Prognostic potential of pre-partum blood biochemical and immune variables for postpartum mastitis risk in dairy cows
Abstract Background Mastitis is the most frequent diseases for transition cows. Identification of potential biomarkers for diagnosis of mastitis is important for its prevention. Thus, this study was conducted to investigate blood variables related to lipid metabolism, oxidative stress and inflammati...
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| Format: | Article |
| Language: | English |
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BMC
2020-05-01
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| Series: | BMC Veterinary Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12917-020-02314-6 |
| _version_ | 1831668888166727680 |
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| author | Ruo-wei Guan Di-ming Wang Bei-bei Wang Lu-yi Jiang Jian-xin Liu |
| author_facet | Ruo-wei Guan Di-ming Wang Bei-bei Wang Lu-yi Jiang Jian-xin Liu |
| author_sort | Ruo-wei Guan |
| collection | DOAJ |
| description | Abstract Background Mastitis is the most frequent diseases for transition cows. Identification of potential biomarkers for diagnosis of mastitis is important for its prevention. Thus, this study was conducted to investigate blood variables related to lipid metabolism, oxidative stress and inflammation, and serum variables that are related to health in postpartum cows. Results Seventy-six healthy Holstein dairy cows at week 4 before calving were selected to collect blood samples from weeks − 4 to 4 weekly relative to calving, respectively. Milk yield and composition were recorded weekly. According to the cut-off of somatic cell counts (SCC) for diagnosis of mastitis, 33 cows with SCC ≥ 500,000 cells ml− 1, 20 cows with 200,000 cells ≤ SCC < 500,000 cells ml− 1, and 23 cows with SCC < 200,000 cells ml− 1 were defined as high, middle, and low SCC, respectively. Serum concentrations of β-hydroxybutyrate were higher (P < 0.01) during all weeks, and non-esterified fatty acids were higher in high SCC than in low SCC cows from weeks − 3 to 2 relative to calving. Higher serum concentrations of superoxide dismutase (P < 0.01) and lower malondialdehyde levels (P < 0.01) in low SCC than in high SCC cows indicate that the latter suffered from oxidative stress. The difference analysis of the three groups suggested that none of the above-mentioned variables can be used as potential prognostic candidates. On the other hand, high SCC cows exhibited higher blood neutrophil to lymphocyte ratio (NLR, P < 0.01) and platelet to lymphocyte ratio (PLR, P < 0.01) than low SCC cows, with a higher NLR (P < 0.01) in middle SCC than in low SCC cows. The high SCC cows had lower levels of anti-inflammatory factors including IL-10 (P = 0.05), but higher levels of proinflammatory factors such as IL-6 (P < 0.01), TNF-α (P < 0.05), and PSGL-1 (P < 0.01) than low SCC cows. Conclusions The significantly different NLR and PLR pre-partum between the middle and low SCC cows suggest their prognostic potential for postpartum mastitis risk. |
| first_indexed | 2024-12-19T22:49:23Z |
| format | Article |
| id | doaj.art-669eaafb933b4561a1d16a9944f9ae64 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-19T22:49:23Z |
| publishDate | 2020-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-669eaafb933b4561a1d16a9944f9ae642022-12-21T20:02:52ZengBMCBMC Veterinary Research1746-61482020-05-0116111110.1186/s12917-020-02314-6Prognostic potential of pre-partum blood biochemical and immune variables for postpartum mastitis risk in dairy cowsRuo-wei Guan0Di-ming Wang1Bei-bei Wang2Lu-yi Jiang3Jian-xin Liu4Institute of Dairy Science, College of Animal Sciences, Zhejiang UniversityInstitute of Dairy Science, College of Animal Sciences, Zhejiang UniversityInstitute of Dairy Science, College of Animal Sciences, Zhejiang UniversityInstitute of Dairy Science, College of Animal Sciences, Zhejiang UniversityInstitute of Dairy Science, College of Animal Sciences, Zhejiang UniversityAbstract Background Mastitis is the most frequent diseases for transition cows. Identification of potential biomarkers for diagnosis of mastitis is important for its prevention. Thus, this study was conducted to investigate blood variables related to lipid metabolism, oxidative stress and inflammation, and serum variables that are related to health in postpartum cows. Results Seventy-six healthy Holstein dairy cows at week 4 before calving were selected to collect blood samples from weeks − 4 to 4 weekly relative to calving, respectively. Milk yield and composition were recorded weekly. According to the cut-off of somatic cell counts (SCC) for diagnosis of mastitis, 33 cows with SCC ≥ 500,000 cells ml− 1, 20 cows with 200,000 cells ≤ SCC < 500,000 cells ml− 1, and 23 cows with SCC < 200,000 cells ml− 1 were defined as high, middle, and low SCC, respectively. Serum concentrations of β-hydroxybutyrate were higher (P < 0.01) during all weeks, and non-esterified fatty acids were higher in high SCC than in low SCC cows from weeks − 3 to 2 relative to calving. Higher serum concentrations of superoxide dismutase (P < 0.01) and lower malondialdehyde levels (P < 0.01) in low SCC than in high SCC cows indicate that the latter suffered from oxidative stress. The difference analysis of the three groups suggested that none of the above-mentioned variables can be used as potential prognostic candidates. On the other hand, high SCC cows exhibited higher blood neutrophil to lymphocyte ratio (NLR, P < 0.01) and platelet to lymphocyte ratio (PLR, P < 0.01) than low SCC cows, with a higher NLR (P < 0.01) in middle SCC than in low SCC cows. The high SCC cows had lower levels of anti-inflammatory factors including IL-10 (P = 0.05), but higher levels of proinflammatory factors such as IL-6 (P < 0.01), TNF-α (P < 0.05), and PSGL-1 (P < 0.01) than low SCC cows. Conclusions The significantly different NLR and PLR pre-partum between the middle and low SCC cows suggest their prognostic potential for postpartum mastitis risk.http://link.springer.com/article/10.1186/s12917-020-02314-6Blood biochemical variablesNeutrophil to lymphocyte ratioPlatelet to lymphocyte ratioMastitisTransition cows |
| spellingShingle | Ruo-wei Guan Di-ming Wang Bei-bei Wang Lu-yi Jiang Jian-xin Liu Prognostic potential of pre-partum blood biochemical and immune variables for postpartum mastitis risk in dairy cows BMC Veterinary Research Blood biochemical variables Neutrophil to lymphocyte ratio Platelet to lymphocyte ratio Mastitis Transition cows |
| title | Prognostic potential of pre-partum blood biochemical and immune variables for postpartum mastitis risk in dairy cows |
| title_full | Prognostic potential of pre-partum blood biochemical and immune variables for postpartum mastitis risk in dairy cows |
| title_fullStr | Prognostic potential of pre-partum blood biochemical and immune variables for postpartum mastitis risk in dairy cows |
| title_full_unstemmed | Prognostic potential of pre-partum blood biochemical and immune variables for postpartum mastitis risk in dairy cows |
| title_short | Prognostic potential of pre-partum blood biochemical and immune variables for postpartum mastitis risk in dairy cows |
| title_sort | prognostic potential of pre partum blood biochemical and immune variables for postpartum mastitis risk in dairy cows |
| topic | Blood biochemical variables Neutrophil to lymphocyte ratio Platelet to lymphocyte ratio Mastitis Transition cows |
| url | http://link.springer.com/article/10.1186/s12917-020-02314-6 |
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