inc.
2024-02-01
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| author | Zhiwei Huang Junnan Zou Minliang Guo Guoliang Zhang Jun Gao Hongliang Zhao Feiyu Yan Yuan Niu Guang-Long Wang |
| author_facet | Zhiwei Huang Junnan Zou Minliang Guo Guoliang Zhang Jun Gao Hongliang Zhao Feiyu Yan Yuan Niu Guang-Long Wang |
| author_sort | Zhiwei Huang |
| collection | DOAJ |
| description | Agrobacterium tumefaciens is a soil-borne pathogenic bacterium that causes crown gall disease in many plants. Chemotaxis offers A. tumefaciens the ability to find its host and establish infection. Being an aerobic bacterium, A. tumefaciens possesses one chemotaxis system with multiple potential chemoreceptors. Chemoreceptors play an important role in perceiving and responding to environmental signals. However, the studies of chemoreceptors in A. tumefaciens remain relatively restricted. Here, we characterized a cytoplasmic chemoreceptor of A. tumefaciens C58 that contains an N-terminal globin domain. The chemoreceptor was designated as Atu1027. The deletion of Atu1027 not only eliminated the aerotactic response of A. tumefaciens to atmospheric air but also resulted in a weakened chemotactic response to multiple carbon sources. Subsequent site-directed mutagenesis and phenotypic analysis showed that the conserved residue His100 in Atu1027 is essential for the globin domain’s function in both chemotaxis and aerotaxis. Furthermore, deleting Atu1027 impaired the biofilm formation and pathogenicity of A. tumefaciens. Collectively, our findings demonstrated that Atu1027 functions as an aerotaxis receptor that affects agrobacterial chemotaxis and the invasion of A. tumefaciens into its host. |
| first_indexed | 2024-03-08T04:57:26Z |
| format | Article |
| id | doaj.art-6678a6e89e7848338b6c27e752b33c9c |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-08T04:57:26Z |
| publishDate | 2024-02-01 |
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| spelling | doaj.art-6678a6e89e7848338b6c27e752b33c9c2024-02-07T15:05:08ZengPeerJ Inc.PeerJ2167-83592024-02-0112e1689810.7717/peerj.16898An aerotaxis receptor influences invasion of Agrobacterium tumefaciens into its hostZhiwei Huang0Junnan Zou1Minliang Guo2Guoliang Zhang3Jun Gao4Hongliang Zhao5Feiyu Yan6Yuan Niu7Guang-Long Wang8Jiangsu Provincial Agricultural Green and Low Carbon Production Technology Engineering Research Center, School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an, Jiangsu Province, ChinaJiangsu Provincial Agricultural Green and Low Carbon Production Technology Engineering Research Center, School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an, Jiangsu Province, ChinaCollege of Bioscience and Biotechnology, Yangzhou University, Yangzhou City, Jiangsu Province, ChinaJiangsu Provincial Agricultural Green and Low Carbon Production Technology Engineering Research Center, School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an, Jiangsu Province, ChinaJiangsu Provincial Agricultural Green and Low Carbon Production Technology Engineering Research Center, School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an, Jiangsu Province, ChinaJiangsu Provincial Agricultural Green and Low Carbon Production Technology Engineering Research Center, School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an, Jiangsu Province, ChinaJiangsu Provincial Agricultural Green and Low Carbon Production Technology Engineering Research Center, School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an, Jiangsu Province, ChinaJiangsu Provincial Agricultural Green and Low Carbon Production Technology Engineering Research Center, School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an, Jiangsu Province, ChinaJiangsu Provincial Agricultural Green and Low Carbon Production Technology Engineering Research Center, School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an, Jiangsu Province, ChinaAgrobacterium tumefaciens is a soil-borne pathogenic bacterium that causes crown gall disease in many plants. Chemotaxis offers A. tumefaciens the ability to find its host and establish infection. Being an aerobic bacterium, A. tumefaciens possesses one chemotaxis system with multiple potential chemoreceptors. Chemoreceptors play an important role in perceiving and responding to environmental signals. However, the studies of chemoreceptors in A. tumefaciens remain relatively restricted. Here, we characterized a cytoplasmic chemoreceptor of A. tumefaciens C58 that contains an N-terminal globin domain. The chemoreceptor was designated as Atu1027. The deletion of Atu1027 not only eliminated the aerotactic response of A. tumefaciens to atmospheric air but also resulted in a weakened chemotactic response to multiple carbon sources. Subsequent site-directed mutagenesis and phenotypic analysis showed that the conserved residue His100 in Atu1027 is essential for the globin domain’s function in both chemotaxis and aerotaxis. Furthermore, deleting Atu1027 impaired the biofilm formation and pathogenicity of A. tumefaciens. Collectively, our findings demonstrated that Atu1027 functions as an aerotaxis receptor that affects agrobacterial chemotaxis and the invasion of A. tumefaciens into its host.https://peerj.com/articles/16898.pdfAgrobacterium tumefaciensChemotaxisAerotaxisChemoreceptorBiofilm formationPathogenicity |
| spellingShingle | Zhiwei Huang Junnan Zou Minliang Guo Guoliang Zhang Jun Gao Hongliang Zhao Feiyu Yan Yuan Niu Guang-Long Wang An aerotaxis receptor influences invasion of Agrobacterium tumefaciens into its host PeerJ Agrobacterium tumefaciens Chemotaxis Aerotaxis Chemoreceptor Biofilm formation Pathogenicity |
| title | An aerotaxis receptor influences invasion of Agrobacterium tumefaciens into its host |
| title_full | An aerotaxis receptor influences invasion of Agrobacterium tumefaciens into its host |
| title_fullStr | An aerotaxis receptor influences invasion of Agrobacterium tumefaciens into its host |
| title_full_unstemmed | An aerotaxis receptor influences invasion of Agrobacterium tumefaciens into its host |
| title_short | An aerotaxis receptor influences invasion of Agrobacterium tumefaciens into its host |
| title_sort | aerotaxis receptor influences invasion of agrobacterium tumefaciens into its host |
| topic | Agrobacterium tumefaciens Chemotaxis Aerotaxis Chemoreceptor Biofilm formation Pathogenicity |
| url | https://peerj.com/articles/16898.pdf |
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