An Immunosensor for the Determination of Cortisol in Serum and Saliva by Array SPRi
Cortisol is a hormone which plays an essential role in the immune, endocrine, cardiovascular, renal and skeletal systems. Its level increases in response to stress, illness, injury or exhaustion, and it is therefore a significant diagnostic biomarker of stress. An immunosensor for the determination...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/22/24/9675 |
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| author | Anna Sankiewicz Lukasz Oldak Beata Zelazowska-Rutkowska Adam Hermanowicz Zenon Lukaszewski Ewa Gorodkiewicz |
| author_facet | Anna Sankiewicz Lukasz Oldak Beata Zelazowska-Rutkowska Adam Hermanowicz Zenon Lukaszewski Ewa Gorodkiewicz |
| author_sort | Anna Sankiewicz |
| collection | DOAJ |
| description | Cortisol is a hormone which plays an essential role in the immune, endocrine, cardiovascular, renal and skeletal systems. Its level increases in response to stress, illness, injury or exhaustion, and it is therefore a significant diagnostic biomarker of stress. An immunosensor for the determination of cortisol by SPRi array was developed. The receptive part of the immunosensor is mouse monoclonal antibody against cortisol, immobilized via cysteamine linker. The optimum pH of the immunosensor is 7.4, and the optimum concentration of the antibody is 50 ng mL<sup>−1</sup>. The immunosensor is specific for cortisol, and its linear response ranges from 0.20 ng mL<sup>−1</sup> (LOQ) to 8 ng mL<sup>−1</sup>. The precision of the determination was between 3.1% and 3.3%, and the recovery between 99% and 102%. The immunosensor was validated by simultaneous determination of cortisol in serum and saliva samples by a standard method, with good agreement between the results. |
| first_indexed | 2024-03-09T15:52:34Z |
| format | Article |
| id | doaj.art-668b6d2b9c424f61aa2782d3741e44fb |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-09T15:52:34Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
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| series | Sensors |
| spelling | doaj.art-668b6d2b9c424f61aa2782d3741e44fb2023-11-24T17:53:36ZengMDPI AGSensors1424-82202022-12-012224967510.3390/s22249675An Immunosensor for the Determination of Cortisol in Serum and Saliva by Array SPRiAnna Sankiewicz0Lukasz Oldak1Beata Zelazowska-Rutkowska2Adam Hermanowicz3Zenon Lukaszewski4Ewa Gorodkiewicz5Bioanalysis Laboratory, Faculty of Chemistry, University of Bialystok, Ciolkowskiego 1K, 15-245 Bialystok, PolandBioanalysis Laboratory, Faculty of Chemistry, University of Bialystok, Ciolkowskiego 1K, 15-245 Bialystok, PolandDepartment of Pediatric Laboratory Diagnostics, Medical University of Bialystok, Waszyngtona 17, 15-274 Bialystok, PolandDepartment of Pediatric Surgery and Urology, Medical University of Bialystok, Waszyngtona 17, 15-274 Bialystok, PolandFaculty of Chemical Technology, Poznan University of Technology, 5 M. Skłodowska-Curie Square, 60-965 Poznan, PolandBioanalysis Laboratory, Faculty of Chemistry, University of Bialystok, Ciolkowskiego 1K, 15-245 Bialystok, PolandCortisol is a hormone which plays an essential role in the immune, endocrine, cardiovascular, renal and skeletal systems. Its level increases in response to stress, illness, injury or exhaustion, and it is therefore a significant diagnostic biomarker of stress. An immunosensor for the determination of cortisol by SPRi array was developed. The receptive part of the immunosensor is mouse monoclonal antibody against cortisol, immobilized via cysteamine linker. The optimum pH of the immunosensor is 7.4, and the optimum concentration of the antibody is 50 ng mL<sup>−1</sup>. The immunosensor is specific for cortisol, and its linear response ranges from 0.20 ng mL<sup>−1</sup> (LOQ) to 8 ng mL<sup>−1</sup>. The precision of the determination was between 3.1% and 3.3%, and the recovery between 99% and 102%. The immunosensor was validated by simultaneous determination of cortisol in serum and saliva samples by a standard method, with good agreement between the results.https://www.mdpi.com/1424-8220/22/24/9675SPRi arrayQCMserumsalivacortisol |
| spellingShingle | Anna Sankiewicz Lukasz Oldak Beata Zelazowska-Rutkowska Adam Hermanowicz Zenon Lukaszewski Ewa Gorodkiewicz An Immunosensor for the Determination of Cortisol in Serum and Saliva by Array SPRi Sensors SPRi array QCM serum saliva cortisol |
| title | An Immunosensor for the Determination of Cortisol in Serum and Saliva by Array SPRi |
| title_full | An Immunosensor for the Determination of Cortisol in Serum and Saliva by Array SPRi |
| title_fullStr | An Immunosensor for the Determination of Cortisol in Serum and Saliva by Array SPRi |
| title_full_unstemmed | An Immunosensor for the Determination of Cortisol in Serum and Saliva by Array SPRi |
| title_short | An Immunosensor for the Determination of Cortisol in Serum and Saliva by Array SPRi |
| title_sort | immunosensor for the determination of cortisol in serum and saliva by array spri |
| topic | SPRi array QCM serum saliva cortisol |
| url | https://www.mdpi.com/1424-8220/22/24/9675 |
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