Screening and functional analysis of potential S genes in Chrysanthemum morifolium
S genes are the key genes that cause plant self-incompatibility, to find out the key S genes and understand molecular mechanism of self-incompatibility in chrysanthemum, the stigmas and anthers at different developmental stages of 'Q10-22-2'—a self-incompatible chrysanthemum cultivar, were...
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| Format: | Article |
| Language: | English |
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Maximum Academic Press
2021-01-01
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| Series: | Ornamental Plant Research |
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| Online Access: | https://www.maxapress.com/article/doi/10.48130/OPR-2021-0006 |
| _version_ | 1827339943548026880 |
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| author | Fan Wang Sujuan Xu Ze Wu Xinghua Zhong Weimin Fang Fadi Chen Nianjun Teng |
| author_facet | Fan Wang Sujuan Xu Ze Wu Xinghua Zhong Weimin Fang Fadi Chen Nianjun Teng |
| author_sort | Fan Wang |
| collection | DOAJ |
| description | S genes are the key genes that cause plant self-incompatibility, to find out the key S genes and understand molecular mechanism of self-incompatibility in chrysanthemum, the stigmas and anthers at different developmental stages of 'Q10-22-2'—a self-incompatible chrysanthemum cultivar, were used for RNA sequencing. After bioinformatics analysis, 13 candidate pistil S genes and five candidate pollen S genes were excavated. A potential pistil S gene was cloned and named as CmSRK1. Meanwhile, a potential pollen S gene was cloned and named as CmPCP1. qRT-PCR revealed that CmSRK1 was specifically expressed in mature stigmas, and CmPCP1 was specifically expressed in anthers 3 d before maturation. Subcellular localization showed that both CmSRK1 and CmPCP1 were located in the nucleus and the cell membrane. Transcriptional activation activity analysis indicated that both of the two proteins had no transcriptional activation activity. Yeast two hybrid assay showed that there was no interaction between CmSRK1 and CmPCP1. CmSRK1 was constructed on the expression vector containing stigma-specific promoter, and CmPCP1 was constructed on the expression vector containing pollen-specific promoter, they were then transformed into Arabidopsis thaliana. Artificial hybridization was performed with transgenic lines containing CmSRK1 as the female parents, and transgenic lines containing CmPCP1 as the male parents. The hybridization results showed that seed sets of two transgenic lines were 19.62% and 11.64%, respectively, while cross-pollinated seed sets of Col-0 was 84.43%. Therefore, it was speculated that CmSRK1 and CmPCP1 might be pistil and pollen S genes of chrysanthemum, respectively, and SI of chrysanthemum belonged to SSI. |
| first_indexed | 2024-03-07T20:09:53Z |
| format | Article |
| id | doaj.art-6684a0e560814b789d53753a65603e89 |
| institution | Directory Open Access Journal |
| issn | 2769-2094 |
| language | English |
| last_indexed | 2024-03-07T20:09:53Z |
| publishDate | 2021-01-01 |
| publisher | Maximum Academic Press |
| record_format | Article |
| series | Ornamental Plant Research |
| spelling | doaj.art-6684a0e560814b789d53753a65603e892024-02-28T01:52:27ZengMaximum Academic PressOrnamental Plant Research2769-20942021-01-011111110.48130/OPR-2021-0006OPR-2021-0006Screening and functional analysis of potential S genes in Chrysanthemum morifoliumFan Wang0Sujuan Xu1Ze Wu2Xinghua Zhong3Weimin Fang4Fadi Chen5Nianjun Teng6College of Horticulture, Nanjing Agricultural University, Key Laboratory of Landscape Design, Ministry of Agriculture and Rural Affairs, Nanjing 210095, ChinaCollege of Horticulture, Nanjing Agricultural University, Key Laboratory of Landscape Design, Ministry of Agriculture and Rural Affairs, Nanjing 210095, ChinaCollege of Horticulture, Nanjing Agricultural University, Key Laboratory of Landscape Design, Ministry of Agriculture and Rural Affairs, Nanjing 210095, ChinaCollege of Horticulture, Nanjing Agricultural University, Key Laboratory of Landscape Design, Ministry of Agriculture and Rural Affairs, Nanjing 210095, ChinaCollege of Horticulture, Nanjing Agricultural University, Key Laboratory of Landscape Design, Ministry of Agriculture and Rural Affairs, Nanjing 210095, ChinaCollege of Horticulture, Nanjing Agricultural University, Key Laboratory of Landscape Design, Ministry of Agriculture and Rural Affairs, Nanjing 210095, ChinaCollege of Horticulture, Nanjing Agricultural University, Key Laboratory of Landscape Design, Ministry of Agriculture and Rural Affairs, Nanjing 210095, ChinaS genes are the key genes that cause plant self-incompatibility, to find out the key S genes and understand molecular mechanism of self-incompatibility in chrysanthemum, the stigmas and anthers at different developmental stages of 'Q10-22-2'—a self-incompatible chrysanthemum cultivar, were used for RNA sequencing. After bioinformatics analysis, 13 candidate pistil S genes and five candidate pollen S genes were excavated. A potential pistil S gene was cloned and named as CmSRK1. Meanwhile, a potential pollen S gene was cloned and named as CmPCP1. qRT-PCR revealed that CmSRK1 was specifically expressed in mature stigmas, and CmPCP1 was specifically expressed in anthers 3 d before maturation. Subcellular localization showed that both CmSRK1 and CmPCP1 were located in the nucleus and the cell membrane. Transcriptional activation activity analysis indicated that both of the two proteins had no transcriptional activation activity. Yeast two hybrid assay showed that there was no interaction between CmSRK1 and CmPCP1. CmSRK1 was constructed on the expression vector containing stigma-specific promoter, and CmPCP1 was constructed on the expression vector containing pollen-specific promoter, they were then transformed into Arabidopsis thaliana. Artificial hybridization was performed with transgenic lines containing CmSRK1 as the female parents, and transgenic lines containing CmPCP1 as the male parents. The hybridization results showed that seed sets of two transgenic lines were 19.62% and 11.64%, respectively, while cross-pollinated seed sets of Col-0 was 84.43%. Therefore, it was speculated that CmSRK1 and CmPCP1 might be pistil and pollen S genes of chrysanthemum, respectively, and SI of chrysanthemum belonged to SSI.https://www.maxapress.com/article/doi/10.48130/OPR-2021-0006chrysanthemum morifoliums geneself-incompatibilityrna-seqcmsrk1cmpcp1 |
| spellingShingle | Fan Wang Sujuan Xu Ze Wu Xinghua Zhong Weimin Fang Fadi Chen Nianjun Teng Screening and functional analysis of potential S genes in Chrysanthemum morifolium Ornamental Plant Research chrysanthemum morifolium s gene self-incompatibility rna-seq cmsrk1 cmpcp1 |
| title | Screening and functional analysis of potential S genes in Chrysanthemum morifolium |
| title_full | Screening and functional analysis of potential S genes in Chrysanthemum morifolium |
| title_fullStr | Screening and functional analysis of potential S genes in Chrysanthemum morifolium |
| title_full_unstemmed | Screening and functional analysis of potential S genes in Chrysanthemum morifolium |
| title_short | Screening and functional analysis of potential S genes in Chrysanthemum morifolium |
| title_sort | screening and functional analysis of potential s genes in chrysanthemum morifolium |
| topic | chrysanthemum morifolium s gene self-incompatibility rna-seq cmsrk1 cmpcp1 |
| url | https://www.maxapress.com/article/doi/10.48130/OPR-2021-0006 |
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