Anticancer potentials of metformin loaded coconut oil nanoemulsion on MCF-7, HepG2 and HCT-116 cell lines
AbstractIn order to load metformin in a nano formula and evaluate the produced nano form towards cancer cells, metformin was loaded on natural carrier coconut oil. The formed metformin-loaded coconut oil nanoemulsion was characterized by Zeta potential, particle size, drug content, drug release, and...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2023-12-01
|
| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2023.2246145 |
| _version_ | 1797631191650664448 |
|---|---|
| author | Hadil Faris Alotaibi El-Sayed Khafagy Amr Selim Abu Lila Haifa F. Alotaibe Serag Eldin Elbehairi Ashwag S. Alanazi Mohammad Y. Alfaifi Jawaher Abdullah Alamoudi Sarah Salem Alamrani Fatma Alzahraa Mokhtar |
| author_facet | Hadil Faris Alotaibi El-Sayed Khafagy Amr Selim Abu Lila Haifa F. Alotaibe Serag Eldin Elbehairi Ashwag S. Alanazi Mohammad Y. Alfaifi Jawaher Abdullah Alamoudi Sarah Salem Alamrani Fatma Alzahraa Mokhtar |
| author_sort | Hadil Faris Alotaibi |
| collection | DOAJ |
| description | AbstractIn order to load metformin in a nano formula and evaluate the produced nano form towards cancer cells, metformin was loaded on natural carrier coconut oil. The formed metformin-loaded coconut oil nanoemulsion was characterized by Zeta potential, particle size, drug content, drug release, and drug stability. The formed nanoemulsion was evaluated towards MCF-7, HepG2, and HCT-116 cell lines. Cell cycle analysis and apoptosis mechanism were studied. The nanoemulsion was created using deionized water, 1.5% Span 20, 1.5% Tween 80, 1.5% coconut oil, and 0.5% Metformin in an ultrasonicator to produce a homogenous solution. The anticancer activities of the metformin-loaded coconut nanoemulsion were highly improved compared to non-formulated metformin with IC50s of 8.3 ± 0.1 µg/ml, 12 ± 1.5 µg/ml, 2.685 ± 0.3 µg/ml for MCF-7, HepG2, and HCT-116 cell lines, respectively. There was a 76.5 ± 2.3 and 78.3 ± 3.2% increase in the number of apoptotic cells of MCF-7 and HepG2 cells after nanoemulsion treatment. This formula may be considered a new anticancer medication. |
| first_indexed | 2024-03-11T11:17:28Z |
| format | Article |
| id | doaj.art-6695e619e79745239150c9968593d685 |
| institution | Directory Open Access Journal |
| issn | 2169-1401 2169-141X |
| language | English |
| last_indexed | 2024-03-11T11:17:28Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj.art-6695e619e79745239150c9968593d6852023-11-10T17:19:33ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2023-12-0151141942710.1080/21691401.2023.2246145Anticancer potentials of metformin loaded coconut oil nanoemulsion on MCF-7, HepG2 and HCT-116 cell linesHadil Faris Alotaibi0El-Sayed Khafagy1Amr Selim Abu Lila2Haifa F. Alotaibe3Serag Eldin Elbehairi4Ashwag S. Alanazi5Mohammad Y. Alfaifi6Jawaher Abdullah Alamoudi7Sarah Salem Alamrani8Fatma Alzahraa Mokhtar9Department of Pharmaceutical Sciences, College of Pharmacy, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-kharj, Saudi ArabiaDepartment of Pharmaceutics, College of Pharmacy, University of Hail, Hail, Saudi ArabiaDepartment of Family Medicine, Prince Sultan Military Medical City, Riyadh, Saudi ArabiaFaculty of science, Biology Department, King Khalid University, Abha, Saudi ArabiaDepartment of Pharmaceutical Sciences, College of Pharmacy, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi ArabiaFaculty of science, Biology Department, King Khalid University, Abha, Saudi ArabiaDepartment of Pharmaceutical Sciences, College of Pharmacy, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi ArabiaCollege of Pharmacy, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi ArabiaDepartment of pharmacognosy, El Saleheya El Gadida University, El Saleheya El Gadida, Sharkia, EgyptAbstractIn order to load metformin in a nano formula and evaluate the produced nano form towards cancer cells, metformin was loaded on natural carrier coconut oil. The formed metformin-loaded coconut oil nanoemulsion was characterized by Zeta potential, particle size, drug content, drug release, and drug stability. The formed nanoemulsion was evaluated towards MCF-7, HepG2, and HCT-116 cell lines. Cell cycle analysis and apoptosis mechanism were studied. The nanoemulsion was created using deionized water, 1.5% Span 20, 1.5% Tween 80, 1.5% coconut oil, and 0.5% Metformin in an ultrasonicator to produce a homogenous solution. The anticancer activities of the metformin-loaded coconut nanoemulsion were highly improved compared to non-formulated metformin with IC50s of 8.3 ± 0.1 µg/ml, 12 ± 1.5 µg/ml, 2.685 ± 0.3 µg/ml for MCF-7, HepG2, and HCT-116 cell lines, respectively. There was a 76.5 ± 2.3 and 78.3 ± 3.2% increase in the number of apoptotic cells of MCF-7 and HepG2 cells after nanoemulsion treatment. This formula may be considered a new anticancer medication.https://www.tandfonline.com/doi/10.1080/21691401.2023.2246145mcf-7hct-116HepG2nanoemulsionmetforminanticancer |
| spellingShingle | Hadil Faris Alotaibi El-Sayed Khafagy Amr Selim Abu Lila Haifa F. Alotaibe Serag Eldin Elbehairi Ashwag S. Alanazi Mohammad Y. Alfaifi Jawaher Abdullah Alamoudi Sarah Salem Alamrani Fatma Alzahraa Mokhtar Anticancer potentials of metformin loaded coconut oil nanoemulsion on MCF-7, HepG2 and HCT-116 cell lines Artificial Cells, Nanomedicine, and Biotechnology mcf-7 hct-116 HepG2 nanoemulsion metformin anticancer |
| title | Anticancer potentials of metformin loaded coconut oil nanoemulsion on MCF-7, HepG2 and HCT-116 cell lines |
| title_full | Anticancer potentials of metformin loaded coconut oil nanoemulsion on MCF-7, HepG2 and HCT-116 cell lines |
| title_fullStr | Anticancer potentials of metformin loaded coconut oil nanoemulsion on MCF-7, HepG2 and HCT-116 cell lines |
| title_full_unstemmed | Anticancer potentials of metformin loaded coconut oil nanoemulsion on MCF-7, HepG2 and HCT-116 cell lines |
| title_short | Anticancer potentials of metformin loaded coconut oil nanoemulsion on MCF-7, HepG2 and HCT-116 cell lines |
| title_sort | anticancer potentials of metformin loaded coconut oil nanoemulsion on mcf 7 hepg2 and hct 116 cell lines |
| topic | mcf-7 hct-116 HepG2 nanoemulsion metformin anticancer |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2023.2246145 |
| work_keys_str_mv | AT hadilfarisalotaibi anticancerpotentialsofmetforminloadedcoconutoilnanoemulsiononmcf7hepg2andhct116celllines AT elsayedkhafagy anticancerpotentialsofmetforminloadedcoconutoilnanoemulsiononmcf7hepg2andhct116celllines AT amrselimabulila anticancerpotentialsofmetforminloadedcoconutoilnanoemulsiononmcf7hepg2andhct116celllines AT haifafalotaibe anticancerpotentialsofmetforminloadedcoconutoilnanoemulsiononmcf7hepg2andhct116celllines AT serageldinelbehairi anticancerpotentialsofmetforminloadedcoconutoilnanoemulsiononmcf7hepg2andhct116celllines AT ashwagsalanazi anticancerpotentialsofmetforminloadedcoconutoilnanoemulsiononmcf7hepg2andhct116celllines AT mohammadyalfaifi anticancerpotentialsofmetforminloadedcoconutoilnanoemulsiononmcf7hepg2andhct116celllines AT jawaherabdullahalamoudi anticancerpotentialsofmetforminloadedcoconutoilnanoemulsiononmcf7hepg2andhct116celllines AT sarahsalemalamrani anticancerpotentialsofmetforminloadedcoconutoilnanoemulsiononmcf7hepg2andhct116celllines AT fatmaalzahraamokhtar anticancerpotentialsofmetforminloadedcoconutoilnanoemulsiononmcf7hepg2andhct116celllines |