The Rh blood group system and its role in alloimmunization rate among sickle cell disease and sickle thalassemia patients in Iran
Abstract Introduction The alloimmunization following blood transfusion can be life‐threatening. The Rh alloantibodies are one of the most common causes contributing to alloimmunization. This study aimed to evaluate the rate and causes of alloimmunization and to determine the Rh phenotypes and genoty...
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| Format: | Article |
| Language: | English |
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Wiley
2021-03-01
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| Series: | Molecular Genetics & Genomic Medicine |
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| Online Access: | https://doi.org/10.1002/mgg3.1614 |
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| author | Mohammad Ali Jalali Far Arezoo Oodi Naser Amirizadeh Mahshid Mohammadipour Bijan Keikhaei Dehdezi |
| author_facet | Mohammad Ali Jalali Far Arezoo Oodi Naser Amirizadeh Mahshid Mohammadipour Bijan Keikhaei Dehdezi |
| author_sort | Mohammad Ali Jalali Far |
| collection | DOAJ |
| description | Abstract Introduction The alloimmunization following blood transfusion can be life‐threatening. The Rh alloantibodies are one of the most common causes contributing to alloimmunization. This study aimed to evaluate the rate and causes of alloimmunization and to determine the Rh phenotypes and genotypes among sickle cell disease (SCD) and sickle thalassemia (Sβ). Materials and Methods Our study included 104 SCD and Sβ patients referring to Baghaei 2 Hospital of Ahvaz in 2019 using a non‐random simple sampling method. The blood samples were collected for Rh phenotypes, alloantibody screening and identification, and molecular tests. The SSP‐PCR and RFLP methods with the Pst 1 enzyme were used. Results The alloimmunization rate was 9.6% and 13.2% based on immunohematological tests and medical records, respectively. The main alloantibodies (90%) were anti‐Rh, and 40% of the patients had multiple alloantibodies. A significant correlation was found between gender and alloimmunization. The phenotypes of DCce (37.5%), DCcEe (24%), Dce (20.2%), and dce (5.8%) and genotypes of R1r (25%), R1R2 (20.2%), R1R1 (18.3%), and R1R0 (10.6%) were the most prevalent. The R1R2 was a frequent genotype in Sβ. Conclusion R0r′ and R1R0 genotypes were limited to our population in Iran. Due to the differences in RH genotypes between our population and others, the blood transfusion from other ethnicities increased our total alloimmunization rate. |
| first_indexed | 2024-12-16T11:23:48Z |
| format | Article |
| id | doaj.art-667b5173a9514fe3983d90fd4fb50003 |
| institution | Directory Open Access Journal |
| issn | 2324-9269 |
| language | English |
| last_indexed | 2024-12-16T11:23:48Z |
| publishDate | 2021-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Molecular Genetics & Genomic Medicine |
| spelling | doaj.art-667b5173a9514fe3983d90fd4fb500032022-12-21T22:33:24ZengWileyMolecular Genetics & Genomic Medicine2324-92692021-03-0193n/an/a10.1002/mgg3.1614The Rh blood group system and its role in alloimmunization rate among sickle cell disease and sickle thalassemia patients in IranMohammad Ali Jalali Far0Arezoo Oodi1Naser Amirizadeh2Mahshid Mohammadipour3Bijan Keikhaei Dehdezi4Blood Transfusion Research Center High Institute for Research and Education in Transfusion Medicine Tehran IranBlood Transfusion Research Center High Institute for Research and Education in Transfusion Medicine Tehran IranBlood Transfusion Research Center High Institute for Research and Education in Transfusion Medicine Tehran IranBlood Transfusion Research Center High Institute for Research and Education in Transfusion Medicine Tehran IranThalassemia & Hemoglobinopathy Research Center, Research Institute of Health Ahvaz Jundishapur University of Medical Sciences Ahvaz IranAbstract Introduction The alloimmunization following blood transfusion can be life‐threatening. The Rh alloantibodies are one of the most common causes contributing to alloimmunization. This study aimed to evaluate the rate and causes of alloimmunization and to determine the Rh phenotypes and genotypes among sickle cell disease (SCD) and sickle thalassemia (Sβ). Materials and Methods Our study included 104 SCD and Sβ patients referring to Baghaei 2 Hospital of Ahvaz in 2019 using a non‐random simple sampling method. The blood samples were collected for Rh phenotypes, alloantibody screening and identification, and molecular tests. The SSP‐PCR and RFLP methods with the Pst 1 enzyme were used. Results The alloimmunization rate was 9.6% and 13.2% based on immunohematological tests and medical records, respectively. The main alloantibodies (90%) were anti‐Rh, and 40% of the patients had multiple alloantibodies. A significant correlation was found between gender and alloimmunization. The phenotypes of DCce (37.5%), DCcEe (24%), Dce (20.2%), and dce (5.8%) and genotypes of R1r (25%), R1R2 (20.2%), R1R1 (18.3%), and R1R0 (10.6%) were the most prevalent. The R1R2 was a frequent genotype in Sβ. Conclusion R0r′ and R1R0 genotypes were limited to our population in Iran. Due to the differences in RH genotypes between our population and others, the blood transfusion from other ethnicities increased our total alloimmunization rate.https://doi.org/10.1002/mgg3.1614alloimmunizationRH genotypeRh phenotypesickle cell diseasesickle thalassemia |
| spellingShingle | Mohammad Ali Jalali Far Arezoo Oodi Naser Amirizadeh Mahshid Mohammadipour Bijan Keikhaei Dehdezi The Rh blood group system and its role in alloimmunization rate among sickle cell disease and sickle thalassemia patients in Iran Molecular Genetics & Genomic Medicine alloimmunization RH genotype Rh phenotype sickle cell disease sickle thalassemia |
| title | The Rh blood group system and its role in alloimmunization rate among sickle cell disease and sickle thalassemia patients in Iran |
| title_full | The Rh blood group system and its role in alloimmunization rate among sickle cell disease and sickle thalassemia patients in Iran |
| title_fullStr | The Rh blood group system and its role in alloimmunization rate among sickle cell disease and sickle thalassemia patients in Iran |
| title_full_unstemmed | The Rh blood group system and its role in alloimmunization rate among sickle cell disease and sickle thalassemia patients in Iran |
| title_short | The Rh blood group system and its role in alloimmunization rate among sickle cell disease and sickle thalassemia patients in Iran |
| title_sort | rh blood group system and its role in alloimmunization rate among sickle cell disease and sickle thalassemia patients in iran |
| topic | alloimmunization RH genotype Rh phenotype sickle cell disease sickle thalassemia |
| url | https://doi.org/10.1002/mgg3.1614 |
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