Microtomographic investigation of a large corpus of cichlids.

A large collection of cichlids (N = 133) from Lake Victoria in Africa, with total lengths ranging from 6 to 18 cm was nondestructively imaged using micro-computed tomography. We present a method to efficiently obtain three-dimensional tomographic datasets of the oral and pharyngeal jaws and the whol...

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Main Authors: David Haberthür, Mikki Law, Kassandra Ford, Marcel Häsler, Ole Seehausen, Ruslan Hlushchuk
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2023-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0291003
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author David Haberthür
Mikki Law
Kassandra Ford
Marcel Häsler
Ole Seehausen
Ruslan Hlushchuk
author_facet David Haberthür
Mikki Law
Kassandra Ford
Marcel Häsler
Ole Seehausen
Ruslan Hlushchuk
author_sort David Haberthür
collection DOAJ
description A large collection of cichlids (N = 133) from Lake Victoria in Africa, with total lengths ranging from 6 to 18 cm was nondestructively imaged using micro-computed tomography. We present a method to efficiently obtain three-dimensional tomographic datasets of the oral and pharyngeal jaws and the whole skull of these fishes to accurately describe their morphology. The tomographic data we acquired (9.8 TB of projection images) yielded 1.5 TB of three-dimensional image stacks used for extracting the relevant features of interest. Herein we present our method and outlooks on analyzing the acquired data; a morphological description of the oral and pharyngeal jaws, a three-dimensional geometric morphometrics analysis of landmarked skull features, and a robust method to automatically extract otoliths from the tomographic data.
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spelling doaj.art-66a1b612de6a478da3ad4da280069c1f2023-10-02T12:18:39ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-01189e029100310.1371/journal.pone.0291003Microtomographic investigation of a large corpus of cichlids.David HaberthürMikki LawKassandra FordMarcel HäslerOle SeehausenRuslan HlushchukA large collection of cichlids (N = 133) from Lake Victoria in Africa, with total lengths ranging from 6 to 18 cm was nondestructively imaged using micro-computed tomography. We present a method to efficiently obtain three-dimensional tomographic datasets of the oral and pharyngeal jaws and the whole skull of these fishes to accurately describe their morphology. The tomographic data we acquired (9.8 TB of projection images) yielded 1.5 TB of three-dimensional image stacks used for extracting the relevant features of interest. Herein we present our method and outlooks on analyzing the acquired data; a morphological description of the oral and pharyngeal jaws, a three-dimensional geometric morphometrics analysis of landmarked skull features, and a robust method to automatically extract otoliths from the tomographic data.https://doi.org/10.1371/journal.pone.0291003
spellingShingle David Haberthür
Mikki Law
Kassandra Ford
Marcel Häsler
Ole Seehausen
Ruslan Hlushchuk
Microtomographic investigation of a large corpus of cichlids.
PLoS ONE
title Microtomographic investigation of a large corpus of cichlids.
title_full Microtomographic investigation of a large corpus of cichlids.
title_fullStr Microtomographic investigation of a large corpus of cichlids.
title_full_unstemmed Microtomographic investigation of a large corpus of cichlids.
title_short Microtomographic investigation of a large corpus of cichlids.
title_sort microtomographic investigation of a large corpus of cichlids
url https://doi.org/10.1371/journal.pone.0291003
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