The anti-lipopolysaccharide effect of glycine via fibroblastic cell culture from the embryonic calipash tissue of Chinese soft-shelled turtle Pelodiscus sinensis
As a representative kind of soft-shelled turtles, Pelodiscus sinensis has soft apron termed calipash around its pliable carapace as the most distinctive trait, which is functionally important in turtle defense. It is of great significance to explore the physiological function of stress resistance an...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-06-01
|
| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S235251342300114X |
| _version_ | 1797801773114589184 |
|---|---|
| author | Beining Zhou Wei Song Leyan Zhu Zilian Deng Guoying Qian Caiyan Li |
| author_facet | Beining Zhou Wei Song Leyan Zhu Zilian Deng Guoying Qian Caiyan Li |
| author_sort | Beining Zhou |
| collection | DOAJ |
| description | As a representative kind of soft-shelled turtles, Pelodiscus sinensis has soft apron termed calipash around its pliable carapace as the most distinctive trait, which is functionally important in turtle defense. It is of great significance to explore the physiological function of stress resistance and the possible regulation of anti-stress nutrition for the survival of aquaculture. To address this issue, a fibroblast cell line from the embryonic calipash tissue of P. sinensis (PSCF) was established from the perspective of ethics, repeatability, and convenience. Effect of glycine, the main amino acid component in calipash extracellular matrix, on the proliferation of PSCF treated with 160 μg/mL lipopolysaccharide (LPS) and the underlying mechanisms were also studied. The results showed that adding 100 μg/mL glycine significantly improved the fibroblast survival, increased the mRNA levels of collagen biosynthesis genes, prolyl hydroxylase and collagen degradation genes, and inhibited reactive oxygen species (ROS) production. It suggested that glycine shows positive effects on the cell proliferation of PSCF, and could regulate the oxidative stress and collagen metabolism. To our knowledge, this report is the first to describe cell cultures established at specific calipash tissue-forming stages during the embryonic development in P. sinensis and to reveal the anti-stress effect of glycine in turtles. The results will contribute to the application of functional calipash tissue, or its manipulation by nutrition additives, such as glycine, to prevent or ameliorate stress in turtle industry. |
| first_indexed | 2024-03-13T04:55:34Z |
| format | Article |
| id | doaj.art-66819eb5da284664b03e31da6cfaaf3a |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-03-13T04:55:34Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-66819eb5da284664b03e31da6cfaaf3a2023-06-18T05:02:24ZengElsevierAquaculture Reports2352-51342023-06-0130101575The anti-lipopolysaccharide effect of glycine via fibroblastic cell culture from the embryonic calipash tissue of Chinese soft-shelled turtle Pelodiscus sinensisBeining Zhou0Wei Song1Leyan Zhu2Zilian Deng3Guoying Qian4Caiyan Li5College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, Zhejiang, ChinaCollege of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, Zhejiang, ChinaCollege of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, Zhejiang, ChinaCollege of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, Zhejiang, ChinaCollege of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, Zhejiang, ChinaCorresponding author.; College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, Zhejiang, ChinaAs a representative kind of soft-shelled turtles, Pelodiscus sinensis has soft apron termed calipash around its pliable carapace as the most distinctive trait, which is functionally important in turtle defense. It is of great significance to explore the physiological function of stress resistance and the possible regulation of anti-stress nutrition for the survival of aquaculture. To address this issue, a fibroblast cell line from the embryonic calipash tissue of P. sinensis (PSCF) was established from the perspective of ethics, repeatability, and convenience. Effect of glycine, the main amino acid component in calipash extracellular matrix, on the proliferation of PSCF treated with 160 μg/mL lipopolysaccharide (LPS) and the underlying mechanisms were also studied. The results showed that adding 100 μg/mL glycine significantly improved the fibroblast survival, increased the mRNA levels of collagen biosynthesis genes, prolyl hydroxylase and collagen degradation genes, and inhibited reactive oxygen species (ROS) production. It suggested that glycine shows positive effects on the cell proliferation of PSCF, and could regulate the oxidative stress and collagen metabolism. To our knowledge, this report is the first to describe cell cultures established at specific calipash tissue-forming stages during the embryonic development in P. sinensis and to reveal the anti-stress effect of glycine in turtles. The results will contribute to the application of functional calipash tissue, or its manipulation by nutrition additives, such as glycine, to prevent or ameliorate stress in turtle industry.http://www.sciencedirect.com/science/article/pii/S235251342300114XGlycineLipopolysaccharideCell cultureCollagenCalipashPelodiscus sinensis |
| spellingShingle | Beining Zhou Wei Song Leyan Zhu Zilian Deng Guoying Qian Caiyan Li The anti-lipopolysaccharide effect of glycine via fibroblastic cell culture from the embryonic calipash tissue of Chinese soft-shelled turtle Pelodiscus sinensis Aquaculture Reports Glycine Lipopolysaccharide Cell culture Collagen Calipash Pelodiscus sinensis |
| title | The anti-lipopolysaccharide effect of glycine via fibroblastic cell culture from the embryonic calipash tissue of Chinese soft-shelled turtle Pelodiscus sinensis |
| title_full | The anti-lipopolysaccharide effect of glycine via fibroblastic cell culture from the embryonic calipash tissue of Chinese soft-shelled turtle Pelodiscus sinensis |
| title_fullStr | The anti-lipopolysaccharide effect of glycine via fibroblastic cell culture from the embryonic calipash tissue of Chinese soft-shelled turtle Pelodiscus sinensis |
| title_full_unstemmed | The anti-lipopolysaccharide effect of glycine via fibroblastic cell culture from the embryonic calipash tissue of Chinese soft-shelled turtle Pelodiscus sinensis |
| title_short | The anti-lipopolysaccharide effect of glycine via fibroblastic cell culture from the embryonic calipash tissue of Chinese soft-shelled turtle Pelodiscus sinensis |
| title_sort | anti lipopolysaccharide effect of glycine via fibroblastic cell culture from the embryonic calipash tissue of chinese soft shelled turtle pelodiscus sinensis |
| topic | Glycine Lipopolysaccharide Cell culture Collagen Calipash Pelodiscus sinensis |
| url | http://www.sciencedirect.com/science/article/pii/S235251342300114X |
| work_keys_str_mv | AT beiningzhou theantilipopolysaccharideeffectofglycineviafibroblasticcellculturefromtheembryoniccalipashtissueofchinesesoftshelledturtlepelodiscussinensis AT weisong theantilipopolysaccharideeffectofglycineviafibroblasticcellculturefromtheembryoniccalipashtissueofchinesesoftshelledturtlepelodiscussinensis AT leyanzhu theantilipopolysaccharideeffectofglycineviafibroblasticcellculturefromtheembryoniccalipashtissueofchinesesoftshelledturtlepelodiscussinensis AT ziliandeng theantilipopolysaccharideeffectofglycineviafibroblasticcellculturefromtheembryoniccalipashtissueofchinesesoftshelledturtlepelodiscussinensis AT guoyingqian theantilipopolysaccharideeffectofglycineviafibroblasticcellculturefromtheembryoniccalipashtissueofchinesesoftshelledturtlepelodiscussinensis AT caiyanli theantilipopolysaccharideeffectofglycineviafibroblasticcellculturefromtheembryoniccalipashtissueofchinesesoftshelledturtlepelodiscussinensis AT beiningzhou antilipopolysaccharideeffectofglycineviafibroblasticcellculturefromtheembryoniccalipashtissueofchinesesoftshelledturtlepelodiscussinensis AT weisong antilipopolysaccharideeffectofglycineviafibroblasticcellculturefromtheembryoniccalipashtissueofchinesesoftshelledturtlepelodiscussinensis AT leyanzhu antilipopolysaccharideeffectofglycineviafibroblasticcellculturefromtheembryoniccalipashtissueofchinesesoftshelledturtlepelodiscussinensis AT ziliandeng antilipopolysaccharideeffectofglycineviafibroblasticcellculturefromtheembryoniccalipashtissueofchinesesoftshelledturtlepelodiscussinensis AT guoyingqian antilipopolysaccharideeffectofglycineviafibroblasticcellculturefromtheembryoniccalipashtissueofchinesesoftshelledturtlepelodiscussinensis AT caiyanli antilipopolysaccharideeffectofglycineviafibroblasticcellculturefromtheembryoniccalipashtissueofchinesesoftshelledturtlepelodiscussinensis |