Occurrence of Cryptosporidium spp. oocytes in artificial beaches of Itaipu Lake, Paraná, Brazil
Cryptosporidiosis is an emerging zoonosis that causes many outbreaks worldwide. Its main route of transmission is the consumption of contaminated water or food. In Brazil, data on to the occurrence of cryptosporidiosis in animals and in humans are scarce; thus, it is important to determine the occur...
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Londrina
2017-06-01
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| Series: | Semina: Ciências Agrárias |
| Subjects: | |
| Online Access: | http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/24923 |
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| author | Alessandra Snak Pedro Rodrigo Hillesheim Soares Erton Gomes da Silva Mallú Jagnow Sereno Felipe Gustavo Garcia Marcia Benedita de Oliveira Silva Silvia Cristina Osaki |
| author_facet | Alessandra Snak Pedro Rodrigo Hillesheim Soares Erton Gomes da Silva Mallú Jagnow Sereno Felipe Gustavo Garcia Marcia Benedita de Oliveira Silva Silvia Cristina Osaki |
| author_sort | Alessandra Snak |
| collection | DOAJ |
| description | Cryptosporidiosis is an emerging zoonosis that causes many outbreaks worldwide. Its main route of transmission is the consumption of contaminated water or food. In Brazil, data on to the occurrence of cryptosporidiosis in animals and in humans are scarce; thus, it is important to determine the occurrence of Cryptosporidium sp. and to identify possible sources of water and food contamination in order to prevent the disease and, consequently, preserve of human health. From July/2011 to March/2012, five liters of water were collected in 10 different locations from eight artificial beaches of Itaipu Lake, Paraná, in a total of 40 samples. In two other small beaches of the lake, water was also collected from two different sites of each beach, given their size and because they were made up of water from different sources. Samples were analyzed by the modified Ziehl-Neelsen technique and nested-PCR (nPCR). All samples were negative in the modified Ziehl-Neelsen; only one (2.25%) sample was positive in nPCR. These results indicate that PCR is a more sensitive technique for the identification of Cryptosporidium in water samples. In this study, it was not possible to identify the species involved and, thus, the source of infection. Water contamination by Cryptosporidium oocytes can be caused by livestock kept near waterways, and by the people who use the place for recreational purposes. These results demonstrate risk for people who use the lake. Care should be determined to prevent the transmission of this important zoonosis. |
| first_indexed | 2024-12-14T13:10:53Z |
| format | Article |
| id | doaj.art-66a926e0844443b5ac973eaa04f43077 |
| institution | Directory Open Access Journal |
| issn | 1676-546X 1679-0359 |
| language | English |
| last_indexed | 2024-12-14T13:10:53Z |
| publishDate | 2017-06-01 |
| publisher | Universidade Estadual de Londrina |
| record_format | Article |
| series | Semina: Ciências Agrárias |
| spelling | doaj.art-66a926e0844443b5ac973eaa04f430772022-12-21T23:00:12ZengUniversidade Estadual de LondrinaSemina: Ciências Agrárias1676-546X1679-03592017-06-013831657166410.5433/1679-0359.2017v38n3p165715351Occurrence of Cryptosporidium spp. oocytes in artificial beaches of Itaipu Lake, Paraná, BrazilAlessandra Snak0Pedro Rodrigo Hillesheim Soares1Erton Gomes da Silva2Mallú Jagnow Sereno3Felipe Gustavo Garcia4Marcia Benedita de Oliveira Silva5Silvia Cristina Osaki6Universidade do Estado de Santa CatarinaUniversidade Federal do ParanáUniversidade Federal do ParanáUniversidade Federal do ParanáUniversidade Federal do ParanáUniversidade Federal do Triangulo MineiroUniversidade Federal do ParanáCryptosporidiosis is an emerging zoonosis that causes many outbreaks worldwide. Its main route of transmission is the consumption of contaminated water or food. In Brazil, data on to the occurrence of cryptosporidiosis in animals and in humans are scarce; thus, it is important to determine the occurrence of Cryptosporidium sp. and to identify possible sources of water and food contamination in order to prevent the disease and, consequently, preserve of human health. From July/2011 to March/2012, five liters of water were collected in 10 different locations from eight artificial beaches of Itaipu Lake, Paraná, in a total of 40 samples. In two other small beaches of the lake, water was also collected from two different sites of each beach, given their size and because they were made up of water from different sources. Samples were analyzed by the modified Ziehl-Neelsen technique and nested-PCR (nPCR). All samples were negative in the modified Ziehl-Neelsen; only one (2.25%) sample was positive in nPCR. These results indicate that PCR is a more sensitive technique for the identification of Cryptosporidium in water samples. In this study, it was not possible to identify the species involved and, thus, the source of infection. Water contamination by Cryptosporidium oocytes can be caused by livestock kept near waterways, and by the people who use the place for recreational purposes. These results demonstrate risk for people who use the lake. Care should be determined to prevent the transmission of this important zoonosis.http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/24923CryptosporidiumPCRWaterZiehl-Neelsen. |
| spellingShingle | Alessandra Snak Pedro Rodrigo Hillesheim Soares Erton Gomes da Silva Mallú Jagnow Sereno Felipe Gustavo Garcia Marcia Benedita de Oliveira Silva Silvia Cristina Osaki Occurrence of Cryptosporidium spp. oocytes in artificial beaches of Itaipu Lake, Paraná, Brazil Semina: Ciências Agrárias Cryptosporidium PCR Water Ziehl-Neelsen. |
| title | Occurrence of Cryptosporidium spp. oocytes in artificial beaches of Itaipu Lake, Paraná, Brazil |
| title_full | Occurrence of Cryptosporidium spp. oocytes in artificial beaches of Itaipu Lake, Paraná, Brazil |
| title_fullStr | Occurrence of Cryptosporidium spp. oocytes in artificial beaches of Itaipu Lake, Paraná, Brazil |
| title_full_unstemmed | Occurrence of Cryptosporidium spp. oocytes in artificial beaches of Itaipu Lake, Paraná, Brazil |
| title_short | Occurrence of Cryptosporidium spp. oocytes in artificial beaches of Itaipu Lake, Paraná, Brazil |
| title_sort | occurrence of cryptosporidium spp oocytes in artificial beaches of itaipu lake parana brazil |
| topic | Cryptosporidium PCR Water Ziehl-Neelsen. |
| url | http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/24923 |
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