Plasticity of Arabidopsis rosette transcriptomes and photosynthetic responses in dynamic light conditions
Abstract With the high variability of natural growth environments, plants exhibit flexibility and resilience in regard to the strategies they employ to maintain overall fitness, including maximizing light use for photosynthesis, while simultaneously limiting light‐associated damage. We measured dist...
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| Format: | Article |
| Language: | English |
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Wiley
2023-01-01
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| Series: | Plant Direct |
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| Online Access: | https://doi.org/10.1002/pld3.475 |
| _version_ | 1828049076173668352 |
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| author | Hussien F. Alameldin Beronda L. Montgomery |
| author_facet | Hussien F. Alameldin Beronda L. Montgomery |
| author_sort | Hussien F. Alameldin |
| collection | DOAJ |
| description | Abstract With the high variability of natural growth environments, plants exhibit flexibility and resilience in regard to the strategies they employ to maintain overall fitness, including maximizing light use for photosynthesis, while simultaneously limiting light‐associated damage. We measured distinct parameters of photosynthetic performance of Arabidopsis thaliana plants under dynamic light regimes. Plants were grown to maturity then subjected to the following 5‐day (16 h light, 8 h dark) regime: Day 1 at constant light (CL) intensity during light period, representative of a common lab growth condition; Day 2 under sinusoidal variation in light intensity (SL) during the light period that is representative of changes occurring during a clear sunny day; Day 3 under fluctuating light (FL) intensity during the light period that simulates sudden changes that might occur with the movements of clouds in and out of the view of the sun; Day 4, repeat of CL; and Day 5, repeat of FL. We also examined the global transcriptome profile in these growth conditions based on obtaining RNA‐sequencing (RNA‐seq) data for whole plant rosettes. Our transcriptomic analyses indicated downregulation of photosystem I (PSI) and II (PSII) associated genes, which were correlated with elevated levels of photoinhibition as indicated by measurements of nonphotochemical quenching (NPQ), energy‐dependent quenching (qE), and inhibitory quenching (qI) under both SL and FL conditions. Furthermore, our transcriptomic results indicated downregulation of tetrapyrrole biosynthesis associated genes, coupled with reduced levels of chlorophyll under both SL and FL compared with CL, as well as downregulation of photorespiration‐associated genes under SL. We also noticed an enrichment of the stress response gene ontology (GO) terms for genes differentially regulated under FL when compared with SL. Collectively, our phenotypic and transcriptome analyses serve as useful resources for probing the underlying molecular mechanisms associated with plant acclimation to rapid light intensity changes in the natural environment. |
| first_indexed | 2024-04-10T19:06:41Z |
| format | Article |
| id | doaj.art-669fe2f62c0c4d638124195f785ae350 |
| institution | Directory Open Access Journal |
| issn | 2475-4455 |
| language | English |
| last_indexed | 2024-04-10T19:06:41Z |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Plant Direct |
| spelling | doaj.art-669fe2f62c0c4d638124195f785ae3502023-01-31T00:12:54ZengWileyPlant Direct2475-44552023-01-0171n/an/a10.1002/pld3.475Plasticity of Arabidopsis rosette transcriptomes and photosynthetic responses in dynamic light conditionsHussien F. Alameldin0Beronda L. Montgomery1DOE‐Plant Research Laboratory Michigan State University East Lansing Michigan USADOE‐Plant Research Laboratory Michigan State University East Lansing Michigan USAAbstract With the high variability of natural growth environments, plants exhibit flexibility and resilience in regard to the strategies they employ to maintain overall fitness, including maximizing light use for photosynthesis, while simultaneously limiting light‐associated damage. We measured distinct parameters of photosynthetic performance of Arabidopsis thaliana plants under dynamic light regimes. Plants were grown to maturity then subjected to the following 5‐day (16 h light, 8 h dark) regime: Day 1 at constant light (CL) intensity during light period, representative of a common lab growth condition; Day 2 under sinusoidal variation in light intensity (SL) during the light period that is representative of changes occurring during a clear sunny day; Day 3 under fluctuating light (FL) intensity during the light period that simulates sudden changes that might occur with the movements of clouds in and out of the view of the sun; Day 4, repeat of CL; and Day 5, repeat of FL. We also examined the global transcriptome profile in these growth conditions based on obtaining RNA‐sequencing (RNA‐seq) data for whole plant rosettes. Our transcriptomic analyses indicated downregulation of photosystem I (PSI) and II (PSII) associated genes, which were correlated with elevated levels of photoinhibition as indicated by measurements of nonphotochemical quenching (NPQ), energy‐dependent quenching (qE), and inhibitory quenching (qI) under both SL and FL conditions. Furthermore, our transcriptomic results indicated downregulation of tetrapyrrole biosynthesis associated genes, coupled with reduced levels of chlorophyll under both SL and FL compared with CL, as well as downregulation of photorespiration‐associated genes under SL. We also noticed an enrichment of the stress response gene ontology (GO) terms for genes differentially regulated under FL when compared with SL. Collectively, our phenotypic and transcriptome analyses serve as useful resources for probing the underlying molecular mechanisms associated with plant acclimation to rapid light intensity changes in the natural environment.https://doi.org/10.1002/pld3.475fluctuating lightlight acclimationphotoinhibitionphotosynthesissinusoidal lighttetrapyrrole biosynthesis |
| spellingShingle | Hussien F. Alameldin Beronda L. Montgomery Plasticity of Arabidopsis rosette transcriptomes and photosynthetic responses in dynamic light conditions Plant Direct fluctuating light light acclimation photoinhibition photosynthesis sinusoidal light tetrapyrrole biosynthesis |
| title | Plasticity of Arabidopsis rosette transcriptomes and photosynthetic responses in dynamic light conditions |
| title_full | Plasticity of Arabidopsis rosette transcriptomes and photosynthetic responses in dynamic light conditions |
| title_fullStr | Plasticity of Arabidopsis rosette transcriptomes and photosynthetic responses in dynamic light conditions |
| title_full_unstemmed | Plasticity of Arabidopsis rosette transcriptomes and photosynthetic responses in dynamic light conditions |
| title_short | Plasticity of Arabidopsis rosette transcriptomes and photosynthetic responses in dynamic light conditions |
| title_sort | plasticity of arabidopsis rosette transcriptomes and photosynthetic responses in dynamic light conditions |
| topic | fluctuating light light acclimation photoinhibition photosynthesis sinusoidal light tetrapyrrole biosynthesis |
| url | https://doi.org/10.1002/pld3.475 |
| work_keys_str_mv | AT hussienfalameldin plasticityofarabidopsisrosettetranscriptomesandphotosyntheticresponsesindynamiclightconditions AT berondalmontgomery plasticityofarabidopsisrosettetranscriptomesandphotosyntheticresponsesindynamiclightconditions |