Overexpression of circulating CD38+ NK cells in colorectal cancer was associated with lymph node metastasis and poor prognosis
IntroductionLymph node metastasis (LNM) is a critical prognostic factor for colorectal cancer (CRC). Due to the potential influence of immune system on CRC progression, investigation into lymphocyte subsets as clinical markers has gained attention. The objective of this study was to assess the capab...
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| Format: | Article |
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Frontiers Media S.A.
2024-02-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2024.1309785/full |
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| author | Xueling Wang Haoran Li Huixian Chen Kehua Fang Xiaotian Chang |
| author_facet | Xueling Wang Haoran Li Huixian Chen Kehua Fang Xiaotian Chang |
| author_sort | Xueling Wang |
| collection | DOAJ |
| description | IntroductionLymph node metastasis (LNM) is a critical prognostic factor for colorectal cancer (CRC). Due to the potential influence of immune system on CRC progression, investigation into lymphocyte subsets as clinical markers has gained attention. The objective of this study was to assess the capability of lymphocyte subsets in evaluating the lymph node status and prognosis of CRC.MethodsLymphocyte subsets, including T cells (CD3+), natural killer cells (NK, CD3- CD56+), natural killer-like T cells (NK-like T, CD3+ CD56+), CD38+ NK cells (CD3- CD56+ CD38+) and CD38+ NK-like T cells (CD3+ CD56+ CD38+), were detected by flow cytometry. Univariate and multivariate analyses were used to assess the risk factors of LNM. The prognostic role of parameters was evaluated by survival analysis.ResultsThe proportion of CD38+ NK cells within the NK cell population was significantly higher in LNM-positive patients (p <0.0001). However, no significant differences were observed in the proportions of other lymphocyte subsets. Poorer histologic grade (odds ratio [OR] =4.76, p =0.03), lymphovascular invasion (LVI) (OR =22.38, p <0.01), and CD38+ NK cells (high) (OR =4.54, p <0.01) were identified as independent risk factors for LNM. Furthermore, high proportion of CD38+ NK cells was associated with poor prognosis of CRC patients (HR=2.37, p =0.03).ConclusionsIt was demonstrated that the proportion of CD38+ NK cells was a marker overexpressed in LNM-positive patients compared with LNM-negative patients. Moreover, an elevated proportion of CD38+ NK cells is a risk factor for LNM and poor prognosis in CRC. |
| first_indexed | 2024-03-07T22:49:24Z |
| format | Article |
| id | doaj.art-66a04319d9c14817924eafd7751efe05 |
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| issn | 2234-943X |
| language | English |
| last_indexed | 2024-03-07T22:49:24Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-66a04319d9c14817924eafd7751efe052024-02-23T11:25:21ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2024-02-011410.3389/fonc.2024.13097851309785Overexpression of circulating CD38+ NK cells in colorectal cancer was associated with lymph node metastasis and poor prognosisXueling Wang0Haoran Li1Huixian Chen2Kehua Fang3Xiaotian Chang4Center for Clinical Research, The Affiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Hepatobiliary and Pancreatic Surgery, The Affiliated Hospital of Qingdao University, Qingdao, ChinaCenter for Clinical Research, The Affiliated Hospital of Qingdao University, Qingdao, ChinaClinical Laboratory, The Affiliated Hospital of Qingdao University, Qingdao, ChinaCenter for Clinical Research, The Affiliated Hospital of Qingdao University, Qingdao, ChinaIntroductionLymph node metastasis (LNM) is a critical prognostic factor for colorectal cancer (CRC). Due to the potential influence of immune system on CRC progression, investigation into lymphocyte subsets as clinical markers has gained attention. The objective of this study was to assess the capability of lymphocyte subsets in evaluating the lymph node status and prognosis of CRC.MethodsLymphocyte subsets, including T cells (CD3+), natural killer cells (NK, CD3- CD56+), natural killer-like T cells (NK-like T, CD3+ CD56+), CD38+ NK cells (CD3- CD56+ CD38+) and CD38+ NK-like T cells (CD3+ CD56+ CD38+), were detected by flow cytometry. Univariate and multivariate analyses were used to assess the risk factors of LNM. The prognostic role of parameters was evaluated by survival analysis.ResultsThe proportion of CD38+ NK cells within the NK cell population was significantly higher in LNM-positive patients (p <0.0001). However, no significant differences were observed in the proportions of other lymphocyte subsets. Poorer histologic grade (odds ratio [OR] =4.76, p =0.03), lymphovascular invasion (LVI) (OR =22.38, p <0.01), and CD38+ NK cells (high) (OR =4.54, p <0.01) were identified as independent risk factors for LNM. Furthermore, high proportion of CD38+ NK cells was associated with poor prognosis of CRC patients (HR=2.37, p =0.03).ConclusionsIt was demonstrated that the proportion of CD38+ NK cells was a marker overexpressed in LNM-positive patients compared with LNM-negative patients. Moreover, an elevated proportion of CD38+ NK cells is a risk factor for LNM and poor prognosis in CRC.https://www.frontiersin.org/articles/10.3389/fonc.2024.1309785/fullCD38+ NK cellscolorectal cancerlymph node metastasisprognosismarker |
| spellingShingle | Xueling Wang Haoran Li Huixian Chen Kehua Fang Xiaotian Chang Overexpression of circulating CD38+ NK cells in colorectal cancer was associated with lymph node metastasis and poor prognosis Frontiers in Oncology CD38+ NK cells colorectal cancer lymph node metastasis prognosis marker |
| title | Overexpression of circulating CD38+ NK cells in colorectal cancer was associated with lymph node metastasis and poor prognosis |
| title_full | Overexpression of circulating CD38+ NK cells in colorectal cancer was associated with lymph node metastasis and poor prognosis |
| title_fullStr | Overexpression of circulating CD38+ NK cells in colorectal cancer was associated with lymph node metastasis and poor prognosis |
| title_full_unstemmed | Overexpression of circulating CD38+ NK cells in colorectal cancer was associated with lymph node metastasis and poor prognosis |
| title_short | Overexpression of circulating CD38+ NK cells in colorectal cancer was associated with lymph node metastasis and poor prognosis |
| title_sort | overexpression of circulating cd38 nk cells in colorectal cancer was associated with lymph node metastasis and poor prognosis |
| topic | CD38+ NK cells colorectal cancer lymph node metastasis prognosis marker |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2024.1309785/full |
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