Genome-Wide Identification and Analysis of the Plant Cysteine Oxidase (PCO) Gene Family in <i>Brassica napus</i> and Its Role in Abiotic Stress Response
Plant Cysteine Oxidase (PCO) is a plant O<sub>2</sub>-sensing enzyme catalyzing the oxidation of cysteine to Cys-sulfinic acid at the N-termini of target proteins. To better understand the <i>Brassica napus PCO</i> gene family, <i>PCO</i> genes in <i>B. napu...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/14/11242 |
| Summary: | Plant Cysteine Oxidase (PCO) is a plant O<sub>2</sub>-sensing enzyme catalyzing the oxidation of cysteine to Cys-sulfinic acid at the N-termini of target proteins. To better understand the <i>Brassica napus PCO</i> gene family, <i>PCO</i> genes in <i>B. napus</i> and related species were analyzed. In this study, 20, 7 and 8 <i>PCO</i> genes were identified in <i>Brassica napus</i>, <i>Brassica rapa</i> and <i>Brassica oleracea</i>, respectively. According to phylogenetic analysis, the <i>PCOs</i> were divided into five groups: <i>PCO1</i>, <i>PCO2</i>, <i>PCO3</i>, <i>PCO4</i> and <i>PCO5</i>. Gene organization and motif distribution analysis suggested that the <i>PCO</i> gene family was relatively conserved during evolution. According to the public expression data, <i>PCO</i> genes were expressed in different tissues at different developmental stages. Moreover, qRT-PCR data showed that most of the <i>Bna/Bra/BoPCO5</i> members were expressed in leaves, roots, flowers and siliques, suggesting an important role in both vegetative and reproductive development. Expression of <i>BnaPCO</i> was induced by various abiotic stress, especially waterlogging stress, which was consistent with the result of cis-element analysis. In this study, the <i>PCO</i> gene family of <i>Brassicaceae</i> was analyzed for the first time, which contributes to a comprehensive understanding of the origin and evolution of <i>PCO</i> genes in <i>Brassicaceae</i> and the function of <i>BnaPCO</i> in abiotic stress responses. |
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| ISSN: | 1661-6596 1422-0067 |