Immobilization of Dextranase Obtained from the Marine <i>Cellulosimicrobium</i> sp. Y1 on Nanoparticles: Nano-TiO<sub>2</sub> Improving Hydrolysate Properties and Enhancing Reuse
Dextranase is widely used in sugar production, drug synthesis, material preparation, and biotechnology, among other fields. The immobilization of dextranase using nanomaterials in order to make it reusable, is a hot research topic. In this study, the immobilization of purified dextranase was perform...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-03-01
|
| Series: | Nanomaterials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-4991/13/6/1065 |
| _version_ | 1797609779184533504 |
|---|---|
| author | Yingying Xu Huanyu Wang Qianru Lin Qingzhen Miao Mingwang Liu Hao Ni Lei Zhang Mingsheng Lyu Shujun Wang |
| author_facet | Yingying Xu Huanyu Wang Qianru Lin Qingzhen Miao Mingwang Liu Hao Ni Lei Zhang Mingsheng Lyu Shujun Wang |
| author_sort | Yingying Xu |
| collection | DOAJ |
| description | Dextranase is widely used in sugar production, drug synthesis, material preparation, and biotechnology, among other fields. The immobilization of dextranase using nanomaterials in order to make it reusable, is a hot research topic. In this study, the immobilization of purified dextranase was performed using different nanomaterials. The best results were obtained when dextranase was immobilized on titanium dioxide (TiO<sub>2</sub>), and a particle size of 30 nm was achieved. The optimum immobilization conditions were pH 7.0, temperature 25 °C, time 1 h, and immobilization agent TiO<sub>2</sub>. The immobilized materials were characterized using Fourier-transform infrared spectroscopy, X-ray diffractometry, and field emission gun scanning electron microscopy. The optimum temperature and pH of the immobilized dextranase were 30 °C and 7.5, respectively. The activity of the immobilized dextranase was >50% even after 7 times of reuse, and 58% of the enzyme was active even after 7 days of storage at 25 °C, indicating the reproducibility of the immobilized enzyme. The adsorption of dextranase by TiO<sub>2</sub> nanoparticles exhibited secondary reaction kinetics. Compared with free dextranase, the hydrolysates of the immobilized dextranase were significantly different, and consisted mainly of isomaltotriose and isomaltotetraose. The highly polymerized isomaltotetraose levels could reach >78.69% of the product after 30 min of enzymatic digestion. |
| first_indexed | 2024-03-11T06:05:08Z |
| format | Article |
| id | doaj.art-6684098b8bc842e6981b4c1ec70a135b |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-11T06:05:08Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-6684098b8bc842e6981b4c1ec70a135b2023-11-17T13:01:03ZengMDPI AGNanomaterials2079-49912023-03-01136106510.3390/nano13061065Immobilization of Dextranase Obtained from the Marine <i>Cellulosimicrobium</i> sp. Y1 on Nanoparticles: Nano-TiO<sub>2</sub> Improving Hydrolysate Properties and Enhancing ReuseYingying Xu0Huanyu Wang1Qianru Lin2Qingzhen Miao3Mingwang Liu4Hao Ni5Lei Zhang6Mingsheng Lyu7Shujun Wang8Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine, Biotechnology, Jiangsu Ocean University, Lianyungang 222005, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine, Biotechnology, Jiangsu Ocean University, Lianyungang 222005, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine, Biotechnology, Jiangsu Ocean University, Lianyungang 222005, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine, Biotechnology, Jiangsu Ocean University, Lianyungang 222005, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine, Biotechnology, Jiangsu Ocean University, Lianyungang 222005, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine, Biotechnology, Jiangsu Ocean University, Lianyungang 222005, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine, Biotechnology, Jiangsu Ocean University, Lianyungang 222005, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine, Biotechnology, Jiangsu Ocean University, Lianyungang 222005, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine, Biotechnology, Jiangsu Ocean University, Lianyungang 222005, ChinaDextranase is widely used in sugar production, drug synthesis, material preparation, and biotechnology, among other fields. The immobilization of dextranase using nanomaterials in order to make it reusable, is a hot research topic. In this study, the immobilization of purified dextranase was performed using different nanomaterials. The best results were obtained when dextranase was immobilized on titanium dioxide (TiO<sub>2</sub>), and a particle size of 30 nm was achieved. The optimum immobilization conditions were pH 7.0, temperature 25 °C, time 1 h, and immobilization agent TiO<sub>2</sub>. The immobilized materials were characterized using Fourier-transform infrared spectroscopy, X-ray diffractometry, and field emission gun scanning electron microscopy. The optimum temperature and pH of the immobilized dextranase were 30 °C and 7.5, respectively. The activity of the immobilized dextranase was >50% even after 7 times of reuse, and 58% of the enzyme was active even after 7 days of storage at 25 °C, indicating the reproducibility of the immobilized enzyme. The adsorption of dextranase by TiO<sub>2</sub> nanoparticles exhibited secondary reaction kinetics. Compared with free dextranase, the hydrolysates of the immobilized dextranase were significantly different, and consisted mainly of isomaltotriose and isomaltotetraose. The highly polymerized isomaltotetraose levels could reach >78.69% of the product after 30 min of enzymatic digestion.https://www.mdpi.com/2079-4991/13/6/1065dextranaseTiO<sub>2</sub> nanoparticlesimmobilizationhydrolysates |
| spellingShingle | Yingying Xu Huanyu Wang Qianru Lin Qingzhen Miao Mingwang Liu Hao Ni Lei Zhang Mingsheng Lyu Shujun Wang Immobilization of Dextranase Obtained from the Marine <i>Cellulosimicrobium</i> sp. Y1 on Nanoparticles: Nano-TiO<sub>2</sub> Improving Hydrolysate Properties and Enhancing Reuse Nanomaterials dextranase TiO<sub>2</sub> nanoparticles immobilization hydrolysates |
| title | Immobilization of Dextranase Obtained from the Marine <i>Cellulosimicrobium</i> sp. Y1 on Nanoparticles: Nano-TiO<sub>2</sub> Improving Hydrolysate Properties and Enhancing Reuse |
| title_full | Immobilization of Dextranase Obtained from the Marine <i>Cellulosimicrobium</i> sp. Y1 on Nanoparticles: Nano-TiO<sub>2</sub> Improving Hydrolysate Properties and Enhancing Reuse |
| title_fullStr | Immobilization of Dextranase Obtained from the Marine <i>Cellulosimicrobium</i> sp. Y1 on Nanoparticles: Nano-TiO<sub>2</sub> Improving Hydrolysate Properties and Enhancing Reuse |
| title_full_unstemmed | Immobilization of Dextranase Obtained from the Marine <i>Cellulosimicrobium</i> sp. Y1 on Nanoparticles: Nano-TiO<sub>2</sub> Improving Hydrolysate Properties and Enhancing Reuse |
| title_short | Immobilization of Dextranase Obtained from the Marine <i>Cellulosimicrobium</i> sp. Y1 on Nanoparticles: Nano-TiO<sub>2</sub> Improving Hydrolysate Properties and Enhancing Reuse |
| title_sort | immobilization of dextranase obtained from the marine i cellulosimicrobium i sp y1 on nanoparticles nano tio sub 2 sub improving hydrolysate properties and enhancing reuse |
| topic | dextranase TiO<sub>2</sub> nanoparticles immobilization hydrolysates |
| url | https://www.mdpi.com/2079-4991/13/6/1065 |
| work_keys_str_mv | AT yingyingxu immobilizationofdextranaseobtainedfromthemarineicellulosimicrobiumispy1onnanoparticlesnanotiosub2subimprovinghydrolysatepropertiesandenhancingreuse AT huanyuwang immobilizationofdextranaseobtainedfromthemarineicellulosimicrobiumispy1onnanoparticlesnanotiosub2subimprovinghydrolysatepropertiesandenhancingreuse AT qianrulin immobilizationofdextranaseobtainedfromthemarineicellulosimicrobiumispy1onnanoparticlesnanotiosub2subimprovinghydrolysatepropertiesandenhancingreuse AT qingzhenmiao immobilizationofdextranaseobtainedfromthemarineicellulosimicrobiumispy1onnanoparticlesnanotiosub2subimprovinghydrolysatepropertiesandenhancingreuse AT mingwangliu immobilizationofdextranaseobtainedfromthemarineicellulosimicrobiumispy1onnanoparticlesnanotiosub2subimprovinghydrolysatepropertiesandenhancingreuse AT haoni immobilizationofdextranaseobtainedfromthemarineicellulosimicrobiumispy1onnanoparticlesnanotiosub2subimprovinghydrolysatepropertiesandenhancingreuse AT leizhang immobilizationofdextranaseobtainedfromthemarineicellulosimicrobiumispy1onnanoparticlesnanotiosub2subimprovinghydrolysatepropertiesandenhancingreuse AT mingshenglyu immobilizationofdextranaseobtainedfromthemarineicellulosimicrobiumispy1onnanoparticlesnanotiosub2subimprovinghydrolysatepropertiesandenhancingreuse AT shujunwang immobilizationofdextranaseobtainedfromthemarineicellulosimicrobiumispy1onnanoparticlesnanotiosub2subimprovinghydrolysatepropertiesandenhancingreuse |