Transcriptome profiling analysis reveals key genes of different coat color in sheep skin
Background To investigate the molecular mechanisms determining the coat color of native breed sheep in Xinjiang. Methods Bashibai sheep, Yemule white sheep and Tulufan black sheep were selected. Illumina HiSeq X Ten sequencing technology was used to detect the genes responsible for the white, light...
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| Format: | Article |
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PeerJ Inc.
2019-11-01
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| Series: | PeerJ |
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| Online Access: | https://peerj.com/articles/8077.pdf |
| _version_ | 1797420898531147776 |
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| author | Lidan Yao Aodungerile Bao Wenjuan Hong Chenxi Hou Zhenliang Zhang Xiaopeng Liang Jueken Aniwashi |
| author_facet | Lidan Yao Aodungerile Bao Wenjuan Hong Chenxi Hou Zhenliang Zhang Xiaopeng Liang Jueken Aniwashi |
| author_sort | Lidan Yao |
| collection | DOAJ |
| description | Background To investigate the molecular mechanisms determining the coat color of native breed sheep in Xinjiang. Methods Bashibai sheep, Yemule white sheep and Tulufan black sheep were selected. Illumina HiSeq X Ten sequencing technology was used to detect the genes responsible for the white, light brown, black and cyan gray coat colors in sheep. Sequence analysis and functional gene annotation analysis were performed to analyze the results. The signal pathways and differentially expressed genes related to sheep hair color production regulation were screened and finally verified by real-time polymerase chain reaction. Results Functional annotation by Kyoto Encyclopedia of Genes and Genomes analysis revealed significant differences in enrichment of immunity-related pathways as well as melanogenesis synthetic and tyrosine metabolism pathways. Our results showed that the DCT, TYR, TYRP1, PMEL, SLC45A2 and MLANA six genes may be associated with the regulation of coat color development and provide a theoretical basis for selecting natural coat colors of sheep. |
| first_indexed | 2024-03-09T07:08:19Z |
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| id | doaj.art-66988a87e7c741fc96e69bf19e3cd312 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:08:19Z |
| publishDate | 2019-11-01 |
| publisher | PeerJ Inc. |
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| spelling | doaj.art-66988a87e7c741fc96e69bf19e3cd3122023-12-03T09:19:20ZengPeerJ Inc.PeerJ2167-83592019-11-017e807710.7717/peerj.8077Transcriptome profiling analysis reveals key genes of different coat color in sheep skinLidan YaoAodungerile BaoWenjuan HongChenxi HouZhenliang ZhangXiaopeng LiangJueken AniwashiBackground To investigate the molecular mechanisms determining the coat color of native breed sheep in Xinjiang. Methods Bashibai sheep, Yemule white sheep and Tulufan black sheep were selected. Illumina HiSeq X Ten sequencing technology was used to detect the genes responsible for the white, light brown, black and cyan gray coat colors in sheep. Sequence analysis and functional gene annotation analysis were performed to analyze the results. The signal pathways and differentially expressed genes related to sheep hair color production regulation were screened and finally verified by real-time polymerase chain reaction. Results Functional annotation by Kyoto Encyclopedia of Genes and Genomes analysis revealed significant differences in enrichment of immunity-related pathways as well as melanogenesis synthetic and tyrosine metabolism pathways. Our results showed that the DCT, TYR, TYRP1, PMEL, SLC45A2 and MLANA six genes may be associated with the regulation of coat color development and provide a theoretical basis for selecting natural coat colors of sheep.https://peerj.com/articles/8077.pdfSheepRNA-seqMolecular mechanismCoat color |
| spellingShingle | Lidan Yao Aodungerile Bao Wenjuan Hong Chenxi Hou Zhenliang Zhang Xiaopeng Liang Jueken Aniwashi Transcriptome profiling analysis reveals key genes of different coat color in sheep skin PeerJ Sheep RNA-seq Molecular mechanism Coat color |
| title | Transcriptome profiling analysis reveals key genes of different coat color in sheep skin |
| title_full | Transcriptome profiling analysis reveals key genes of different coat color in sheep skin |
| title_fullStr | Transcriptome profiling analysis reveals key genes of different coat color in sheep skin |
| title_full_unstemmed | Transcriptome profiling analysis reveals key genes of different coat color in sheep skin |
| title_short | Transcriptome profiling analysis reveals key genes of different coat color in sheep skin |
| title_sort | transcriptome profiling analysis reveals key genes of different coat color in sheep skin |
| topic | Sheep RNA-seq Molecular mechanism Coat color |
| url | https://peerj.com/articles/8077.pdf |
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