Differential Expression of <i>AhR</i> in Peripheral Mononuclear Cells in Response to Exposure to Polycyclic Aromatic Hydrocarbons in Mexican Women
The exposure to air pollutants causes significant damage to health, and inefficient cooking and heating practices produce high levels of household air pollution, including a wide range of health-damaging pollutants such as fine particles, carbon monoxide and PAHs. The exposure to PAHs has been assoc...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Toxics |
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| Online Access: | https://www.mdpi.com/2305-6304/11/1/28 |
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| author | José Antonio Varela-Silva Miguel Ernesto Martínez-Leija Sandra Teresa Orta-García Ivan Nelinho Pérez-Maldonado Jesús Adrián López Hiram Hernández-López Roberto González-Amaro Emma S. Calderón-Aranda Diana Patricia Portales-Pérez Mariana Salgado-Bustamante |
| author_facet | José Antonio Varela-Silva Miguel Ernesto Martínez-Leija Sandra Teresa Orta-García Ivan Nelinho Pérez-Maldonado Jesús Adrián López Hiram Hernández-López Roberto González-Amaro Emma S. Calderón-Aranda Diana Patricia Portales-Pérez Mariana Salgado-Bustamante |
| author_sort | José Antonio Varela-Silva |
| collection | DOAJ |
| description | The exposure to air pollutants causes significant damage to health, and inefficient cooking and heating practices produce high levels of household air pollution, including a wide range of health-damaging pollutants such as fine particles, carbon monoxide and PAHs. The exposure to PAHs has been associated with the development of neoplastic processes, asthma, genotoxicity, altered neurodevelopment and inflammation. The effects on the induction of proinflammatory cytokines are attributed to the activation of <i>AhR</i>. However, the molecular mechanisms by which the PAHs produce proinflammatory effects are unknown. This study was performed on a group of 41 Mexican women from two rural communities who had stoves inside their houses, used wood as biomass fuel, and, thus, were vulnerable. According to the urinary 1-OHP concentration, the samples were stratified into two groups for determination of the levels of <i>TNF-α</i>, <i>AhR</i>, <i>CYP1B1</i>, miR-125b and miR-155 expression. Our results showed that the <i>CYP1B1</i>, <i>TNF-α</i>, miR-125b and miR-155 expression levels were not statistically different between women with the lowest and highest levels of 1-OHP. Interestingly, high levels of PAHs promoted augmented expression of AhR, which is a protein involved in the modulation of inflammatory pathways in vivo, suggesting that cell signaling of <i>AhR</i> may be implicated in several pathogenesis processes. |
| first_indexed | 2024-03-09T11:07:05Z |
| format | Article |
| id | doaj.art-6696e9c7b04a4e4f95bde5abecc1a193 |
| institution | Directory Open Access Journal |
| issn | 2305-6304 |
| language | English |
| last_indexed | 2024-03-09T11:07:05Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxics |
| spelling | doaj.art-6696e9c7b04a4e4f95bde5abecc1a1932023-12-01T00:55:21ZengMDPI AGToxics2305-63042022-12-011112810.3390/toxics11010028Differential Expression of <i>AhR</i> in Peripheral Mononuclear Cells in Response to Exposure to Polycyclic Aromatic Hydrocarbons in Mexican WomenJosé Antonio Varela-Silva0Miguel Ernesto Martínez-Leija1Sandra Teresa Orta-García2Ivan Nelinho Pérez-Maldonado3Jesús Adrián López4Hiram Hernández-López5Roberto González-Amaro6Emma S. Calderón-Aranda7Diana Patricia Portales-Pérez8Mariana Salgado-Bustamante9Biochemistry Department, and Immunology, Department of Faculty of Medicine, UASLP, San Luis Potosí 78000, MexicoBiochemistry Department, and Immunology, Department of Faculty of Medicine, UASLP, San Luis Potosí 78000, MexicoLaboratory of Molecular Toxicology, Centro de Investigación Aplicada en, Ambiente y Salud (CIAAS), Coordinación para la Innovación y Aplicación de la, Ciencia y la Tecnología (CIACYT), UASLP, San Luis Potosí 78000, MexicoLaboratory of Molecular Toxicology, Centro de Investigación Aplicada en, Ambiente y Salud (CIAAS), Coordinación para la Innovación y Aplicación de la, Ciencia y la Tecnología (CIACYT), UASLP, San Luis Potosí 78000, MexicoLaboratorio de microRNAs y Cáncer, Unidad Académica de Ciencias Biológicas, Universidad Autónoma de Zacatecas, Zacatecas 98066, MexicoAcademic Unit of Chemistry Sciences, Universidad Autónoma de Zacatecas, Zacatecas 98606, MexicoLaboratory of Immunology Cellular and molecular, Faculty of Chemistry, Universidad Autónoma de San Luis Potosí, San Luis Potosí 78000, MexicoToxicology Department, Centro de Investigación y Estudios Avanzados, IPN, México City 07360, MexicoLaboratorio de microRNAs y Cáncer, Unidad Académica de Ciencias Biológicas, Universidad Autónoma de Zacatecas, Zacatecas 98066, MexicoBiochemistry Department, and Immunology, Department of Faculty of Medicine, UASLP, San Luis Potosí 78000, MexicoThe exposure to air pollutants causes significant damage to health, and inefficient cooking and heating practices produce high levels of household air pollution, including a wide range of health-damaging pollutants such as fine particles, carbon monoxide and PAHs. The exposure to PAHs has been associated with the development of neoplastic processes, asthma, genotoxicity, altered neurodevelopment and inflammation. The effects on the induction of proinflammatory cytokines are attributed to the activation of <i>AhR</i>. However, the molecular mechanisms by which the PAHs produce proinflammatory effects are unknown. This study was performed on a group of 41 Mexican women from two rural communities who had stoves inside their houses, used wood as biomass fuel, and, thus, were vulnerable. According to the urinary 1-OHP concentration, the samples were stratified into two groups for determination of the levels of <i>TNF-α</i>, <i>AhR</i>, <i>CYP1B1</i>, miR-125b and miR-155 expression. Our results showed that the <i>CYP1B1</i>, <i>TNF-α</i>, miR-125b and miR-155 expression levels were not statistically different between women with the lowest and highest levels of 1-OHP. Interestingly, high levels of PAHs promoted augmented expression of AhR, which is a protein involved in the modulation of inflammatory pathways in vivo, suggesting that cell signaling of <i>AhR</i> may be implicated in several pathogenesis processes.https://www.mdpi.com/2305-6304/11/1/28pollutantswomen exposuremicroRNAsPAHs |
| spellingShingle | José Antonio Varela-Silva Miguel Ernesto Martínez-Leija Sandra Teresa Orta-García Ivan Nelinho Pérez-Maldonado Jesús Adrián López Hiram Hernández-López Roberto González-Amaro Emma S. Calderón-Aranda Diana Patricia Portales-Pérez Mariana Salgado-Bustamante Differential Expression of <i>AhR</i> in Peripheral Mononuclear Cells in Response to Exposure to Polycyclic Aromatic Hydrocarbons in Mexican Women Toxics pollutants women exposure microRNAs PAHs |
| title | Differential Expression of <i>AhR</i> in Peripheral Mononuclear Cells in Response to Exposure to Polycyclic Aromatic Hydrocarbons in Mexican Women |
| title_full | Differential Expression of <i>AhR</i> in Peripheral Mononuclear Cells in Response to Exposure to Polycyclic Aromatic Hydrocarbons in Mexican Women |
| title_fullStr | Differential Expression of <i>AhR</i> in Peripheral Mononuclear Cells in Response to Exposure to Polycyclic Aromatic Hydrocarbons in Mexican Women |
| title_full_unstemmed | Differential Expression of <i>AhR</i> in Peripheral Mononuclear Cells in Response to Exposure to Polycyclic Aromatic Hydrocarbons in Mexican Women |
| title_short | Differential Expression of <i>AhR</i> in Peripheral Mononuclear Cells in Response to Exposure to Polycyclic Aromatic Hydrocarbons in Mexican Women |
| title_sort | differential expression of i ahr i in peripheral mononuclear cells in response to exposure to polycyclic aromatic hydrocarbons in mexican women |
| topic | pollutants women exposure microRNAs PAHs |
| url | https://www.mdpi.com/2305-6304/11/1/28 |
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