Detection of miRNA using a surface plasmon resonance biosensor and antibody amplification
MiRNAs are non-coding RNA molecules that control biological functions by reducing the translation of target proteins when binding to the mRNA. Alterations of the miRNA expression profile affect the cell metabolism, which can lead to distinctive disease patterns thus suggesting miRNA as an interestin...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2016-09-01
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| Series: | Current Directions in Biomedical Engineering |
| Subjects: | |
| Online Access: | http://www.degruyter.com/view/j/cdbme.2016.2.issue-1/cdbme-2016-0032/cdbme-2016-0032.xml?format=INT |
| _version_ | 1811239345926963200 |
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| author | Schmieder Stefan Weißpflog Janek Danz Norbert Klotzbach Udo Sonntag Frank |
| author_facet | Schmieder Stefan Weißpflog Janek Danz Norbert Klotzbach Udo Sonntag Frank |
| author_sort | Schmieder Stefan |
| collection | DOAJ |
| description | MiRNAs are non-coding RNA molecules that control biological functions by reducing the translation of target proteins when binding to the mRNA. Alterations of the miRNA expression profile affect the cell metabolism, which can lead to distinctive disease patterns thus suggesting miRNA as an interesting biomarker. Here we present a SPR biosensor that utilizes disposable, injection-molded sensor chip/microfluidic hybrids combined with a lateral imaging optical system for parallel analysis of three one-dimensional spot arrays to detect miRNA-93. Using a RNA-DNA-hybrid antibody for signal enhancement we could reach a limit of detection of 10 pmol/l. |
| first_indexed | 2024-04-12T12:59:23Z |
| format | Article |
| id | doaj.art-66a512e2777346a2a75b8b4390e36ef8 |
| institution | Directory Open Access Journal |
| issn | 2364-5504 |
| language | English |
| last_indexed | 2024-04-12T12:59:23Z |
| publishDate | 2016-09-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Current Directions in Biomedical Engineering |
| spelling | doaj.art-66a512e2777346a2a75b8b4390e36ef82022-12-22T03:32:14ZengDe GruyterCurrent Directions in Biomedical Engineering2364-55042016-09-012113513810.1515/cdbme-2016-0032cdbme-2016-0032Detection of miRNA using a surface plasmon resonance biosensor and antibody amplificationSchmieder Stefan0Weißpflog Janek1Danz Norbert2Klotzbach Udo3Sonntag Frank4Fraunhofer Institute for Material and Beam Technology IWS, Winterbergstraße 28, 01277 Dresden, GermanyFraunhofer Institute for Material and Beam Technology IWS, Winterbergstraße 28, 01277 Dresden, GermanyFraunhofer Institute for Applied Optics and Precision Engineering IOF, Albert-Einstein-Str. 7, 07745 Jena, GermanyFraunhofer Institute for Material and Beam Technology IWS, Winterbergstraße 28, 01277 Dresden, GermanyFraunhofer Institute for Material and Beam Technology IWS, Winterbergstraße 28, 01277 Dresden, GermanyMiRNAs are non-coding RNA molecules that control biological functions by reducing the translation of target proteins when binding to the mRNA. Alterations of the miRNA expression profile affect the cell metabolism, which can lead to distinctive disease patterns thus suggesting miRNA as an interesting biomarker. Here we present a SPR biosensor that utilizes disposable, injection-molded sensor chip/microfluidic hybrids combined with a lateral imaging optical system for parallel analysis of three one-dimensional spot arrays to detect miRNA-93. Using a RNA-DNA-hybrid antibody for signal enhancement we could reach a limit of detection of 10 pmol/l.http://www.degruyter.com/view/j/cdbme.2016.2.issue-1/cdbme-2016-0032/cdbme-2016-0032.xml?format=INTbiochipbiosensormiRNAmolecular diagnosticSPRsurface plasmon resonance |
| spellingShingle | Schmieder Stefan Weißpflog Janek Danz Norbert Klotzbach Udo Sonntag Frank Detection of miRNA using a surface plasmon resonance biosensor and antibody amplification Current Directions in Biomedical Engineering biochip biosensor miRNA molecular diagnostic SPR surface plasmon resonance |
| title | Detection of miRNA using a surface plasmon resonance biosensor and antibody amplification |
| title_full | Detection of miRNA using a surface plasmon resonance biosensor and antibody amplification |
| title_fullStr | Detection of miRNA using a surface plasmon resonance biosensor and antibody amplification |
| title_full_unstemmed | Detection of miRNA using a surface plasmon resonance biosensor and antibody amplification |
| title_short | Detection of miRNA using a surface plasmon resonance biosensor and antibody amplification |
| title_sort | detection of mirna using a surface plasmon resonance biosensor and antibody amplification |
| topic | biochip biosensor miRNA molecular diagnostic SPR surface plasmon resonance |
| url | http://www.degruyter.com/view/j/cdbme.2016.2.issue-1/cdbme-2016-0032/cdbme-2016-0032.xml?format=INT |
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