Correlation of chromosome damage and promoter methylation status of the DNA repair genes MGMT and hMLH1 in Chinese vinyl chloride monomer (VCM)-exposed workers
Objective: To explore the association of the methylation status of MGMT and hMLH1 with chromosome damage induced by vinyl chloride monomer (VCM). Materials and Methods: Methylation of MGMT and hMLH1 was measured in 101 VCM-exposed workers by methylation-specifi c PCR. Chromosome damage in peripheral...
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| Format: | Article |
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Nofer Institute of Occupational Medicine
2013-02-01
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| Series: | International Journal of Occupational Medicine and Environmental Health |
| Subjects: | |
| Online Access: | http://ijomeh.eu/Correlation-of-chromosome-damage-and-promoter-methylation-status-of-the-dna-repair-genes-mgmt-and-hmlh1-in-chinese-vinyl-chloride-monomer-vcm-exposed-workers,2225,0,2.html |
| _version_ | 1818129108156022784 |
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| author | Fen Wu Jing Liu Yu-Lan Qiu Wei Wang Shou-Min Zhu Pin Sun Wen-Bin Miao Yong-Liang Li Paul W. Brandt-Rauf Zhao-Lin Xia |
| author_facet | Fen Wu Jing Liu Yu-Lan Qiu Wei Wang Shou-Min Zhu Pin Sun Wen-Bin Miao Yong-Liang Li Paul W. Brandt-Rauf Zhao-Lin Xia |
| author_sort | Fen Wu |
| collection | DOAJ |
| description | Objective: To explore the association of the methylation status of MGMT and hMLH1 with chromosome damage induced by vinyl chloride monomer (VCM). Materials and Methods: Methylation of MGMT and hMLH1 was measured in 101 VCM-exposed workers by methylation-specifi c PCR. Chromosome damage in peripheral blood lymphocytes was measured by the cytokinesis-block micronucleus assay. The subjects were divided into chromosome damaged and non-damaged groups based on the normal reference value of micronuclei frequencies determined for two control groups. Results: MGMT promoter methylation was detectable in 5 out of 49 chromosome damaged subjects, but not in the chromosome non-damaged subjects; there was a signifi cant difference in MGMT methylation between the two groups (p < 0.05). Conclusions: We detected aberrant promoter methylation of MGMT in a small number of chromosome damaged VCM-exposed workers, but not in the chromosome non-damaged subjects. This preliminary observation warrants further investigation in a larger study. |
| first_indexed | 2024-12-11T07:43:54Z |
| format | Article |
| id | doaj.art-66a0e81ef0b44538a56e1a692cb3809c |
| institution | Directory Open Access Journal |
| issn | 1232-1087 1896-494X |
| language | English |
| last_indexed | 2024-12-11T07:43:54Z |
| publishDate | 2013-02-01 |
| publisher | Nofer Institute of Occupational Medicine |
| record_format | Article |
| series | International Journal of Occupational Medicine and Environmental Health |
| spelling | doaj.art-66a0e81ef0b44538a56e1a692cb3809c2022-12-22T01:15:30ZengNofer Institute of Occupational MedicineInternational Journal of Occupational Medicine and Environmental Health1232-10871896-494X2013-02-0126117318210.2478/S13382-013-0079-1Correlation of chromosome damage and promoter methylation status of the DNA repair genes MGMT and hMLH1 in Chinese vinyl chloride monomer (VCM)-exposed workersFen WuJing LiuYu-Lan QiuWei WangShou-Min ZhuPin SunWen-Bin MiaoYong-Liang LiPaul W. Brandt-RaufZhao-Lin XiaObjective: To explore the association of the methylation status of MGMT and hMLH1 with chromosome damage induced by vinyl chloride monomer (VCM). Materials and Methods: Methylation of MGMT and hMLH1 was measured in 101 VCM-exposed workers by methylation-specifi c PCR. Chromosome damage in peripheral blood lymphocytes was measured by the cytokinesis-block micronucleus assay. The subjects were divided into chromosome damaged and non-damaged groups based on the normal reference value of micronuclei frequencies determined for two control groups. Results: MGMT promoter methylation was detectable in 5 out of 49 chromosome damaged subjects, but not in the chromosome non-damaged subjects; there was a signifi cant difference in MGMT methylation between the two groups (p < 0.05). Conclusions: We detected aberrant promoter methylation of MGMT in a small number of chromosome damaged VCM-exposed workers, but not in the chromosome non-damaged subjects. This preliminary observation warrants further investigation in a larger study.http://ijomeh.eu/Correlation-of-chromosome-damage-and-promoter-methylation-status-of-the-dna-repair-genes-mgmt-and-hmlh1-in-chinese-vinyl-chloride-monomer-vcm-exposed-workers,2225,0,2.htmlvinyl chloride monomerchromosome damageMGMThMLH1DNA methylation |
| spellingShingle | Fen Wu Jing Liu Yu-Lan Qiu Wei Wang Shou-Min Zhu Pin Sun Wen-Bin Miao Yong-Liang Li Paul W. Brandt-Rauf Zhao-Lin Xia Correlation of chromosome damage and promoter methylation status of the DNA repair genes MGMT and hMLH1 in Chinese vinyl chloride monomer (VCM)-exposed workers International Journal of Occupational Medicine and Environmental Health vinyl chloride monomer chromosome damage MGMT hMLH1 DNA methylation |
| title | Correlation of chromosome damage and promoter methylation status of the DNA repair genes MGMT and hMLH1 in Chinese vinyl chloride monomer (VCM)-exposed workers |
| title_full | Correlation of chromosome damage and promoter methylation status of the DNA repair genes MGMT and hMLH1 in Chinese vinyl chloride monomer (VCM)-exposed workers |
| title_fullStr | Correlation of chromosome damage and promoter methylation status of the DNA repair genes MGMT and hMLH1 in Chinese vinyl chloride monomer (VCM)-exposed workers |
| title_full_unstemmed | Correlation of chromosome damage and promoter methylation status of the DNA repair genes MGMT and hMLH1 in Chinese vinyl chloride monomer (VCM)-exposed workers |
| title_short | Correlation of chromosome damage and promoter methylation status of the DNA repair genes MGMT and hMLH1 in Chinese vinyl chloride monomer (VCM)-exposed workers |
| title_sort | correlation of chromosome damage and promoter methylation status of the dna repair genes mgmt and hmlh1 in chinese vinyl chloride monomer vcm exposed workers |
| topic | vinyl chloride monomer chromosome damage MGMT hMLH1 DNA methylation |
| url | http://ijomeh.eu/Correlation-of-chromosome-damage-and-promoter-methylation-status-of-the-dna-repair-genes-mgmt-and-hmlh1-in-chinese-vinyl-chloride-monomer-vcm-exposed-workers,2225,0,2.html |
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