The Potential of <i>Pediococcus acidilactici</i> Cell-Free Supernatant as a Preservative in Food Packaging Materials
This study delves into the production and antimicrobial characteristics of cell-free supernatants from <i>Pediococcus acidilactici</i> (CFSs-Pa). Antimicrobial activity was initially observed in CFS-Pa harvested after 12 h of incubation and increased up to the late stationary phase at 48...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-02-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/13/5/644 |
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| author | Katherine Kho Adinda Darwanti Kadar Mario Donald Bani Ihsan Tria Pramanda Leon Martin Matthew Chrisdianto Ferren Pratama Putu Virgina Partha Devanthi |
| author_facet | Katherine Kho Adinda Darwanti Kadar Mario Donald Bani Ihsan Tria Pramanda Leon Martin Matthew Chrisdianto Ferren Pratama Putu Virgina Partha Devanthi |
| author_sort | Katherine Kho |
| collection | DOAJ |
| description | This study delves into the production and antimicrobial characteristics of cell-free supernatants from <i>Pediococcus acidilactici</i> (CFSs-Pa). Antimicrobial activity was initially observed in CFS-Pa harvested after 12 h of incubation and increased up to the late stationary phase at 48 h. The increase in antimicrobial activity did not align with total protein content, pointing to other factors linked to the accumulation of organic acids, particularly lactic acid. The SDS-PAGE analysis also indicated that the expected proteinaceous compound (pediocin) was not observed in CFS-Pa. Further investigations suggested that the antimicrobial properties of CFS-Pa were exclusively due to organic acids. The MIC values confirmed potent antimicrobial activity, particularly at a 10% dilution of CFS-Pa in MRS broth. The time–kill assays demonstrated bactericidal activity against EHEC, <i>Listeria monocytogenes,</i> and <i>Staphylococcus aureus</i> by 12 h, 18 h, and 24 h using a 10% dilution of CFS-Pa. Additionally, CFS-Pa exhibited dose-dependent antioxidant activity, requiring a 70% (<i>v</i>/<i>v</i>) concentration to inhibit DPPH scavenging activity by 50%. All the experimental results suggested potential applications of CFS-Pa in food preservation. An attempt to incorporate CFS-Pa into bacterial cellulose (BC) for edible food packaging demonstrated promising antimicrobial results, particularly against <i>L. monocytogenes</i> and <i>S. aureus</i>, with room for optimization. |
| first_indexed | 2024-04-25T00:31:08Z |
| format | Article |
| id | doaj.art-66950f1e3ee2437faccb81efae3c4e32 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-04-25T00:31:08Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-66950f1e3ee2437faccb81efae3c4e322024-03-12T16:43:59ZengMDPI AGFoods2304-81582024-02-0113564410.3390/foods13050644The Potential of <i>Pediococcus acidilactici</i> Cell-Free Supernatant as a Preservative in Food Packaging MaterialsKatherine Kho0Adinda Darwanti Kadar1Mario Donald Bani2Ihsan Tria Pramanda3Leon Martin4Matthew Chrisdianto5Ferren Pratama6Putu Virgina Partha Devanthi7Department of Biotechnology, Faculty of Life Sciences, Indonesia International Institute for Life Sciences, Pulomas Barat Kavling 88, Jakarta 13210, IndonesiaDepartment of Biotechnology, Faculty of Life Sciences, Indonesia International Institute for Life Sciences, Pulomas Barat Kavling 88, Jakarta 13210, IndonesiaDepartment of Biotechnology, Faculty of Life Sciences, Indonesia International Institute for Life Sciences, Pulomas Barat Kavling 88, Jakarta 13210, IndonesiaDepartment of Biotechnology, Faculty of Life Sciences, Indonesia International Institute for Life Sciences, Pulomas Barat Kavling 88, Jakarta 13210, IndonesiaDepartment of Biotechnology, Faculty of Life Sciences, Indonesia International Institute for Life Sciences, Pulomas Barat Kavling 88, Jakarta 13210, IndonesiaDepartment of Biotechnology, Faculty of Life Sciences, Indonesia International Institute for Life Sciences, Pulomas Barat Kavling 88, Jakarta 13210, IndonesiaDepartment of Biotechnology, Faculty of Life Sciences, Indonesia International Institute for Life Sciences, Pulomas Barat Kavling 88, Jakarta 13210, IndonesiaDepartment of Biotechnology, Faculty of Life Sciences, Indonesia International Institute for Life Sciences, Pulomas Barat Kavling 88, Jakarta 13210, IndonesiaThis study delves into the production and antimicrobial characteristics of cell-free supernatants from <i>Pediococcus acidilactici</i> (CFSs-Pa). Antimicrobial activity was initially observed in CFS-Pa harvested after 12 h of incubation and increased up to the late stationary phase at 48 h. The increase in antimicrobial activity did not align with total protein content, pointing to other factors linked to the accumulation of organic acids, particularly lactic acid. The SDS-PAGE analysis also indicated that the expected proteinaceous compound (pediocin) was not observed in CFS-Pa. Further investigations suggested that the antimicrobial properties of CFS-Pa were exclusively due to organic acids. The MIC values confirmed potent antimicrobial activity, particularly at a 10% dilution of CFS-Pa in MRS broth. The time–kill assays demonstrated bactericidal activity against EHEC, <i>Listeria monocytogenes,</i> and <i>Staphylococcus aureus</i> by 12 h, 18 h, and 24 h using a 10% dilution of CFS-Pa. Additionally, CFS-Pa exhibited dose-dependent antioxidant activity, requiring a 70% (<i>v</i>/<i>v</i>) concentration to inhibit DPPH scavenging activity by 50%. All the experimental results suggested potential applications of CFS-Pa in food preservation. An attempt to incorporate CFS-Pa into bacterial cellulose (BC) for edible food packaging demonstrated promising antimicrobial results, particularly against <i>L. monocytogenes</i> and <i>S. aureus</i>, with room for optimization.https://www.mdpi.com/2304-8158/13/5/644<i>Pediococcus acidilactici</i>cell-free supernatantpostbioticsorganic acidsfood packagingnatural preservatives |
| spellingShingle | Katherine Kho Adinda Darwanti Kadar Mario Donald Bani Ihsan Tria Pramanda Leon Martin Matthew Chrisdianto Ferren Pratama Putu Virgina Partha Devanthi The Potential of <i>Pediococcus acidilactici</i> Cell-Free Supernatant as a Preservative in Food Packaging Materials Foods <i>Pediococcus acidilactici</i> cell-free supernatant postbiotics organic acids food packaging natural preservatives |
| title | The Potential of <i>Pediococcus acidilactici</i> Cell-Free Supernatant as a Preservative in Food Packaging Materials |
| title_full | The Potential of <i>Pediococcus acidilactici</i> Cell-Free Supernatant as a Preservative in Food Packaging Materials |
| title_fullStr | The Potential of <i>Pediococcus acidilactici</i> Cell-Free Supernatant as a Preservative in Food Packaging Materials |
| title_full_unstemmed | The Potential of <i>Pediococcus acidilactici</i> Cell-Free Supernatant as a Preservative in Food Packaging Materials |
| title_short | The Potential of <i>Pediococcus acidilactici</i> Cell-Free Supernatant as a Preservative in Food Packaging Materials |
| title_sort | potential of i pediococcus acidilactici i cell free supernatant as a preservative in food packaging materials |
| topic | <i>Pediococcus acidilactici</i> cell-free supernatant postbiotics organic acids food packaging natural preservatives |
| url | https://www.mdpi.com/2304-8158/13/5/644 |
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