Lippia citriodora (verbascoside) extract supplementation: Effect on rabbit semen quality in vivo and in vitro
Verbascoside, the main component of Lippia citriodora extract, is one of the most powerful free radical scavengers exhibiting a wide biological activity. In in vivo study 20 adult New Zealand white rabbit bucks were divided into two homogeneous groups, one control (CON) and one verbascoside-suppleme...
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Czech Academy of Agricultural Sciences
2019-01-01
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| Series: | Czech Journal of Animal Science |
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| Online Access: | https://cjas.agriculturejournals.cz/artkey/cjs-201901-0001_lippia-citriodora-verbascoside-extract-supplementation-effect-on-rabbit-semen-quality-in-vivo-and-in-vitro.php |
| _version_ | 1797899125160673280 |
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| author | Francesco Vizzarri Marisa Palazzo Donato Casamassima Lubomir Ondruska Martin Massanyi Filip Tirpak Grzegorz Formicki Agnieszka Gren Peter Massanyi |
| author_facet | Francesco Vizzarri Marisa Palazzo Donato Casamassima Lubomir Ondruska Martin Massanyi Filip Tirpak Grzegorz Formicki Agnieszka Gren Peter Massanyi |
| author_sort | Francesco Vizzarri |
| collection | DOAJ |
| description | Verbascoside, the main component of Lippia citriodora extract, is one of the most powerful free radical scavengers exhibiting a wide biological activity. In in vivo study 20 adult New Zealand white rabbit bucks were divided into two homogeneous groups, one control (CON) and one verbascoside-supplemented (0.1%) in feed mixture (EXP) and later in vitro effects of verbascoside on the motility aspects of rabbit spermatozoa were analysed. The spermatozoa concentration, ejaculate volume, spermatozoa motility, progressive motility, distance parameters, velocity parameters and type of spermatozoa movement were negatively affected by Lippia citriodora leaves extract after the first 4 weeks of dietary treatment, till the end of experiment (8 weeks). Four weeks after the suspension of feed additive supplementation, all spermatozoa traits values returned to the normality, and in line with CON group. For in vitro findings, ejaculates from 10 male New Zealand white bucks were collected using an artificial vagina. Then it was diluted in physiological saline solution containing different concentrations of verbascoside at the concentration of 0, 0.0024, 0.0219, 0.157, 120.0 mg/ml (Ctrl, VB1, VB2, VB3, VB4 groups, respectively), using a dilution ratio of 1 : 4. The obtained data proved that verbascoside at the concentration of 0.0024 and 0.0219 mg/ml had no adverse effect on spermatozoa. Additionally, we found that verbascoside at higher concentrations (0.157 and 120.0 mg/ml) significantly altered all the motility parameters analysed in the experiment. In conclusion a possible negative effect of verbascoside supplementation into feed mixture (0.1%) on semen quality parameters in rabbit bucks as well as in vitro can be stated, obviously considering that target organs of antioxidant activities of phenylpropanoid glycosides are various. In addition it has to be emphasized that the extract showed a reversible action, since the semen traits of treated animals returned to the normality after the dietary administration period. |
| first_indexed | 2024-04-10T08:25:04Z |
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| institution | Directory Open Access Journal |
| issn | 1212-1819 1805-9309 |
| language | English |
| last_indexed | 2024-04-10T08:25:04Z |
| publishDate | 2019-01-01 |
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| spelling | doaj.art-669f06fc432c4cd3b9bb44e032cdd3ba2023-02-23T03:33:40ZengCzech Academy of Agricultural SciencesCzech Journal of Animal Science1212-18191805-93092019-01-0164111010.17221/35/2018-CJAScjs-201901-0001Lippia citriodora (verbascoside) extract supplementation: Effect on rabbit semen quality in vivo and in vitroFrancesco Vizzarri0Marisa Palazzo1Donato Casamassima2Lubomir Ondruska3Martin Massanyi4Filip Tirpak5Grzegorz Formicki6Agnieszka Gren7Peter Massanyi8Department of Agricultural, Environmental and Food Sciences, University of Molise, Campobasso, ItalyDepartment of Agricultural, Environmental and Food Sciences, University of Molise, Campobasso, ItalyDepartment of Agricultural, Environmental and Food Sciences, University of Molise, Campobasso, ItalyNational Agricultural and Food Centre, Nitra, Slovak RepublicDepartment of Animal Physiology, Slovak University of Agriculture, Nitra, Slovak RepublicDepartment of Animal Physiology, Slovak University of Agriculture, Nitra, Slovak RepublicInstitute of Biology, Pedagogical University, Krakow, PolandInstitute of Biology, Pedagogical University, Krakow, PolandDepartment of Animal Physiology, Slovak University of Agriculture, Nitra, Slovak RepublicVerbascoside, the main component of Lippia citriodora extract, is one of the most powerful free radical scavengers exhibiting a wide biological activity. In in vivo study 20 adult New Zealand white rabbit bucks were divided into two homogeneous groups, one control (CON) and one verbascoside-supplemented (0.1%) in feed mixture (EXP) and later in vitro effects of verbascoside on the motility aspects of rabbit spermatozoa were analysed. The spermatozoa concentration, ejaculate volume, spermatozoa motility, progressive motility, distance parameters, velocity parameters and type of spermatozoa movement were negatively affected by Lippia citriodora leaves extract after the first 4 weeks of dietary treatment, till the end of experiment (8 weeks). Four weeks after the suspension of feed additive supplementation, all spermatozoa traits values returned to the normality, and in line with CON group. For in vitro findings, ejaculates from 10 male New Zealand white bucks were collected using an artificial vagina. Then it was diluted in physiological saline solution containing different concentrations of verbascoside at the concentration of 0, 0.0024, 0.0219, 0.157, 120.0 mg/ml (Ctrl, VB1, VB2, VB3, VB4 groups, respectively), using a dilution ratio of 1 : 4. The obtained data proved that verbascoside at the concentration of 0.0024 and 0.0219 mg/ml had no adverse effect on spermatozoa. Additionally, we found that verbascoside at higher concentrations (0.157 and 120.0 mg/ml) significantly altered all the motility parameters analysed in the experiment. In conclusion a possible negative effect of verbascoside supplementation into feed mixture (0.1%) on semen quality parameters in rabbit bucks as well as in vitro can be stated, obviously considering that target organs of antioxidant activities of phenylpropanoid glycosides are various. In addition it has to be emphasized that the extract showed a reversible action, since the semen traits of treated animals returned to the normality after the dietary administration period.https://cjas.agriculturejournals.cz/artkey/cjs-201901-0001_lippia-citriodora-verbascoside-extract-supplementation-effect-on-rabbit-semen-quality-in-vivo-and-in-vitro.phpfeed additiveverbenaceaerabbitspermatozoamotility |
| spellingShingle | Francesco Vizzarri Marisa Palazzo Donato Casamassima Lubomir Ondruska Martin Massanyi Filip Tirpak Grzegorz Formicki Agnieszka Gren Peter Massanyi Lippia citriodora (verbascoside) extract supplementation: Effect on rabbit semen quality in vivo and in vitro Czech Journal of Animal Science feed additive verbenaceae rabbit spermatozoa motility |
| title | Lippia citriodora (verbascoside) extract supplementation: Effect on rabbit semen quality in vivo and in vitro |
| title_full | Lippia citriodora (verbascoside) extract supplementation: Effect on rabbit semen quality in vivo and in vitro |
| title_fullStr | Lippia citriodora (verbascoside) extract supplementation: Effect on rabbit semen quality in vivo and in vitro |
| title_full_unstemmed | Lippia citriodora (verbascoside) extract supplementation: Effect on rabbit semen quality in vivo and in vitro |
| title_short | Lippia citriodora (verbascoside) extract supplementation: Effect on rabbit semen quality in vivo and in vitro |
| title_sort | lippia citriodora verbascoside extract supplementation effect on rabbit semen quality in vivo and in vitro |
| topic | feed additive verbenaceae rabbit spermatozoa motility |
| url | https://cjas.agriculturejournals.cz/artkey/cjs-201901-0001_lippia-citriodora-verbascoside-extract-supplementation-effect-on-rabbit-semen-quality-in-vivo-and-in-vitro.php |
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